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The map label for this gene is rsmE [H]

Identifier: 229220796

GI number: 229220796

Start: 707527

End: 708294

Strand: Reverse

Name: rsmE [H]

Synonym: Bcen2424_0635

Alternate gene names: 229220796

Gene position: 708294-707527 (Counterclockwise)

Preceding gene: 116688659

Following gene: 116688653

Centisome position: 20.33

GC content: 75.65

Gene sequence:

>768_bases
ATGAACGAAGCCACCACCACCGCGGCCGTGCCGCGCTTTTTCGTCGACGCCGCGCTGCGCGCCGACGCCACGCTCGCGTT
GCCGGCCGACGTCGCGCGTCACGTGCAGGTGCTGCGCCTGCAGCCCGGCGACGTGCTCGCGCTGTTCGACGGCACGGGCG
GCCAGTACCGCGCACGGCTCGTCGAAGTCGACAAGCGCAGCGCGCTCGTGCAGGTCGATACGTTCGAGCCCGCCGAGGCC
GAGCCGCCCTATCGCGTGACGCTCGCGCAGGGGATCGCCGGCGGCGACAAGATGGACTGGGTGATCGAGAAGGCCGTCGA
GCTCGGCGTCGCGGCGGTCGTGCCGCTGTCGACCGCGCGCGGCGTCGTGAAGCTGTCCGGCGAGCGGGCGGACAAGCGGG
TCGCGCACTGGCGCGGCGTCGTGCGCGCGTCGTGCGAGCAGTGCGGACGCAACCGCGTGCCCGACGTCGCGCCGGTCGCC
GGCTTCAACGCGTGGCTCGACGCGCTGCCGGCCGCGCCGGCCGACGGCGAGCTGCGGCTGCTGCTGTCGCCGCGCGCAAG
CAGCCCGTTCGCGTCGCTGCCCGACGCGCCGCCCGCGGCGGCCGTCACGCTGCTGATCGGACCCGAAGGCGGGTTGTCGC
CCGACGAGGAACATGCCGCACGCGCACGCGGGTTCACCGCGCTGTCGCTCGGGCCGCGCGTGCTGCGCACCGAGACGGCC
GGCGCGGCCGTGCTCGCGGCGTTGGCGGCGCGCTGGGGCGGGTGGTAA

Upstream 100 bases:

>100_bases
ATCAGTTCTGGGGCGACCGGTTCGCGCAGATCGAGGATCTCGACGGTTACCGCTGGGCGCTCGGGTGCCGTCTCGACGCA
TGACCGAAACCGCCACACGC

Downstream 100 bases:

>100_bases
AGCGTATCGGCGCGCCGCGTTCAACGGTTCGATGTCGATGACGCCGGAACCCTTGAGCACCAACGCGAACGCACCGATGC
CGGCCGTCTCGAACGAGAAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHVQVLRLQPGDVLALFDGTGGQYRARLVEVDKRSALVQVDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDALPAAPADGELRLLLSPRASSPFASLPDAPPAAAVTLLIGPEGGLSPDEEHAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW

Sequences:

>Translated_255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHVQVLRLQPGDVLALFDGTGGQYRARLVEVDKRSALVQVDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDALPAAPADGELRLLLSPRASSPFASLPDAPPAAAVTLLIGPEGGLSPDEEHAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW
>Mature_255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHVQVLRLQPGDVLALFDGTGGQYRARLVEVDKRSALVQVDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDALPAAPADGELRLLLSPRASSPFASLPDAPPAAAVTLLIGPEGGLSPDEEHAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=245, Percent_Identity=41.2244897959184, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26689; Mature: 26689

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEATTTAAVPRFFVDAALRADATLALPADVARHVQVLRLQPGDVLALFDGTGGQYRARL
CCCCHHHHHHHHHHHHHHHHCCCEEECHHHHHHCEEEEEECCCCEEEEEECCCCCEEEEE
VEVDKRSALVQVDTFEPAEAEPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTAR
EEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCC
GVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVAGFNAWLDALPAAPADGELRL
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LLSPRASSPFASLPDAPPAAAVTLLIGPEGGLSPDEEHAARARGFTALSLGPRVLRTETA
EECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCC
GAAVLAALAARWGGW
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEATTTAAVPRFFVDAALRADATLALPADVARHVQVLRLQPGDVLALFDGTGGQYRARL
CCCCHHHHHHHHHHHHHHHHCCCEEECHHHHHHCEEEEEECCCCEEEEEECCCCCEEEEE
VEVDKRSALVQVDTFEPAEAEPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTAR
EEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCC
GVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVAGFNAWLDALPAAPADGELRL
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LLSPRASSPFASLPDAPPAAAVTLLIGPEGGLSPDEEHAARARGFTALSLGPRVLRTETA
EECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCC
GAAVLAALAARWGGW
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]