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The map label for this gene is 227828296

Identifier: 227828296

GI number: 227828296

Start: 1869381

End: 1870433

Strand: Direct

Name: 227828296

Synonym: M1425_2037

Alternate gene names: NA

Gene position: 1869381-1870433 (Clockwise)

Preceding gene: 227828295

Following gene: 227828298

Centisome position: 71.66

GC content: 34.09

Gene sequence:

>1053_bases
TTGACCATTATAGGAAATCCAGAGATAGGACTATACACTGGAAAATTACCTAAAGGATGTGAACTCTGTAGGTTAGGAGG
TAAATTAGTCCTCTTTATCACTGGCGAATGCGATGACAACTGCTATTATTGTCCCGTAAGTGATGACAGATTTGGAAAAA
ATAGGATATTTGCAAATGAGACTGAAGTAAAAGACGCTATGGACGTCATATATGAGGCATATAGGATGAAAGCTCTAGGT
GCGGGAATAACTGGTGGTGATCCTATTCTTGAAATAGGTAGAGTAATTGAGATTATTGACAAACTAAAGAATGAATTCGG
TTCGGATTTTCATATTCATCTTTACACTACTGGACGTTATGTTAATGACGATGTAATGAAGGAGCTGGTTAGTGCAGGGC
TGGATGAGATACGTTTTCACCCAGTAAAAGAGCTATATCTAAATGCAGTAAAGATAGCTTTAAAGTACGATATTGATGTA
GGTTTAGAATTACCAGCTATTCCGAACGAGGGGGATAAAATTGTCAGATTAGTTAAATGGGCTGAGGAAAATAATGTTAA
GTTTGTAAATTTAAATGAGCTCGAGCTAAATGCTAGGAACTATTTGAATCTAAATAGTAAAGGTCTTCATGCTAAACATG
GAATAACGGGAGTAGAAGGTAGTTTTAATACTGCCTTAGATGTTCTAAAAAAATTTGAAGAAGACAAAAAGATTACTGTC
CATTATTGTAGTTCTGTTTATAAAGATCTGGTGGAAACTAGAACTAGATTTTTTAGAATAATCAAATATAATTCAAAACC
CTATGAGGAGTATACTAATGAGGCCACAATTGTAAGAGCAATAGTTAAGACCAACCATAAGATTCCCTTATTGGAGAATT
TTGGTGAGTTTAATGGTTCAAATGAATACTCTGTTTCGCCAAGTATTATTAAAGATCTATTAAATGAGTACACTGATTTA
ATAGACGAAATTTATATAGTTGAGGAGCACCCTGATTCACGAAGATTAAGAGTAGGAGAGAAGCTAATTTATTCTAAATC
TAAGAATAGCTAA

Upstream 100 bases:

>100_bases
TCAAATAAGCCCCTTAATGGTTATGAGGGAGTCAGACAGCTAGAGGTAATAAGAGAAGAAATTAAAAACGTGAATATCAT
TAAAAGGATAGATGAGTTAA

Downstream 100 bases:

>100_bases
TATACCAGTAAGATTCTTTAATTCAAAATAGATTGGCGATTCTTTAGGCACTAATATTATCTCCCCCCTTTTATTTTCTA
TATCCTCAAGAATTCTTTCA

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNRIFANETEVKDAMDVIYEAYRMKALG
AGITGGDPILEIGRVIEIIDKLKNEFGSDFHIHLYTTGRYVNDDVMKELVSAGLDEIRFHPVKELYLNAVKIALKYDIDV
GLELPAIPNEGDKIVRLVKWAEENNVKFVNLNELELNARNYLNLNSKGLHAKHGITGVEGSFNTALDVLKKFEEDKKITV
HYCSSVYKDLVETRTRFFRIIKYNSKPYEEYTNEATIVRAIVKTNHKIPLLENFGEFNGSNEYSVSPSIIKDLLNEYTDL
IDEIYIVEEHPDSRRLRVGEKLIYSKSKNS

