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The map label for this gene is proP [H]

Identifier: 227828694

GI number: 227828694

Start: 2275328

End: 2276671

Strand: Direct

Name: proP [H]

Synonym: M1425_2452

Alternate gene names: 227828694

Gene position: 2275328-2276671 (Clockwise)

Preceding gene: 227828692

Following gene: 227828698

Centisome position: 87.22

GC content: 38.1

Gene sequence:

>1344_bases
ATGGAGCCAGAAAAGTTCACGTGGGATTCAGGTAAGGCAATAATATCCCAATTTATAGGATTCCTTCTAGATTCCTATGA
TTTGACAATGATTTTAAGCATAGCTCCAGTGTTAGCTAAGGTAATTCTACCACCTGCAAGCCCACTAATTGCGACATTTA
ATATCATTCTTTCCTACTCCCTAACAATAATATTCAGACCCTTAGGTTCAGCAATATTTGGAAATTTAGGAGATAAGATA
GGTAGGAGAGCTGACTTAATAATTACGGTATTGGGTTTAGGAATTACTAGTGCACTTACTTCGGCATTGCCAACTTATGC
CCAAGCTGGAATTCTCTCATTCGCTCTATTCGTTTTAATAAGAGTTGCGGTAGGCATTTTCGCTGGTGGAGAATACTCAG
CTGGTCATCCATTTGCTATGGAATGGACACCATATAAATGGAGAGGGTTTGTTAGTGGATTAGTTCAAGGTGGTTTCTCA
TTTGGTGCAGCCTTGGCTGCTGTGGTAGAGGGCATCTTCATAGGAATTTATGGTGTATCTGGTGTTGTGGAGTTTGCGTG
GAGATATGTGTTTTTAACTGCTGTAATACCAGCTTTCATAGCCTTAGCAGTAAGACTCGCTATGAAAGAAACACCAGTTT
TTGAAGATGTCAAAAAGAAGAACCTAATCAGAAGGACACCATTAGTAGATTTGTTTAAACCACCTTATAGAAGAGATTTC
TTTCAAGTCATGGCATATATGACGGGGATGTTCTTCTTCGCTTATTCTCTATTTGCATTTGTACCTACTATTTTAGAACA
TAGTCCATCAATATTTAGCCTTGGAGAGGCAAACTACATATATAGCATCGGTACTTACGCTGCATTCGCTGGTGCAGTAA
TGTTCGGTACCTTATCACAGTATGTTGGAAGAAGAAGGTTAACAATAATATGGAGTATAATCACCTTTATCATTGCAATA
CCAATTTATTATGGGCTTATTACTGCAGCAATTGCGGGTAACGCTATAACTGCTATGTTATTTTCAATTATCATCGGTAT
AATAACACAAGGACCTTGGGGAATAATACCCATCTACTTATCTGAAAGATTTAAAGCATCAATGAGGGCTTCTGGAGTGG
GTTTCGGCTATTCCTCTGGAATTTTCATAGGTGGTTGGTTCAGTATATATGTGCCTCTAATGCATAACTACTTGTTTAAG
TCATTAGATACACCGAATAACATATGGTTCTCTACGGCAGTATTATTGATGATTGGAGCTTTAATAGTTGGTATTGCACA
ATACTTCGGACCAGAAACACTAGGAACTAAATTGGCTGAAGAACCACAAAAAACCATAGAATAA

Upstream 100 bases:

>100_bases
ATGCGAGGTATAAAATGTTGAGCATTGTATACTGTTTAAATAACTTAAATTCTTTTCTAAGAAGTTAATTTTTAAAACAA
CAGTTTAAAGAAGAGCTCAA

Downstream 100 bases:

>100_bases
AATGGAAAAGTACAGCTTTTTAAAAAGGATTTTAATTTTTTTCAAAAACTTTTATTTTATCTTTTTTTACATACACTAAT
ACCTCCTCACCAGAATGTAT

Product: major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFALFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFVSGLVQGGFS
FGAALAAVVEGIFIGIYGVSGVVEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLVDLFKPPYRRDF
FQVMAYMTGMFFFAYSLFAFVPTILEHSPSIFSLGEANYIYSIGTYAAFAGAVMFGTLSQYVGRRRLTIIWSIITFIIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFIGGWFSIYVPLMHNYLFK
SLDTPNNIWFSTAVLLMIGALIVGIAQYFGPETLGTKLAEEPQKTIE

Sequences:

>Translated_447_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFALFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFVSGLVQGGFS
FGAALAAVVEGIFIGIYGVSGVVEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLVDLFKPPYRRDF
FQVMAYMTGMFFFAYSLFAFVPTILEHSPSIFSLGEANYIYSIGTYAAFAGAVMFGTLSQYVGRRRLTIIWSIITFIIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFIGGWFSIYVPLMHNYLFK
SLDTPNNIWFSTAVLLMIGALIVGIAQYFGPETLGTKLAEEPQKTIE
>Mature_447_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFALFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFVSGLVQGGFS
FGAALAAVVEGIFIGIYGVSGVVEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLVDLFKPPYRRDF
FQVMAYMTGMFFFAYSLFAFVPTILEHSPSIFSLGEANYIYSIGTYAAFAGAVMFGTLSQYVGRRRLTIIWSIITFIIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFIGGWFSIYVPLMHNYLFK
SLDTPNNIWFSTAVLLMIGALIVGIAQYFGPETLGTKLAEEPQKTIE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=383, Percent_Identity=27.9373368146214, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=27.1428571428571, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789941, Length=406, Percent_Identity=26.1083743842365, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=21.832884097035, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082231, Length=493, Percent_Identity=21.9066937119675, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=23.9583333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786229, Length=466, Percent_Identity=25.1072961373391, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322632, Length=402, Percent_Identity=25.6218905472637, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48881; Mature: 48881

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCHHHHHHHHHCHHHHCC
>Mature Secondary Structure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HHHHHHHHHCCHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]