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The map label for this gene is ilvG [H]

Identifier: 227828407

GI number: 227828407

Start: 1968968

End: 1970581

Strand: Direct

Name: ilvG [H]

Synonym: M1425_2150

Alternate gene names: 227828407

Gene position: 1968968-1970581 (Clockwise)

Preceding gene: 227828405

Following gene: 227828408

Centisome position: 75.47

GC content: 38.41

Gene sequence:

>1614_bases
ATGAATGCGGGAAAGATATTCTTATCTCTGCTTAAAGAAAGTGGAATTAATAAGATATTTATAGTATCTGGCACGGATTA
CGCTTCATTAATAGAGGCTAAAGTTGAAGATCCTAGCTTGCCAGATTTTGAAATAGTACCCCACGAGATTACCGCAATTT
CGACCGCAATAGGTTATGCACTGGGCAATAAGCTAAGTGCCGTTGCAGTTCACACTACACCCGGGACTGCAAACGCGTTA
GGAGGTATAATGAGTGCGTATACTTCTAGAATACCACTTTTAGTTATTGCGGGAAGGAGTCCATATACTGAGAAAGGTAG
TACTGCAAGCAGGAACTTGAGAATTCATTGGACGCAAGAGGCTAGGGATCAAGGAGAATTGGTAAGGCAATATGTTAAGT
ATGATTTTGAAATTAGGATGGCTGATCAGATACCAGCAGCAATATCTAGAGCAATCCAGATAATGATGAGTGAACCTAGA
GGTCCAGTATATCTTGTATTGCCAAGAGAGGTAAGCGTTCAAGAAGTTAATGAAGTTAAGAGAATACCAATGGACTATTA
TGAACCCGCACCTTCGCCAGAGAAAATCAACAAAGCTAAGGAGATGCTGGAAAAGTCTGAAAGACCGGTAATTATCACGT
GGAGGGCTGGAAGGAGAAAAGAATGGTTTGAGTCCCTAAGGAGATTTGCAGATAGTTACAATATTCCAGTTCTCAATTAT
GCTGGGGAGGTTTTGAATTATCCCAGTAATGGGGCAATGGCCTTGGATAGATTTGATTTACGAAATGCTGATTTATTATT
AGTAGTGGAGGCTGAAGTGCCATATTTTCCTAAGAAAATTGATTTAGATATCCCAATAATTAAGGTTGATGTAGAACCTT
CATATTCTTATATTCCATACTACGGTTTTAGATGTGATCTATGTATACAATCCATTCCCAGCAACTTCTTTGATTATATC
TCAATAAGAGCAAAGAGCTATGATGAAATTAGAGAGCTAAAGATAAGGCAGGAAGAATATAAGAAGCAAGAGATTGAGAG
GTTAAAAGATAGGAAATCAATTCATCCTAAATACTTATCGTATGAAATCGGGAATGTTGCTTCTGAGTATAATTTAGCGA
TATTTAATGAATATCAGTTTAATCCTAGATATGCTAAGCTAAACGAATTTGGTTCTTATTTTGCTGACTTATCCATAGGT
TATCTAGGCCTTGCATTAGGAGCTGGAGTTGGGTATAAAATGGCTACTAATAAGGACGTACTTATCACTACAGGTGATGG
TTCCTTCATATTTGGCGTTCCAGAAGCCTATTATTACGTGTCCTCAAAGTATCCTACAATGGTTGTGATTTTTGACAACG
GTGGTTGGTTAGCGTCAGCTGAGGCAGTAGATGAGGTTTTCCCTGAGGGTTTGGCAAAAAGTAAAAGGTACTATCCCGGA
GCAGATTTTGATAAGAGATTCGAGATCGGAAAGACTGTTGAAGCTTTTCATGGCTACTATGAACTAGTTGAGGATCCTTG
GGAGATTAAGTCTGCCTTAATAAGGGGTTTAGAGAAAGTGAGAAAAGAGAATAAAATAGCTGTGATTCAAGTTATAGTGG
ATAAGGTGAGGTAA

