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The map label for this gene is nikA [H]

Identifier: 227828563

GI number: 227828563

Start: 2124690

End: 2126852

Strand: Direct

Name: nikA [H]

Synonym: M1425_2314

Alternate gene names: 227828563

Gene position: 2124690-2126852 (Clockwise)

Preceding gene: 227828562

Following gene: 227828566

Centisome position: 81.44

GC content: 38.83

Gene sequence:

>2163_bases
ATGAAAAGGTACAAGATAATTTCTACAATAATCACATTATTGATGTTAATAAGCATAGGAATATTTGCAATGCCAATACT
ATCACAGCCAACATCAGTACAACCAGAAGGAAGTATGGTAGTTATGCCTTCTCCGGGCATAGTATGGCAAGATAATTTTA
ATCCATGGAACGCTCCAGCTTTACCCGGTGGTCTAATTTGGCTTATTTACGAACCACTCGCCCTAGTTAATTCGCTAAGT
GGTCAAACTATTCCATGGTTAGCCACCAATTGGACATTTACTACTGGTTATGTTTACTTGCCAAATGGTACTAAGGTACA
AACATTGGCATTAATATTATACTTAAGGCATGATGTATACTTCACTGATGGTACACCATTTAACGCTACAGCAGTATGGT
ACACATTAGCGTTAGAACAAGCGTACCCACAACTGGGATATTCGGCTAATCAAATAGCTAACATGACAATAATAAATCCA
TATGAGCTAGAAGTGGTATTTAAACCTGGAGTAACACCAATGGTATTGTATACTATTTTGGAGCAATGGCCTGTGGACCC
AGCACAATGGGGTAAAATATTCCCAGTAGAGCAATTACCAAATGGAACATATCTAGGACTAAACAAGACTGGAAATCCAT
TCACATACCCTAATACAAATCCAATAGGTACTGGACCTTATATGCTATATAGTTTCAGCCCGCAAGAAATAGTACTAGTG
GCCAACCCACACTATTGGATGCCGGGAGAACCCAGAATAAAATACTTACTCTATCCATCATATCCTAGTAATGTTCAAGC
AGATACTGCATTAAATAATGGGCAAATAACTTGGGCTGGACTATTCGAACCAAATATACAACAGCAATTTGTGGCAAAAG
ATCCTCTACACTATCATTACTATTTTGCTCCTGGATTTCCAACAATGTTGACACTCAATAACCTAAAATGGCCTTTATCC
GATCCAGTTTTAAGACAAGCAATAAGCCTTGCGATCAATAGAACAGCAATATACTATTTGGGCGAATATGGATACGAACC
GCCAGCAACTACGCCTCTTCCATTACCTCCACAACAGATACAATATCTAAATTCTTCGGTATTGCAGCTTGCTGAGCAGT
TTGCTCCATCTCAAGGTAATGTTACTGCTGCATTACAGTTACTTGAATCCCATGGCTATAAATTAGTTAATGGCCAACTA
ATTGCACCAAATGGTACTGCAGTACCAACAATGACCATAATTACGGTAGCCGGATGGACTGATTGGGATGCTGATTTAAC
TATAATTGCACAGGATTTGAAGCAAATAGGCATAACTGTGGAAGTACAAACACCACCGTTTACAACATGGTTTAATTACA
TGGAAACTGGCAATTACTGGATGGGACTAATGTGGGATTTAATTAGCGGTCCTACTCCTATACAACCACTTTCTGGGTAT
TTGTATGGTTATTGGAATTCCCCCGGCAACATAACACCGATAGGAAATAGCACATATTTTAACATAGAAAGGTTTAATTT
ATCAATTATCCATCCAACGTTCGAACAACTTGTGAACTGGGCAAATGGTAACTTCAGCATAAACGATAATGCCTATTATA
GTATAGTAAATAAGCTAGCTGCTCTATGGATAAAATATATGCCTTCCATTGCTCTAGTATACGGCGCAGAGTGGTATGAG
TATGTTAACAATACTGTTACTGGTTGGCCAACTCAGCAAAACTATTATTGGCCGGCTCCCCCATGGACCAGTCTTCCCTC
AACTCCTTTACCTGTTGTTTTAGCCTTACATTTGGTTAATCAGTCTGTTCCTGAGCCTTGGTGGTATTATACTTCGCAAG
TTCCTTCAAGTTGGTATACTTCCAATGACCCATTCGTGTATCAAACAACCACAACTACTACAACACCTAGTACTACCACA
AGTACATCAACAACTACTACAACAACCACATCCACCACAACAAGTACTACTACTACAACAACAAGTACTACTACTACGAG
TATTTCAACTACCACAAGTGTCTCTACATCCGTAAGCACTACTACGGCTACTGTTACTACTACTGTAAGCTCATCATCCA
ATACCGCACTATATGCCATAATAGCAGTTGTAATAATAGTCATAATAATAGCTGTTGTAGTACTAGGCCTAAGAAGAAGA
TAA

Upstream 100 bases:

>100_bases
GTATCACTAATTAGGTTGCAAGGAAATTGTGATTTTAGTAGTGAAGAAAAGTTTAAATATGGCTAAAATGCTATTATAAA
TCGATAAGTGGTAGATAGGT

Downstream 100 bases:

>100_bases
AAAAATAAATAAAGATTAAAAAGTTTTTTATATTTATTTTATTCCCATCTTATTATTACTTTTATTTCTCCTGGAAGTTT
TTCCCTAAGGCTACTTATAA

Product: extracellular solute-binding protein family 5

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MKRYKIISTIITLLMLISIGIFAMPILSQPTSVQPEGSMVVMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQTIPWLATNWTFTTGYVYLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEVVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYLGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQIQYLNSSVLQLAEQFAPSQGNVTAALQLLESHGYKLVNGQL
IAPNGTAVPTMTIITVAGWTDWDADLTIIAQDLKQIGITVEVQTPPFTTWFNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYFNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTPSTTT
STSTTTTTTTSTTTSTTTTTTSTTTTSISTTTSVSTSVSTTTATVTTTVSSSSNTALYAIIAVVIIVIIIAVVVLGLRRR

Sequences:

>Translated_720_residues
MKRYKIISTIITLLMLISIGIFAMPILSQPTSVQPEGSMVVMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQTIPWLATNWTFTTGYVYLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEVVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYLGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQIQYLNSSVLQLAEQFAPSQGNVTAALQLLESHGYKLVNGQL
IAPNGTAVPTMTIITVAGWTDWDADLTIIAQDLKQIGITVEVQTPPFTTWFNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYFNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTPSTTT
STSTTTTTTTSTTTSTTTTTTSTTTTSISTTTSVSTSVSTTTATVTTTVSSSSNTALYAIIAVVIIVIIIAVVVLGLRRR
>Mature_720_residues
MKRYKIISTIITLLMLISIGIFAMPILSQPTSVQPEGSMVVMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQTIPWLATNWTFTTGYVYLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEVVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYLGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQIQYLNSSVLQLAEQFAPSQGNVTAALQLLESHGYKLVNGQL
IAPNGTAVPTMTIITVAGWTDWDADLTIIAQDLKQIGITVEVQTPPFTTWFNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYFNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTPSTTT
STSTTTTTTTSTTTSTTTTTTSTTTTSISTTTSVSTSVSTTTATVTTTVSSSSNTALYAIIAVVIIVIIIAVVVLGLRRR

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=406, Percent_Identity=27.0935960591133, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI1787052, Length=340, Percent_Identity=25, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 80104; Mature: 80104

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYKIISTIITLLMLISIGIFAMPILSQPTSVQPEGSMVVMPSPGIVWQDNFNPWNAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCC
LPGGLIWLIYEPLALVNSLSGQTIPWLATNWTFTTGYVYLPNGTKVQTLALILYLRHDVY
CCCCEEEEEHHHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHHHHCEEE
FTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINPYELEVVFKPGVTPMVLYTIL
EECCCCCCCCEEEEEEHHHHHHHHCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHH
EQWPVDPAQWGKIFPVEQLPNGTYLGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
HHCCCCHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHY
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEE
YFAPGFPTMLTLNNLKWPLSDPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQI
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHH
QYLNSSVLQLAEQFAPSQGNVTAALQLLESHGYKLVNGQLIAPNGTAVPTMTIITVAGWT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEEEEECCC
DWDADLTIIAQDLKQIGITVEVQTPPFTTWFNYMETGNYWMGLMWDLISGPTPIQPLSGY
CCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
LYGYWNSPGNITPIGNSTYFNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLA
EEEEECCCCCEEECCCCEEEEEEEEEEEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ALWIKYMPSIALVYGAEWYEYVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVN
HHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
QSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTPSTTTSTSTTTTTTTSTTTSTTTTT
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEE
TSTTTTSISTTTSVSTSVSTTTATVTTTVSSSSNTALYAIIAVVIIVIIIAVVVLGLRRR
CCEEEEEEEEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRYKIISTIITLLMLISIGIFAMPILSQPTSVQPEGSMVVMPSPGIVWQDNFNPWNAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCC
LPGGLIWLIYEPLALVNSLSGQTIPWLATNWTFTTGYVYLPNGTKVQTLALILYLRHDVY
CCCCEEEEEHHHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHHHHCEEE
FTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINPYELEVVFKPGVTPMVLYTIL
EECCCCCCCCEEEEEEHHHHHHHHCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHH
EQWPVDPAQWGKIFPVEQLPNGTYLGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
HHCCCCHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHY
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEE
YFAPGFPTMLTLNNLKWPLSDPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQI
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHH
QYLNSSVLQLAEQFAPSQGNVTAALQLLESHGYKLVNGQLIAPNGTAVPTMTIITVAGWT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEEEEECCC
DWDADLTIIAQDLKQIGITVEVQTPPFTTWFNYMETGNYWMGLMWDLISGPTPIQPLSGY
CCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
LYGYWNSPGNITPIGNSTYFNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLA
EEEEECCCCCEEECCCCEEEEEEEEEEEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ALWIKYMPSIALVYGAEWYEYVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVN
HHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
QSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTPSTTTSTSTTTTTTTSTTTSTTTTT
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEE
TSTTTTSISTTTSVSTSVSTTTATVTTTVSSSSNTALYAIIAVVIIVIIIAVVVLGLRRR
CCEEEEEEEEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]