Click here to switch to the map view.

The map label for this gene is nrfC [H]

Identifier: 227828749

GI number: 227828749

Start: 2336825

End: 2337667

Strand: Direct

Name: nrfC [H]

Synonym: M1425_2511

Alternate gene names: 227828749

Gene position: 2336825-2337667 (Clockwise)

Preceding gene: 227828748

Following gene: 227828750

Centisome position: 89.57

GC content: 42.23

Gene sequence:

>843_bases
ATGTCTTCTCCCCAAAATACGACCACACCCCAAATTAAATTAGATATAAGACAAGGTGGGGTTCAGCATGCTCCACCGTT
CAGCTTAAAGGCAAACTACGCCATAATAACTGACCTAAACAAGTGTTTCGGATGTGGAGGCTGCCAAATGTCATGTAAGG
AATGGAACACATCTGGAATGTTTGGACCAATACCAGATCTAAATCCGTACGGAGAATTAGACGTTATGTTCTGGTTAAGA
GTACTATATGTAGAAGTGGGGACATACCCCCAGACTAAGGTTTACAATATACCAATAAACTGTTTCCACTGTGTAAGTGC
GCCTTGTACTGAAGTTTGCCCAGTAGGCGCTACATTCAAGAGGACTGAAGATGGTATAGTTTTAGTTGACTATAACGAAT
GCATAGGGACTAAGTACTGTATTTACGCATGTCCATATGGGAACAGATTCTTCGATTATGTAGAGGGAGTTACGAAGAAG
TGCACCCATTGTTTCGATAGGATTTATGATCCTACGTTACCTCCAGAAGAGAGAATACCAGCGTGTATTCACGGGTGCAT
GGTTCAAGCGAGAATTTGGGCTAATATCTTAGACCCAACTGATCCTGGTACAATCCTGTTCTACGATAAAGGAGGATTTG
TAATGGGGCCTGAAACTGGAGCACAGCCTGCTAGTGGCTATTTACCATGGCGTAGCAAATACGCTGAAAGTGATGTCCAA
TTACTTAGTGAGGCTGAGTACTATAACGTATGGACCGCTAACGGCGTTGTACAACTTGGAGCTCAAGGGAACAACTCTAC
ATATACTGAAGATAATGGCTCAAATTCTAATTCAAGTTCATAA

Upstream 100 bases:

>100_bases
TATGTTGCACCTGGTTCGTTAGCCCCAACTTATACTATAGGGCAATATAAGATAAGATATGGGTTCGCAACAGAGCCCTC
TTCCTCTTAAGGTGATAAAT

Downstream 100 bases:

>100_bases
TTTTTTGTTTGGTGACTTATATTGTCAGTTTCCGTCTTGTTAAACGTTAGGCATGCGTTATACGATTTATTCGCGGATCT
CTTCCTTTATAAGTTTGTAG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGMFGPIPDLNPYGELDVMFWLR
VLYVEVGTYPQTKVYNIPINCFHCVSAPCTEVCPVGATFKRTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKK
CTHCFDRIYDPTLPPEERIPACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQ
LLSEAEYYNVWTANGVVQLGAQGNNSTYTEDNGSNSNSSS

Sequences:

>Translated_280_residues
MSSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGMFGPIPDLNPYGELDVMFWLR
VLYVEVGTYPQTKVYNIPINCFHCVSAPCTEVCPVGATFKRTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKK
CTHCFDRIYDPTLPPEERIPACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQ
LLSEAEYYNVWTANGVVQLGAQGNNSTYTEDNGSNSNSSS
>Mature_279_residues
SSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGMFGPIPDLNPYGELDVMFWLRV
LYVEVGTYPQTKVYNIPINCFHCVSAPCTEVCPVGATFKRTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKKC
THCFDRIYDPTLPPEERIPACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQL
LSEAEYYNVWTANGVVQLGAQGNNSTYTEDNGSNSNSSS

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=206, Percent_Identity=31.0679611650485, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=221, Percent_Identity=30.316742081448, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787872, Length=216, Percent_Identity=28.7037037037037, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI226510944, Length=171, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787740, Length=82, Percent_Identity=41.4634146341463, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787478, Length=160, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=161, Percent_Identity=29.8136645962733, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790326, Length=162, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789370, Length=195, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082180, Length=244, Percent_Identity=24.5901639344262, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI2367245, Length=163, Percent_Identity=28.8343558282209, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 30991; Mature: 30860

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGM
CCCCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEEECHHHHCCCCCCCCCCCCCCCCC
FGPIPDLNPYGELDVMFWLRVLYVEVGTYPQTKVYNIPINCFHCVSAPCTEVCPVGATFK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHCCCCCCEE
RTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKKCTHCFDRIYDPTLPPEERIP
ECCCCEEEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCH
ACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQ
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
LLSEAEYYNVWTANGVVQLGAQGNNSTYTEDNGSNSNSSS
HHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGM
CCCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEEECHHHHCCCCCCCCCCCCCCCCC
FGPIPDLNPYGELDVMFWLRVLYVEVGTYPQTKVYNIPINCFHCVSAPCTEVCPVGATFK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHCCCCCCEE
RTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKKCTHCFDRIYDPTLPPEERIP
ECCCCEEEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCH
ACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQ
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
LLSEAEYYNVWTANGVVQLGAQGNNSTYTEDNGSNSNSSS
HHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]