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The map label for this gene is gsiB [H]

Identifier: 227828577

GI number: 227828577

Start: 2148294

End: 2150444

Strand: Direct

Name: gsiB [H]

Synonym: M1425_2331

Alternate gene names: 227828577

Gene position: 2148294-2150444 (Clockwise)

Preceding gene: 227828576

Following gene: 227828580

Centisome position: 82.35

GC content: 38.26

Gene sequence:

>2151_bases
ATGAAGTTCTTAAGAAAATTAATATCAATAATTGTAGCTGTTTTAATGATAATAAGTTTAGGGATAGCATTCAATATTGT
AAGATCACAACAAATGTCAATGCAACCGGAAGGGAGTTTGGTTGTAGTCGCTAATACTATTGGTACATGGCAAGATAACT
TTAACCCATGGAACTCTCCATATTCTTCCTATGATGGGGAGTGGTTTATTTATGAGCCGCTAGCTCTGATAGACTACGGT
ACTTCACAAATTATTCCATGGCTGGCTACTAATTGGACATTCACAAATACTACTACATATGTCAATGACAAACCGGCACC
AACATTTGGAATAGTCTTGTGGTTGAGGCATGGTGTGTACTTCACAGATGGCACACCATTCAATGCAACAGCAGTATGGT
ACACGTTCGCCCTAGAACAAGCATACCCACAACTCGGCTATACTGCAAACGACATAGCAAACATGACAATAATAAACCCA
TATGAAATAGAAATAGTATTTAATCAAAGAGCAACTCCTGCTATTTTATATACAATCCTAGGCCATTTTATCGTCGATCC
TGCACAGTGGGGTAAAATATTCCCAGTAGAGCAATTACCAAATGGTACATATGTAGCCTTAAACAAGACTGGTAATCCAT
ACACTTACCCCAATCCAAATCCCATAGGCACTGGGCCATACATGCTATACAGCTTCTCAGCACAACAAATAGTACTAGTG
GCCAACCCACACTATTGGATGCCAGGAGAACCAAGAATAAAATACCTACTATTCCCAGCTTACGCAAGTAGTTCACAAGC
TGCAACCGATTTATCTCTAGGTAAAATACAATGGGCAGGATTATCTATTCAAGATATTCAGTCTGTATTCGTAGCCAAAA
ACCCGCAATACAACCATTACTTTTTCCAACACGTTACATCACCTGGCGTTTTATATCTCAATGACATGAGATGGCCTTTA
TCTGATCCAATTTTAAGACAAGCTATTAGTCTTGCAATTAATAGAACTGCCATAACATATCTAGTATACTATAATTACAC
AACACCAGCTATAGATTTACCAGTTTCGGTAGGCCAGCTCGGGTTTTTTAACTCAACTGTAATACAACTAGCTCAAGAGT
TAGCTCCCCCACAAGGCAATGTAACTGCAGCGTTACAGTTGCTTGAATCCCACGGTTATAAACTAATTAATGGGCAATTG
ATAGCACCGAATGGTACTCCAGTTCCTACAATGACTATTGAAGCGCCTGCGGGTTCGACAGTATGGGATGCGGAAGTAAA
CCTTTATGCTCAACAATTGAAACAAATAGGGCTAAACGTTCAAGTAGTAATTCCTCCATTTTCCACTGCGTTAAATGATT
TACTGAGTGGGAATTTCTGGATGGAAAGAATGTATCTGCTATCAACTGGTCCTACACCAATAATGTTTTTCCAAGCCTTT
CTCTATAATCCGGTGAATACACCAGGTAATATAACGCCAATAGGTAATAGTACAATATACAACGTAGGTAGGCTAAACTT
ATCTTTGCGTCTATCTCCCAACTCTCCACCGATAATTAAACTTTATCAATATGCAATGGGTAACATAAGTAATGTGGCTG
AGTATGAGAAGCTTTTAAATGAGATTGGAATGTTCTGGCTGAAATATATGCCAGCAATTGTCCTAATAAACGGATGGGTG
AATTACGAATATATTAATAGTTCGATTTCTGGATGGGTAACTCCACAACATAACTATTGGATTGGAGCACCATGGTATAA
CCCACCTTTACCCGTTATTTTATCATTACACCTAGCTAATCAGTCTGTTCCAGAGCCTTGGTGGTATTATGAAAGTCAGT
TTAATATTACAGTTCCTTCCAGTTGGTTGACTTCAAATGACCCATTCGTATACTCTACAAGTACTACTACGACTAGTACA
AGTACTAGGACAACTAGTGCTACTACGTCGACAACTACATCTACTAGTACTGTTATTACTACGAGTGTTTCGACTACTAC
TAGTGTTTCAACATCTGTGAGTACTACTACTAGTACTGTAACTAGCACTGTTACCAGCTCATCATCCAATACTACACTAT
ATGTCATAATAGCAGTAGTAGTTATTATCATAGTAATAATAGGAGTAGTACTAGGACTAAGAAGAAGATAA