Upstream 100 bases:

>100_bases
AGGCAGAGTATTCATTTATCGTAAACAATAAATTAACACCATTTAATATTTTCTTTGGAGTGAAAGATAGTATTTTATAT
TCATGATAAAATATTAAGAT

Downstream 100 bases:

>100_bases
TTATGAAGTTATGTAACAAAGAGATATCGCAAATTGGGTTTGGGACCTGGAAAATTGGCGGAGGTTACTGGAATCCAGAT
TATTCTATGGATTCCTATTA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNAGKIFLSLLKESGINKIFIVSGTDYASLIEAKVEDPSLPDFEIVPHEITAISTAIGYALGNKLSAVAVHTTPGTANAL
GGIMSAYTSRIPLLVIAGRSPYTEKGSTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQIPAAISRAIQIMMSEPR
GPVYLVLPREVSVQEVNEVKRIPMDYYEPAPSPEKINKAKEMLEKSERPVIITWRAGRRKEWFESLRRFADSYNIPVLNY
AGEVLNYPSNGAMALDRFDLRNADLLLVVEAEVPYFPKKIDLDIPIIKVDVEPSYSYIPYYGFRCDLCIQSIPSNFFDYI
SIRAKSYDEIRELKIRQEEYKKQEIERLKDRKSIHPKYLSYEIGNVASEYNLAIFNEYQFNPRYAKLNEFGSYFADLSIG
YLGLALGAGVGYKMATNKDVLITTGDGSFIFGVPEAYYYVSSKYPTMVVIFDNGGWLASAEAVDEVFPEGLAKSKRYYPG
ADFDKRFEIGKTVEAFHGYYELVEDPWEIKSALIRGLEKVRKENKIAVIQVIVDKVR

Sequences:

>Translated_537_residues
MNAGKIFLSLLKESGINKIFIVSGTDYASLIEAKVEDPSLPDFEIVPHEITAISTAIGYALGNKLSAVAVHTTPGTANAL
GGIMSAYTSRIPLLVIAGRSPYTEKGSTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQIPAAISRAIQIMMSEPR
GPVYLVLPREVSVQEVNEVKRIPMDYYEPAPSPEKINKAKEMLEKSERPVIITWRAGRRKEWFESLRRFADSYNIPVLNY
AGEVLNYPSNGAMALDRFDLRNADLLLVVEAEVPYFPKKIDLDIPIIKVDVEPSYSYIPYYGFRCDLCIQSIPSNFFDYI
SIRAKSYDEIRELKIRQEEYKKQEIERLKDRKSIHPKYLSYEIGNVASEYNLAIFNEYQFNPRYAKLNEFGSYFADLSIG
YLGLALGAGVGYKMATNKDVLITTGDGSFIFGVPEAYYYVSSKYPTMVVIFDNGGWLASAEAVDEVFPEGLAKSKRYYPG
ADFDKRFEIGKTVEAFHGYYELVEDPWEIKSALIRGLEKVRKENKIAVIQVIVDKVR
>Mature_537_residues
MNAGKIFLSLLKESGINKIFIVSGTDYASLIEAKVEDPSLPDFEIVPHEITAISTAIGYALGNKLSAVAVHTTPGTANAL
GGIMSAYTSRIPLLVIAGRSPYTEKGSTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQIPAAISRAIQIMMSEPR
GPVYLVLPREVSVQEVNEVKRIPMDYYEPAPSPEKINKAKEMLEKSERPVIITWRAGRRKEWFESLRRFADSYNIPVLNY
AGEVLNYPSNGAMALDRFDLRNADLLLVVEAEVPYFPKKIDLDIPIIKVDVEPSYSYIPYYGFRCDLCIQSIPSNFFDYI
SIRAKSYDEIRELKIRQEEYKKQEIERLKDRKSIHPKYLSYEIGNVASEYNLAIFNEYQFNPRYAKLNEFGSYFADLSIG
YLGLALGAGVGYKMATNKDVLITTGDGSFIFGVPEAYYYVSSKYPTMVVIFDNGGWLASAEAVDEVFPEGLAKSKRYYPG
ADFDKRFEIGKTVEAFHGYYELVEDPWEIKSALIRGLEKVRKENKIAVIQVIVDKVR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=21.6783216783217, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788716, Length=502, Percent_Identity=23.7051792828685, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787096, Length=423, Percent_Identity=23.8770685579196, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786717, Length=190, Percent_Identity=28.4210526315789, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531301, Length=508, Percent_Identity=23.4251968503937, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531299, Length=508, Percent_Identity=23.4251968503937, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=518, Percent_Identity=20.8494208494208, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922626, Length=445, Percent_Identity=23.1460674157303, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60933; Mature: 60933