Sequences:

>Translated_350_residues
MTIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNRIFANETEVKDAMDVIYEAYRMKALG
AGITGGDPILEIGRVIEIIDKLKNEFGSDFHIHLYTTGRYVNDDVMKELVSAGLDEIRFHPVKELYLNAVKIALKYDIDV
GLELPAIPNEGDKIVRLVKWAEENNVKFVNLNELELNARNYLNLNSKGLHAKHGITGVEGSFNTALDVLKKFEEDKKITV
HYCSSVYKDLVETRTRFFRIIKYNSKPYEEYTNEATIVRAIVKTNHKIPLLENFGEFNGSNEYSVSPSIIKDLLNEYTDL
IDEIYIVEEHPDSRRLRVGEKLIYSKSKNS
>Mature_349_residues
TIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNRIFANETEVKDAMDVIYEAYRMKALGA
GITGGDPILEIGRVIEIIDKLKNEFGSDFHIHLYTTGRYVNDDVMKELVSAGLDEIRFHPVKELYLNAVKIALKYDIDVG
LELPAIPNEGDKIVRLVKWAEENNVKFVNLNELELNARNYLNLNSKGLHAKHGITGVEGSFNTALDVLKKFEEDKKITVH
YCSSVYKDLVETRTRFFRIIKYNSKPYEEYTNEATIVRAIVKTNHKIPLLENFGEFNGSNEYSVSPSIIKDLLNEYTDLI
DEIYIVEEHPDSRRLRVGEKLIYSKSKNS

Specific function: Unknown

COG id: COG2108

COG function: function code R; Uncharacterized conserved protein related to pyruvate formate-lyase activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39854; Mature: 39723

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNRIFANE
CEEECCCCCEEEECCCCCCCHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCCCCEECCH
TEVKDAMDVIYEAYRMKALGAGITGGDPILEIGRVIEIIDKLKNEFGSDFHIHLYTTGRY
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
VNDDVMKELVSAGLDEIRFHPVKELYLNAVKIALKYDIDVGLELPAIPNEGDKIVRLVKW
CCHHHHHHHHHCCHHHHCCCCHHHHHHHHHEEEEEECCCCCEECCCCCCCCHHHHHHHHH
AEENNVKFVNLNELELNARNYLNLNSKGLHAKHGITGVEGSFNTALDVLKKFEEDKKITV
HCCCCEEEEEECCEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEH
HYCSSVYKDLVETRTRFFRIIKYNSKPYEEYTNEATIVRAIVKTNHKIPLLENFGEFNGS
HHHHHHHHHHHHHHHHHEEEEECCCCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHCCCCC
NEYSVSPSIIKDLLNEYTDLIDEIYIVEEHPDSRRLRVGEKLIYSKSKNS
CCCEECHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNRIFANE
EEECCCCCEEEECCCCCCCHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCCCCEECCH
TEVKDAMDVIYEAYRMKALGAGITGGDPILEIGRVIEIIDKLKNEFGSDFHIHLYTTGRY
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
VNDDVMKELVSAGLDEIRFHPVKELYLNAVKIALKYDIDVGLELPAIPNEGDKIVRLVKW
CCHHHHHHHHHCCHHHHCCCCHHHHHHHHHEEEEEECCCCCEECCCCCCCCHHHHHHHHH
AEENNVKFVNLNELELNARNYLNLNSKGLHAKHGITGVEGSFNTALDVLKKFEEDKKITV
HCCCCEEEEEECCEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEH
HYCSSVYKDLVETRTRFFRIIKYNSKPYEEYTNEATIVRAIVKTNHKIPLLENFGEFNGS
HHHHHHHHHHHHHHHHHEEEEECCCCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHCCCCC
NEYSVSPSIIKDLLNEYTDLIDEIYIVEEHPDSRRLRVGEKLIYSKSKNS
CCCEECHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]