Upstream 100 bases:

>100_bases
TTACCCGTTAGGGATATAGAATCTTTAATTTAAATTATAATCTAACTAGTAACTTGTAAAAATTAAATATGTATAAAGCG
TTAAGATATCTGTGGATATA

Downstream 100 bases:

>100_bases
AAACAATAATAATTATGAAGAAATTTTATTTTTTAAAACTTCTTTACATTTAGCTTTAGAAATTAATGATAGATAAAAAG
CTATTCGCTTCTTCGATTAC

Product: extracellular solute-binding protein family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MKFLRKLISIIVAVLMIISLGIAFNIVRSQQMSMQPEGSLVVVANTIGTWQDNFNPWNSPYSSYDGEWFIYEPLALIDYG
TSQIIPWLATNWTFTNTTTYVNDKPAPTFGIVLWLRHGVYFTDGTPFNATAVWYTFALEQAYPQLGYTANDIANMTIINP
YEIEIVFNQRATPAILYTILGHFIVDPAQWGKIFPVEQLPNGTYVALNKTGNPYTYPNPNPIGTGPYMLYSFSAQQIVLV
ANPHYWMPGEPRIKYLLFPAYASSSQAATDLSLGKIQWAGLSIQDIQSVFVAKNPQYNHYFFQHVTSPGVLYLNDMRWPL
SDPILRQAISLAINRTAITYLVYYNYTTPAIDLPVSVGQLGFFNSTVIQLAQELAPPQGNVTAALQLLESHGYKLINGQL
IAPNGTPVPTMTIEAPAGSTVWDAEVNLYAQQLKQIGLNVQVVIPPFSTALNDLLSGNFWMERMYLLSTGPTPIMFFQAF
LYNPVNTPGNITPIGNSTIYNVGRLNLSLRLSPNSPPIIKLYQYAMGNISNVAEYEKLLNEIGMFWLKYMPAIVLINGWV
NYEYINSSISGWVTPQHNYWIGAPWYNPPLPVILSLHLANQSVPEPWWYYESQFNITVPSSWLTSNDPFVYSTSTTTTST
STRTTSATTSTTTSTSTVITTSVSTTTSVSTSVSTTTSTVTSTVTSSSSNTTLYVIIAVVVIIIVIIGVVLGLRRR

Sequences:

>Translated_716_residues
MKFLRKLISIIVAVLMIISLGIAFNIVRSQQMSMQPEGSLVVVANTIGTWQDNFNPWNSPYSSYDGEWFIYEPLALIDYG
TSQIIPWLATNWTFTNTTTYVNDKPAPTFGIVLWLRHGVYFTDGTPFNATAVWYTFALEQAYPQLGYTANDIANMTIINP
YEIEIVFNQRATPAILYTILGHFIVDPAQWGKIFPVEQLPNGTYVALNKTGNPYTYPNPNPIGTGPYMLYSFSAQQIVLV
ANPHYWMPGEPRIKYLLFPAYASSSQAATDLSLGKIQWAGLSIQDIQSVFVAKNPQYNHYFFQHVTSPGVLYLNDMRWPL
SDPILRQAISLAINRTAITYLVYYNYTTPAIDLPVSVGQLGFFNSTVIQLAQELAPPQGNVTAALQLLESHGYKLINGQL
IAPNGTPVPTMTIEAPAGSTVWDAEVNLYAQQLKQIGLNVQVVIPPFSTALNDLLSGNFWMERMYLLSTGPTPIMFFQAF
LYNPVNTPGNITPIGNSTIYNVGRLNLSLRLSPNSPPIIKLYQYAMGNISNVAEYEKLLNEIGMFWLKYMPAIVLINGWV
NYEYINSSISGWVTPQHNYWIGAPWYNPPLPVILSLHLANQSVPEPWWYYESQFNITVPSSWLTSNDPFVYSTSTTTTST
STRTTSATTSTTTSTSTVITTSVSTTTSVSTSVSTTTSTVTSTVTSSSSNTTLYVIIAVVVIIIVIIGVVLGLRRR
>Mature_716_residues
MKFLRKLISIIVAVLMIISLGIAFNIVRSQQMSMQPEGSLVVVANTIGTWQDNFNPWNSPYSSYDGEWFIYEPLALIDYG
TSQIIPWLATNWTFTNTTTYVNDKPAPTFGIVLWLRHGVYFTDGTPFNATAVWYTFALEQAYPQLGYTANDIANMTIINP
YEIEIVFNQRATPAILYTILGHFIVDPAQWGKIFPVEQLPNGTYVALNKTGNPYTYPNPNPIGTGPYMLYSFSAQQIVLV
ANPHYWMPGEPRIKYLLFPAYASSSQAATDLSLGKIQWAGLSIQDIQSVFVAKNPQYNHYFFQHVTSPGVLYLNDMRWPL
SDPILRQAISLAINRTAITYLVYYNYTTPAIDLPVSVGQLGFFNSTVIQLAQELAPPQGNVTAALQLLESHGYKLINGQL
IAPNGTPVPTMTIEAPAGSTVWDAEVNLYAQQLKQIGLNVQVVIPPFSTALNDLLSGNFWMERMYLLSTGPTPIMFFQAF
LYNPVNTPGNITPIGNSTIYNVGRLNLSLRLSPNSPPIIKLYQYAMGNISNVAEYEKLLNEIGMFWLKYMPAIVLINGWV
NYEYINSSISGWVTPQHNYWIGAPWYNPPLPVILSLHLANQSVPEPWWYYESQFNITVPSSWLTSNDPFVYSTSTTTTST
STRTTSATTSTTTSTSTVITTSVSTTTSVSTSVSTTTSTVTSTVTSSSSNTTLYVIIAVVVIIIVIIGVVLGLRRR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=342, Percent_Identity=26.9005847953216, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 79590; Mature: 79590