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGKIFLSLLKESGINKIFIVSGTDYASLIEAKVEDPSLPDFEIVPHEITAISTAIGYA
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHH
LGNKLSAVAVHTTPGTANALGGIMSAYTSRIPLLVIAGRSPYTEKGSTASRNLRIHWTQE
HCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECHH
ARDQGELVRQYVKYDFEIRMADQIPAAISRAIQIMMSEPRGPVYLVLPREVSVQEVNEVK
HHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
RIPMDYYEPAPSPEKINKAKEMLEKSERPVIITWRAGRRKEWFESLRRFADSYNIPVLNY
CCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEC
AGEVLNYPSNGAMALDRFDLRNADLLLVVEAEVPYFPKKIDLDIPIIKVDVEPSYSYIPY
CHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCEECCCCEEEEEECCCCCCCCCC
YGFRCDLCIQSIPSNFFDYISIRAKSYDEIRELKIRQEEYKKQEIERLKDRKSIHPKYLS
CCCCHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YEIGNVASEYNLAIFNEYQFNPRYAKLNEFGSYFADLSIGYLGLALGAGVGYKMATNKDV
EECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
LITTGDGSFIFGVPEAYYYVSSKYPTMVVIFDNGGWLASAEAVDEVFPEGLAKSKRYYPG
EEEECCCCEEEECCCEEEEEECCCCEEEEEECCCCEEECHHHHHHHHHHHHHHCCCCCCC
ADFDKRFEIGKTVEAFHGYYELVEDPWEIKSALIRGLEKVRKENKIAVIQVIVDKVR
CCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCC
>Mature Secondary Structure
MNAGKIFLSLLKESGINKIFIVSGTDYASLIEAKVEDPSLPDFEIVPHEITAISTAIGYA
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHH
LGNKLSAVAVHTTPGTANALGGIMSAYTSRIPLLVIAGRSPYTEKGSTASRNLRIHWTQE
HCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECHH
ARDQGELVRQYVKYDFEIRMADQIPAAISRAIQIMMSEPRGPVYLVLPREVSVQEVNEVK
HHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
RIPMDYYEPAPSPEKINKAKEMLEKSERPVIITWRAGRRKEWFESLRRFADSYNIPVLNY
CCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEC
AGEVLNYPSNGAMALDRFDLRNADLLLVVEAEVPYFPKKIDLDIPIIKVDVEPSYSYIPY
CHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCEECCCCEEEEEECCCCCCCCCC
YGFRCDLCIQSIPSNFFDYISIRAKSYDEIRELKIRQEEYKKQEIERLKDRKSIHPKYLS
CCCCHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YEIGNVASEYNLAIFNEYQFNPRYAKLNEFGSYFADLSIGYLGLALGAGVGYKMATNKDV
EECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
LITTGDGSFIFGVPEAYYYVSSKYPTMVVIFDNGGWLASAEAVDEVFPEGLAKSKRYYPG
EEEECCCCEEEECCCEEEEEECCCCEEEEEECCCCEEECHHHHHHHHHHHHHHCCCCCCC
ADFDKRFEIGKTVEAFHGYYELVEDPWEIKSALIRGLEKVRKENKIAVIQVIVDKVR
CCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]