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The map label for this gene is hycF [C]

Identifier: 227828751

GI number: 227828751

Start: 2338339

End: 2339820

Strand: Direct

Name: hycF [C]

Synonym: M1425_2513

Alternate gene names: 227828751

Gene position: 2338339-2339820 (Clockwise)

Preceding gene: 227828750

Following gene: 227828752

Centisome position: 89.63

GC content: 37.92

Gene sequence:

>1482_bases
ATGTTAAACGCTGGATTAATAGTATCGAAAAAAGCTAGGGAGATAATAGGGGATGAGATACTAAAGGAGGTTTTTGAAGA
GGCTAAACTATCTTATGTTGCTGAGATGGGTGATTACATAATAGATGACGTTAAGAATAATGAGCTAAAAGCTTTGTTAG
TAGTTAGCGAAAATGGAAGAGAAAGATGGATGGAGGATATAGATCAGAAGTTGGGGATTTCGCCATTAGCTATTCTTATA
ATACCTCCGACCTGGTTTAAAGATAAGAGTAAGAAGTACGTTTTTACCTTACTTATGGCATATTCTTTGAGAATAGAATT
AATGGATTTAGCTTATAGAGTTCAACCTACTGCCACTTCCTCGGTCTCCAGAAGATCATTGCTTAAATTGAAAACATACG
AATACAAACCATACCCAGCATTATTCGATGAAGTTCACGCAGAAAGGGAAATAAATAGAGCGATTGAGTCTTGCCCTCAA
GGGTTAATAGTTAAAGCTCCAGAAGGTCCATCAGTGGGTTATCCAGAGAGATGCAGCGTATGTGGTTATTGCTCCGCGAG
TTCATACTTAGGGTATCTTGAAATTCCAACAGCGACTACGGATCAAGTAGTGAGTTTCATTAATGTAATAGTTAGATACT
ATGAGGATAAACAAGCTGCATTATTGTTTACAGACTCAATAATAGACGAGGTACCAGAAGGCATATTCCCATTCTTAATG
CCTTGTACTGCAGGAGTTCACGACTCCTTCGTTCTTGCATCATATGCCGCTGGAATAACTCCTATAGTTCACGTTTCATC
AAAATGTGGAAGTAGGGATATTGCGTTAAAGAGATTAGACGAATTACCATCTCATTTTCCCGGTACTAGTTTCACCATTA
GTAAGGCTAAAGATGACGAGGAGCTTAAAAGGACACTCCTCTCTATTAAGCTGACACAACTTAGTAGATCCGAAATACCC
CTAGACGTTATTTTACAGCGTAGTAGGAGAAGAGCGCTCCTAATATGGTCCATAGAAGAAATGTCGAAAAAGGTTAAATT
AAACGAAGATGATGTTGTCCCAGAAGTTTATAACGTAGAGGTGGATCCAAACAAGTGCGTATTATGTGGAGTATGTGTAA
GGGCTTGTCAAATGTTAGTTCCCGAGTTAAAGGGTAATAATACTTTAGAGTTAAGCTATAATATACCTTATTGTATTGGT
TCACAACGATGTGTTAGGAATTGCCCAGAGAAAGCCGTAGTAGTAACTGGTTTCGCCAAGATTTCTGACCTTAAAAAGAA
AGTTGTAAATAAGGCAGAGGTGGCTAAGTGTAGATTCTGTGGAAAACCATTGGGTTCAGAGAAGATCAAGACTAAGGTTG
ATACTCTACTAATACAATATGGATTCGCTGGAACTGCACAGTATACTGATATCTGCAATGAATGTAAGCAAAAAATATTA
ACTAAGATCTGGTTGGAGAAATTGTTGAGTGGTAAGAAATGA

Upstream 100 bases:

>100_bases
ATGACGTAATTAACGATCCGAGATCAAATATATACAAAATTGTATGTTCTGAATTGGCTAAGTTTCTTCAATTTGAGAGA
AAATATTTAGGTGTAAATTA

Downstream 100 bases:

>100_bases
TACCATACATTATTGACTTAGATGCAATCTTCGATCTATCCTCTATTGAAATCTATAGACTAATCACTTCATCCCTCTCA
AATGTAAGATACTATCCACC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: None

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYIIDDVKNNELKALLVVSENGRERWMEDIDQKLGISPLAILI
IPPTWFKDKSKKYVFTLLMAYSLRIELMDLAYRVQPTATSSVSRRSLLKLKTYEYKPYPALFDEVHAEREINRAIESCPQ
GLIVKAPEGPSVGYPERCSVCGYCSASSYLGYLEIPTATTDQVVSFINVIVRYYEDKQAALLFTDSIIDEVPEGIFPFLM
PCTAGVHDSFVLASYAAGITPIVHVSSKCGSRDIALKRLDELPSHFPGTSFTISKAKDDEELKRTLLSIKLTQLSRSEIP
LDVILQRSRRRALLIWSIEEMSKKVKLNEDDVVPEVYNVEVDPNKCVLCGVCVRACQMLVPELKGNNTLELSYNIPYCIG
SQRCVRNCPEKAVVVTGFAKISDLKKKVVNKAEVAKCRFCGKPLGSEKIKTKVDTLLIQYGFAGTAQYTDICNECKQKIL
TKIWLEKLLSGKK