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLRKLISIIVAVLMIISLGIAFNIVRSQQMSMQPEGSLVVVANTIGTWQDNFNPWNSP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
YSSYDGEWFIYEPLALIDYGTSQIIPWLATNWTFTNTTTYVNDKPAPTFGIVLWLRHGVY
CCCCCCCEEEECCEEEEECCCCCCCCEEECCEEEECEEEEECCCCCCHHHHHEEHHCCEE
FTDGTPFNATAVWYTFALEQAYPQLGYTANDIANMTIINPYEIEIVFNQRATPAILYTIL
EECCCCCCCEEEEEEEEHHHHCCCCCCCHHHCCCEEEECCEEEEEEECCCCCHHHHHHHH
GHFIVDPAQWGKIFPVEQLPNGTYVALNKTGNPYTYPNPNPIGTGPYMLYSFSAQQIVLV
HHHHCCHHHCCCEEEHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLFPAYASSSQAATDLSLGKIQWAGLSIQDIQSVFVAKNPQYNHY
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEECCCCCCCE
FFQHVTSPGVLYLNDMRWPLSDPILRQAISLAINRTAITYLVYYNYTTPAIDLPVSVGQL
EEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCEECCCCCCCC
GFFNSTVIQLAQELAPPQGNVTAALQLLESHGYKLINGQLIAPNGTPVPTMTIEAPAGST
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEECCCCCE
VWDAEVNLYAQQLKQIGLNVQVVIPPFSTALNDLLSGNFWMERMYLLSTGPTPIMFFQAF
EEECHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
LYNPVNTPGNITPIGNSTIYNVGRLNLSLRLSPNSPPIIKLYQYAMGNISNVAEYEKLLN
HCCCCCCCCCEEECCCCEEEEEEEEEEEEEECCCCCCEEEEHHHHCCCCCHHHHHHHHHH
EIGMFWLKYMPAIVLINGWVNYEYINSSISGWVTPQHNYWIGAPWYNPPLPVILSLHLAN
HHHHHHHHHCCEEEEEECCEEEEEECCCCCEEECCCCCEEEECCCCCCCHHHEEEEEECC
QSVPEPWWYYESQFNITVPSSWLTSNDPFVYSTSTTTTSTSTRTTSATTSTTTSTSTVIT
CCCCCCCEEEECEEEEECCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCCCCEEEE
TSVSTTTSVSTSVSTTTSTVTSTVTSSSSNTTLYVIIAVVVIIIVIIGVVLGLRRR
EECCCCCCCCCCCCCCHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFLRKLISIIVAVLMIISLGIAFNIVRSQQMSMQPEGSLVVVANTIGTWQDNFNPWNSP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
YSSYDGEWFIYEPLALIDYGTSQIIPWLATNWTFTNTTTYVNDKPAPTFGIVLWLRHGVY
CCCCCCCEEEECCEEEEECCCCCCCCEEECCEEEECEEEEECCCCCCHHHHHEEHHCCEE
FTDGTPFNATAVWYTFALEQAYPQLGYTANDIANMTIINPYEIEIVFNQRATPAILYTIL
EECCCCCCCEEEEEEEEHHHHCCCCCCCHHHCCCEEEECCEEEEEEECCCCCHHHHHHHH
GHFIVDPAQWGKIFPVEQLPNGTYVALNKTGNPYTYPNPNPIGTGPYMLYSFSAQQIVLV
HHHHCCHHHCCCEEEHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLFPAYASSSQAATDLSLGKIQWAGLSIQDIQSVFVAKNPQYNHY
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEECCCCCCCE
FFQHVTSPGVLYLNDMRWPLSDPILRQAISLAINRTAITYLVYYNYTTPAIDLPVSVGQL
EEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCEECCCCCCCC
GFFNSTVIQLAQELAPPQGNVTAALQLLESHGYKLINGQLIAPNGTPVPTMTIEAPAGST
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEECCCCCE
VWDAEVNLYAQQLKQIGLNVQVVIPPFSTALNDLLSGNFWMERMYLLSTGPTPIMFFQAF
EEECHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
LYNPVNTPGNITPIGNSTIYNVGRLNLSLRLSPNSPPIIKLYQYAMGNISNVAEYEKLLN
HCCCCCCCCCEEECCCCEEEEEEEEEEEEEECCCCCCEEEEHHHHCCCCCHHHHHHHHHH
EIGMFWLKYMPAIVLINGWVNYEYINSSISGWVTPQHNYWIGAPWYNPPLPVILSLHLAN
HHHHHHHHHCCEEEEEECCEEEEEECCCCCEEECCCCCEEEECCCCCCCHHHEEEEEECC
QSVPEPWWYYESQFNITVPSSWLTSNDPFVYSTSTTTTSTSTRTTSATTSTTTSTSTVIT
CCCCCCCEEEECEEEEECCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCCCCEEEE
TSVSTTTSVSTSVSTTTSTVTSTVTSSSSNTTLYVIIAVVVIIIVIIGVVLGLRRR
EECCCCCCCCCCCCCCHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA