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The map label for this gene is pip [H]

Identifier: 227828498

GI number: 227828498

Start: 2058290

End: 2059201

Strand: Direct

Name: pip [H]

Synonym: M1425_2247

Alternate gene names: 227828498

Gene position: 2058290-2059201 (Clockwise)

Preceding gene: 227828497

Following gene: 227828499

Centisome position: 78.9

GC content: 35.42

Gene sequence:

>912_bases
ATGAAAGGTTATTCTATAAATGTGGAGGAAGGTTATAAGAGAATCTTTGGAATAAACATATACTATAAGTTGTATAGAGT
AAAGGGTAGTAATAGGAATTTGGTCACTTTGCATGGCGGCCCTGGAGGTTCTCATGATTACCTAATACCTTTAGCTGATT
TGTCAAATTACGGAATTAACGTCTTATTTTATGACCAATTTGGTTGCGGTAGGTCCGATGACCCTAAAAATACTTCTGAT
TATACCATAGATCACGGCTTAGAGGAATTAGAGGAACTTAGAAAGCAAGTTTTCGGCAATGATAAGATTGTAGTATTGGG
ACACTCCTACGGTGGAGCGTTGGCAATAGCTTATGCACTTAAGTATCAACAGTTTTTAAGAGGTCTTATTGTAAGTAGTG
GACTATCAAGTGTTCCCTATACCGTTAAAGAGATGAGGAGATTAATTGAGGAATTACCAGATAAGTACAAGACAATTATA
AAGAGATATGAAAGTTTAGGTGATTTCAAAAACCCAGAATATTTAGATGCCGTAAACTTCTTCTATAGTCAGCATCTATT
GAGACTTAAGGAAATGCCGGAACCAGTTAAAAGGACTTTCGAGTACATAGAAAAAAGGAGGACGTATGAAATAATGAATG
GTCCGAACGAGTTTACTATCATTGGAACAATAAAGGATTGGGACGTTACTGAGCAATTGTATAAAATAACTGTTCCTACT
CTAATAACCGTGGGAAAATACGATGAAGTAACTGTTAATATAGCTCAGCTTATTCATAAAAATATTAAGGGATCTAGACT
TGTCATTTTCGAGAATAGCTCCCATATGGCCATGTGGGAGGAAAAGGATAAGTATCTTGAAGTTATAAAGGAGTTTATCG
ATCAAGTGTATTCACTTAATATGGTTAAGTGA

Upstream 100 bases:

>100_bases
AGTATACGAAGGCTGGTAGTTAATTCTTTTTTCTCATATCATCTTAATTCAAAGTTGCAAGTCAAGGTTTTTTATCATCT
TGTCATACTATTTTATAACT

Downstream 100 bases:

>100_bases
TTCCTTTTTATAATACTAATGAAGTTTTCGTTAAAATCCAATAGCGAGTTAATGTGATTGATACTATCACAATAATAATA
TAAGTATATTTAGGTCAGCA

Product: proline-specific peptidase

Products: NA

Alternate protein names: PIP; Prolyl aminopeptidase; PAP; Tricorn protease-interacting factor F1 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGINVLFYDQFGCGRSDDPKNTSD
YTIDHGLEELEELRKQVFGNDKIVVLGHSYGGALAIAYALKYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTII
KRYESLGDFKNPEYLDAVNFFYSQHLLRLKEMPEPVKRTFEYIEKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPT
LITVGKYDEVTVNIAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLNMVK

Sequences:

>Translated_303_residues
MKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGINVLFYDQFGCGRSDDPKNTSD
YTIDHGLEELEELRKQVFGNDKIVVLGHSYGGALAIAYALKYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTII
KRYESLGDFKNPEYLDAVNFFYSQHLLRLKEMPEPVKRTFEYIEKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPT
LITVGKYDEVTVNIAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLNMVK
>Mature_303_residues
MKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGINVLFYDQFGCGRSDDPKNTSD
YTIDHGLEELEELRKQVFGNDKIVVLGHSYGGALAIAYALKYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTII
KRYESLGDFKNPEYLDAVNFFYSQHLLRLKEMPEPVKRTFEYIEKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPT
LITVGKYDEVTVNIAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLNMVK

Specific function: Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. In conjunction with the three factors F1, F2 and F3, Tricorn degrades oligopeptides in a sequential manner, yielding

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S33 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR005945
- InterPro:   IPR002410 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.4.11.5 [H]

Molecular weight: Translated: 35127; Mature: 35127

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGIN
CCCCCCCHHHHHHHHHEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCEE
VLFYDQFGCGRSDDPKNTSDYTIDHGLEELEELRKQVFGNDKIVVLGHSYGGALAIAYAL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTIIKRYESLGDFKNPEYLDAVNF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
FYSQHLLRLKEMPEPVKRTFEYIEKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPT
HHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECCCCHHHHHHHEEHHH
LITVGKYDEVTVNIAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLN
EEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCC
MVK
CCC
>Mature Secondary Structure
MKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGIN
CCCCCCCHHHHHHHHHEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCEE
VLFYDQFGCGRSDDPKNTSDYTIDHGLEELEELRKQVFGNDKIVVLGHSYGGALAIAYAL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTIIKRYESLGDFKNPEYLDAVNF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
FYSQHLLRLKEMPEPVKRTFEYIEKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPT
HHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECCCCHHHHHHHEEHHH
LITVGKYDEVTVNIAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLN
EEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHCC
MVK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]