Sequences:

>Translated_493_residues
MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYIIDDVKNNELKALLVVSENGRERWMEDIDQKLGISPLAILI
IPPTWFKDKSKKYVFTLLMAYSLRIELMDLAYRVQPTATSSVSRRSLLKLKTYEYKPYPALFDEVHAEREINRAIESCPQ
GLIVKAPEGPSVGYPERCSVCGYCSASSYLGYLEIPTATTDQVVSFINVIVRYYEDKQAALLFTDSIIDEVPEGIFPFLM
PCTAGVHDSFVLASYAAGITPIVHVSSKCGSRDIALKRLDELPSHFPGTSFTISKAKDDEELKRTLLSIKLTQLSRSEIP
LDVILQRSRRRALLIWSIEEMSKKVKLNEDDVVPEVYNVEVDPNKCVLCGVCVRACQMLVPELKGNNTLELSYNIPYCIG
SQRCVRNCPEKAVVVTGFAKISDLKKKVVNKAEVAKCRFCGKPLGSEKIKTKVDTLLIQYGFAGTAQYTDICNECKQKIL
TKIWLEKLLSGKK
>Mature_493_residues
MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYIIDDVKNNELKALLVVSENGRERWMEDIDQKLGISPLAILI
IPPTWFKDKSKKYVFTLLMAYSLRIELMDLAYRVQPTATSSVSRRSLLKLKTYEYKPYPALFDEVHAEREINRAIESCPQ
GLIVKAPEGPSVGYPERCSVCGYCSASSYLGYLEIPTATTDQVVSFINVIVRYYEDKQAALLFTDSIIDEVPEGIFPFLM
PCTAGVHDSFVLASYAAGITPIVHVSSKCGSRDIALKRLDELPSHFPGTSFTISKAKDDEELKRTLLSIKLTQLSRSEIP
LDVILQRSRRRALLIWSIEEMSKKVKLNEDDVVPEVYNVEVDPNKCVLCGVCVRACQMLVPELKGNNTLELSYNIPYCIG
SQRCVRNCPEKAVVVTGFAKISDLKKKVVNKAEVAKCRFCGKPLGSEKIKTKVDTLLIQYGFAGTAQYTDICNECKQKIL
TKIWLEKLLSGKK

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 55302; Mature: 55302

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYIIDDVKNNELKALLVVSENGR
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
ERWMEDIDQKLGISPLAILIIPPTWFKDKSKKYVFTLLMAYSLRIELMDLAYRVQPTATS
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHEECCCCHH
SVSRRSLLKLKTYEYKPYPALFDEVHAEREINRAIESCPQGLIVKAPEGPSVGYPERCSV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCE
CGYCSASSYLGYLEIPTATTDQVVSFINVIVRYYEDKQAALLFTDSIIDEVPEGIFPFLM
ECCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHH
PCTAGVHDSFVLASYAAGITPIVHVSSKCGSRDIALKRLDELPSHFPGTSFTISKAKDDE
HHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHH
ELKRTLLSIKLTQLSRSEIPLDVILQRSRRRALLIWSIEEMSKKVKLNEDDVVPEVYNVE
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCEEEEE
VDPNKCVLCGVCVRACQMLVPELKGNNTLELSYNIPYCIGSQRCVRNCPEKAVVVTGFAK
ECCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCHHHHCCCCCEEEEECHHH
ISDLKKKVVNKAEVAKCRFCGKPLGSEKIKTKVDTLLIQYGFAGTAQYTDICNECKQKIL
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TKIWLEKLLSGKK
HHHHHHHHHCCCC
>Mature Secondary Structure
MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYIIDDVKNNELKALLVVSENGR
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
ERWMEDIDQKLGISPLAILIIPPTWFKDKSKKYVFTLLMAYSLRIELMDLAYRVQPTATS
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHEECCCCHH
SVSRRSLLKLKTYEYKPYPALFDEVHAEREINRAIESCPQGLIVKAPEGPSVGYPERCSV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCE
CGYCSASSYLGYLEIPTATTDQVVSFINVIVRYYEDKQAALLFTDSIIDEVPEGIFPFLM
ECCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHH
PCTAGVHDSFVLASYAAGITPIVHVSSKCGSRDIALKRLDELPSHFPGTSFTISKAKDDE
HHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHH
ELKRTLLSIKLTQLSRSEIPLDVILQRSRRRALLIWSIEEMSKKVKLNEDDVVPEVYNVE
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCEEEEE
VDPNKCVLCGVCVRACQMLVPELKGNNTLELSYNIPYCIGSQRCVRNCPEKAVVVTGFAK
ECCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCHHHHCCCCCEEEEECHHH
ISDLKKKVVNKAEVAKCRFCGKPLGSEKIKTKVDTLLIQYGFAGTAQYTDICNECKQKIL
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TKIWLEKLLSGKK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA