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The map label for this gene is ilvI [H]

Identifier: 227828777

GI number: 227828777

Start: 2368452

End: 2370047

Strand: Direct

Name: ilvI [H]

Synonym: M1425_2551

Alternate gene names: 227828777

Gene position: 2368452-2370047 (Clockwise)

Preceding gene: 227828776

Following gene: 227828778

Centisome position: 90.79

GC content: 37.28

Gene sequence:

>1596_bases
ATGGGCAAAACAACATCTGAGCTTCTAATTGACGCAATCTCTTCCCAAGTAACCGACGTATTTGGAATACCAGGAACTCA
TGGTTTATCATTATATGAGGAGCTCAGAAAGAGAGTAAGTAGCGGGGAGATAAGATATTACATGCCTAGATTAGAGTATG
GAGGAGCCATAATGGCAGATTATTATGCTAGATTAAAGGGAAATGTTGGAGTTTTCATGTCAGTTAATGGTCCAGGCTTT
ACTAATTCTTTGACTGGTCTAGTTGAGGCCTATTCTGAAGGTTCACCTCTTGTACTAATCTCTCTAAATAAGGAATTTAA
ATATAGGCATAGGAAGCAACTCCACGATTCTGGGTATTATGACTTACAATTGGAAATGGCCAGACAAGCAACTAAGGCTT
CTTTTAGAATTTACTCCCCAGAAGACGTTCCAATTGTAATGGAAAGAGCTTTTAGAATAGCCCTAGAAGACAAGATGGGG
CCAGTTTACATTGAAGTTCCGGTTGATCTACTAGAAGAGAAGAGTAATATTGAAGATTATAAAGCTAGTAAGAGGATTAA
TAGAACATTAGTCTACCCTACAAAAGAGGAAATAAGGGAAGCATTAAATTTCCTAAGCGAATGCTCAAAACCAATTCTAC
TTTTAGGTTATGGTGCATCAAGATCGAACATCATAAGTTACATTGAGAGGTTGGGAATTCCAGTGTTTACTACCATTAGA
GGGAAAGGGAGTATTCCGGAAAATCATCCTTTATATGCTGGAACAATATTTAACCTTAGTGAAATACCCGGAGATTGCCT
CATAGCAATAGGGACGTCATTTAACGATCTCGAAACTAGTAGATGGAGTATTAAATTGCCGAGAAAGATACTCCACGTGG
ATCCAGACGTAAATGTATTTAACACCTCCTTTAATGCGGAAGTTACCATAAAGGCTAGTGCTGAAGCTTTTCTAGAAGAG
ATAGTTGAGAAGGTTAATTTGCCCAAATGGAGCTATAAAGTAGATGAAAAAACCAATATAGAAAGTAGTAGTAGCGGAAT
AACTCATGACTACTTAGCCAAGGTTTTAGATGAGATGTTAAGTGAGGACAGAGTTATAATTGCTGACGCAGGTACCAATC
AGGTTATGGCAATGGATATAAAAGTATATAGGCCTAATTCGTACTTTAATTCCCTTATCTTTAACGCGATGGGATCAGCC
ATTCCAGCTGGCATAGGAGCCAAAATTGCGTCTCCAGAGAGGCAAATAGTAAGTATTATAGGAGATCTAGGGTTTCAAGG
GTGTTTAAACGAGTTAATTACTGCGGTCCAATATAAGGTTAACTTCTTAACAGTATTAGTAGAGGATGGAGTACAGCACT
TTCTAAGATTAAATCAGAAAATGAGATATGGGAATACCTTTGCAACTGATGTATTTCAAATTGATTACACCAGGGTTATG
GAGGGAATCGGGGTTAATGTAATTGAGGTAAAAGATAAGGAAGATATTAAGAAAAGTGTAGAAGAAGCTGTTGTATTATC
CCTTAAGAGTCCAACAATTCTGAGAGTTCACGTTAATTCTAATAGTATACCTTCTAGATTGTTAATGAAAAGATAG

Upstream 100 bases:

>100_bases
GAATCTTTAAATTATGATTGGGACAAGAGTTCTGAGAAATATTACAATTACTTGGTTCGTAAAACATCAGATTAATTTAT
AGGGATAATAATAGTAATTT

Downstream 100 bases:

>100_bases
CTATTTATAATAAAATAAGGTATTTTACTTATGGTTAAGATACTACTATTTTCTCCAAATCCTAATGAATTTAAGAGAGT
TGGCGTTTATGAGAACAATA

Product: thiamine pyrophosphate protein domain protein TPP-binding

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MGKTTSELLIDAISSQVTDVFGIPGTHGLSLYEELRKRVSSGEIRYYMPRLEYGGAIMADYYARLKGNVGVFMSVNGPGF
TNSLTGLVEAYSEGSPLVLISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIVMERAFRIALEDKMG
PVYIEVPVDLLEEKSNIEDYKASKRINRTLVYPTKEEIREALNFLSECSKPILLLGYGASRSNIISYIERLGIPVFTTIR
GKGSIPENHPLYAGTIFNLSEIPGDCLIAIGTSFNDLETSRWSIKLPRKILHVDPDVNVFNTSFNAEVTIKASAEAFLEE
IVEKVNLPKWSYKVDEKTNIESSSSGITHDYLAKVLDEMLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSA
IPAGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTRVM
EGIGVNVIEVKDKEDIKKSVEEAVVLSLKSPTILRVHVNSNSIPSRLLMKR

Sequences:

>Translated_531_residues
MGKTTSELLIDAISSQVTDVFGIPGTHGLSLYEELRKRVSSGEIRYYMPRLEYGGAIMADYYARLKGNVGVFMSVNGPGF
TNSLTGLVEAYSEGSPLVLISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIVMERAFRIALEDKMG
PVYIEVPVDLLEEKSNIEDYKASKRINRTLVYPTKEEIREALNFLSECSKPILLLGYGASRSNIISYIERLGIPVFTTIR
GKGSIPENHPLYAGTIFNLSEIPGDCLIAIGTSFNDLETSRWSIKLPRKILHVDPDVNVFNTSFNAEVTIKASAEAFLEE
IVEKVNLPKWSYKVDEKTNIESSSSGITHDYLAKVLDEMLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSA
IPAGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTRVM
EGIGVNVIEVKDKEDIKKSVEEAVVLSLKSPTILRVHVNSNSIPSRLLMKR
>Mature_530_residues
GKTTSELLIDAISSQVTDVFGIPGTHGLSLYEELRKRVSSGEIRYYMPRLEYGGAIMADYYARLKGNVGVFMSVNGPGFT
NSLTGLVEAYSEGSPLVLISLNKEFKYRHRKQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIVMERAFRIALEDKMGP
VYIEVPVDLLEEKSNIEDYKASKRINRTLVYPTKEEIREALNFLSECSKPILLLGYGASRSNIISYIERLGIPVFTTIRG
KGSIPENHPLYAGTIFNLSEIPGDCLIAIGTSFNDLETSRWSIKLPRKILHVDPDVNVFNTSFNAEVTIKASAEAFLEEI
VEKVNLPKWSYKVDEKTNIESSSSGITHDYLAKVLDEMLSEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAI
PAGIGAKIASPERQIVSIIGDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTRVME
GIGVNVIEVKDKEDIKKSVEEAVVLSLKSPTILRVHVNSNSIPSRLLMKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=22.9129662522202, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=26.4814814814815, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790104, Length=541, Percent_Identity=25.1386321626617, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1786717, Length=547, Percent_Identity=24.6800731261426, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=22.3230490018149, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788716, Length=462, Percent_Identity=23.8095238095238, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=22.1153846153846, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=528, Percent_Identity=23.4848484848485, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=528, Percent_Identity=23.4848484848485, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=24.2049469964664, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321524, Length=465, Percent_Identity=25.5913978494624, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=469, Percent_Identity=23.6673773987207, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=466, Percent_Identity=23.3905579399142, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320816, Length=530, Percent_Identity=21.3207547169811, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320123, Length=528, Percent_Identity=21.5909090909091, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=22.6252158894646, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59440; Mature: 59309

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTSELLIDAISSQVTDVFGIPGTHGLSLYEELRKRVSSGEIRYYMPRLEYGGAIMAD
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFMSVNGPGFTNSLTGLVEAYSEGSPLVLISLNKEFKYRHRKQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCE
DLQLEMARQATKASFRIYSPEDVPIVMERAFRIALEDKMGPVYIEVPVDLLEEKSNIEDY
EEEEHHHHHHHHCEEEEECCCCCHHHHHHHHHHEEECCCCCEEEEECHHHHHCCCCCHHH
KASKRINRTLVYPTKEEIREALNFLSECSKPILLLGYGASRSNIISYIERLGIPVFTTIR
HHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEC
GKGSIPENHPLYAGTIFNLSEIPGDCLIAIGTSFNDLETSRWSIKLPRKILHVDPDVNVF
CCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEE
NTSFNAEVTIKASAEAFLEEIVEKVNLPKWSYKVDEKTNIESSSSGITHDYLAKVLDEML
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
SEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAIPAGIGAKIASPERQIVSII
CCCCEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
GDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTRVM
HHCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCEEHHEEEEEHHHHH
EGIGVNVIEVKDKEDIKKSVEEAVVLSLKSPTILRVHVNSNSIPSRLLMKR
HHCCCEEEEECCHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHCCC
>Mature Secondary Structure 
GKTTSELLIDAISSQVTDVFGIPGTHGLSLYEELRKRVSSGEIRYYMPRLEYGGAIMAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFMSVNGPGFTNSLTGLVEAYSEGSPLVLISLNKEFKYRHRKQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCE
DLQLEMARQATKASFRIYSPEDVPIVMERAFRIALEDKMGPVYIEVPVDLLEEKSNIEDY
EEEEHHHHHHHHCEEEEECCCCCHHHHHHHHHHEEECCCCCEEEEECHHHHHCCCCCHHH
KASKRINRTLVYPTKEEIREALNFLSECSKPILLLGYGASRSNIISYIERLGIPVFTTIR
HHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEC
GKGSIPENHPLYAGTIFNLSEIPGDCLIAIGTSFNDLETSRWSIKLPRKILHVDPDVNVF
CCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEE
NTSFNAEVTIKASAEAFLEEIVEKVNLPKWSYKVDEKTNIESSSSGITHDYLAKVLDEML
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
SEDRVIIADAGTNQVMAMDIKVYRPNSYFNSLIFNAMGSAIPAGIGAKIASPERQIVSII
CCCCEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
GDLGFQGCLNELITAVQYKVNFLTVLVEDGVQHFLRLNQKMRYGNTFATDVFQIDYTRVM
HHCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCEEHHEEEEEHHHHH
EGIGVNVIEVKDKEDIKKSVEEAVVLSLKSPTILRVHVNSNSIPSRLLMKR
HHCCCEEEEECCHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]