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The map label for this gene is appB [H]

Identifier: 227828873

GI number: 227828873

Start: 2467757

End: 2468758

Strand: Direct

Name: appB [H]

Synonym: M1425_2646

Alternate gene names: 227828873

Gene position: 2467757-2468758 (Clockwise)

Preceding gene: 227828871

Following gene: 227828876

Centisome position: 94.59

GC content: 38.32

Gene sequence:

>1002_bases
ATGAATCATAAATGGCTGATTAGGAGACTGGCATCGGTAATTGTTGCGATATTTGCTACAATAGTCATAAGCTGGGCCTT
ATTGGAGTTTTCTCCCTACTCTCCCGCAAATTACATCATGCAATTCATAAATCCACAAGAATTTGCTCAAAAACCTGAAC
TATATAGTTCACTCATAGATTATTTAAACACTCTCAGACCCCATGGGAATCCGTTAATTAGCGCTGCGGATTACATTTGG
AATACGTTACATGGAAATCTGGGCTTCTCGATAATAAGCAACGTACCAGTGGCTCAACTGATTGCAGTTGCGTTACCTTG
GACCTTATTCATCGTAGTAACATCTCTTCTAATAAGTTTCTTCCTTGGGATAAGAATAGGCCAGAAACTAGGGTATATGA
GGGGGACTAAGACCGATTCCATATCTACCGTTTCGCTATCGATCTTAAGATCTGTACCAATTTACATCTATGCCGTATTA
CTTATTTACATACTTGCGTTCACATACCATATCTTCCCCACGGGGGGTGCTTACAGCGTACATGTAACTCCAGGATTTAA
TCTTCCCTTCATAGCTAGCGTGTTGTATCATGCATTTTTACCAATATTCACATTGACCATAGTAAATTTAGTAGGATGGA
TCTTACAGATGAGGGCAAACACAATATATGTACTTGGCGAAGATTTCGTAAACTTTGCTGAAGTCTCTGGAATTAAGAAG
GATATAATAGAAAAGAAGTATATTGGAAAAAATGCAATACTGCCCCTATACACTAGCTTAGTCATAGCAATAGGATTTTC
CTTTAGTGGGTCAGTATTCGTAGAGCAGACATTTTCCTACCCCGGAGTAGGAAACTTACTAATCAACTCCATAACCTCTA
ATGACTATCCCACTGAAATGGGAGTATTCATCATAATAATTGTAGCAGTAATTGTAGGTAATTTAATCGCAGACTTGACA
TATTCGTTCTTAGATCCTAGAGCTAAGGTAGGTGAAGAATAA

Upstream 100 bases:

>100_bases
CGCATGGATATTATTTGTATTTTCCCATTTATAAACTTTGCCTAAACCTTAATGGTATAGTTGAGAAACACTTATATCTT
ATTAAAAGTAATAATAATTT

Downstream 100 bases:

>100_bases
TGGAGGCGTATAAAACGCTATTGGCTATAGGTTATACACTGTTGCTACTTGACGTTTTTTCACTGGTAATCTATAGGAGT
TCCGCAACATTATTTCCTCA

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNHKWLIRRLASVIVAIFATIVISWALLEFSPYSPANYIMQFINPQEFAQKPELYSSLIDYLNTLRPHGNPLISAADYIW
NTLHGNLGFSIISNVPVAQLIAVALPWTLFIVVTSLLISFFLGIRIGQKLGYMRGTKTDSISTVSLSILRSVPIYIYAVL
LIYILAFTYHIFPTGGAYSVHVTPGFNLPFIASVLYHAFLPIFTLTIVNLVGWILQMRANTIYVLGEDFVNFAEVSGIKK
DIIEKKYIGKNAILPLYTSLVIAIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEMGVFIIIIVAVIVGNLIADLT
YSFLDPRAKVGEE

Sequences:

>Translated_333_residues
MNHKWLIRRLASVIVAIFATIVISWALLEFSPYSPANYIMQFINPQEFAQKPELYSSLIDYLNTLRPHGNPLISAADYIW
NTLHGNLGFSIISNVPVAQLIAVALPWTLFIVVTSLLISFFLGIRIGQKLGYMRGTKTDSISTVSLSILRSVPIYIYAVL
LIYILAFTYHIFPTGGAYSVHVTPGFNLPFIASVLYHAFLPIFTLTIVNLVGWILQMRANTIYVLGEDFVNFAEVSGIKK
DIIEKKYIGKNAILPLYTSLVIAIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEMGVFIIIIVAVIVGNLIADLT
YSFLDPRAKVGEE
>Mature_333_residues
MNHKWLIRRLASVIVAIFATIVISWALLEFSPYSPANYIMQFINPQEFAQKPELYSSLIDYLNTLRPHGNPLISAADYIW
NTLHGNLGFSIISNVPVAQLIAVALPWTLFIVVTSLLISFFLGIRIGQKLGYMRGTKTDSISTVSLSILRSVPIYIYAVL
LIYILAFTYHIFPTGGAYSVHVTPGFNLPFIASVLYHAFLPIFTLTIVNLVGWILQMRANTIYVLGEDFVNFAEVSGIKK
DIIEKKYIGKNAILPLYTSLVIAIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEMGVFIIIIVAVIVGNLIADLT
YSFLDPRAKVGEE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=344, Percent_Identity=26.7441860465116, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787053, Length=325, Percent_Identity=27.0769230769231, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789888, Length=329, Percent_Identity=24.9240121580547, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787550, Length=336, Percent_Identity=23.5119047619048, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788504, Length=365, Percent_Identity=24.1095890410959, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787761, Length=269, Percent_Identity=29.7397769516729, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787497, Length=328, Percent_Identity=24.0853658536585, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36994; Mature: 36994

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKWLIRRLASVIVAIFATIVISWALLEFSPYSPANYIMQFINPQEFAQKPELYSSLID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCHHHHHHHHH
YLNTLRPHGNPLISAADYIWNTLHGNLGFSIISNVPVAQLIAVALPWTLFIVVTSLLISF
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLGIRIGQKLGYMRGTKTDSISTVSLSILRSVPIYIYAVLLIYILAFTYHIFPTGGAYSV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HVTPGFNLPFIASVLYHAFLPIFTLTIVNLVGWILQMRANTIYVLGEDFVNFAEVSGIKK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHH
DIIEKKYIGKNAILPLYTSLVIAIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEM
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
GVFIIIIVAVIVGNLIADLTYSFLDPRAKVGEE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MNHKWLIRRLASVIVAIFATIVISWALLEFSPYSPANYIMQFINPQEFAQKPELYSSLID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCHHHHHHHHH
YLNTLRPHGNPLISAADYIWNTLHGNLGFSIISNVPVAQLIAVALPWTLFIVVTSLLISF
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLGIRIGQKLGYMRGTKTDSISTVSLSILRSVPIYIYAVLLIYILAFTYHIFPTGGAYSV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
HVTPGFNLPFIASVLYHAFLPIFTLTIVNLVGWILQMRANTIYVLGEDFVNFAEVSGIKK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHH
DIIEKKYIGKNAILPLYTSLVIAIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEM
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHH
GVFIIIIVAVIVGNLIADLTYSFLDPRAKVGEE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]