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The map label for this gene is map [H]

Identifier: 227828285

GI number: 227828285

Start: 1858521

End: 1859426

Strand: Direct

Name: map [H]

Synonym: M1425_2026

Alternate gene names: 227828285

Gene position: 1858521-1859426 (Clockwise)

Preceding gene: 227828280

Following gene: 227828286

Centisome position: 71.24

GC content: 36.64

Gene sequence:

>906_bases
ATGACTGAGGATGAACTTAACAAGCTCTTATTAGCAGGAAAAATTGCATCTAAGGCTAGAGATGAAGTTTCCTTAAACGT
TAAGGCTAATGCCAAGGTTTTAGATATTTGTGAAGAGGTTGAGAGTATAATACTCGAAAATAAGGCATTTCCATCATTTC
CATGTAATATATCCATTAATTCAGAAGCAGCTCATTATAGTCCAGTTATAAATGATGAAAAGAGAATTCCGGAAGGTGCA
GTAGTTAAGTTAGATTTGGGAGCTCATATTGATGGATATATTAGTGATACCGCTACTACCATTAGTTTAGACGCTAAGTA
TCAAAGGTTGTTAGATGCCTCTAAAACCGCTCTTGAAGCTGCAATTGCTAATTTTAGGGCTGGACTAAGCGTAGGCGAGA
TAGGTAGAGTTATTGAAAAAATGATAAGAGCTCAAGGATATAAGCCGATAAGGAACTTGGGAGGTCATCTTATAAGGCGC
TATGAACTTCATGCTGGCGTCTTTATACCCAATGTTTATGAAAGGGGATTAGGCGCTATACAATCTGATTCTGTTTACGC
GATAGAGCCGTTCGCTACTGATGGGGGTGGTGAGGTAGTAGAGGGGAAGGATGTTACAATATATTCCTTAAAAAATCCCA
ATGTGAAAGGTCTTTCTGCGAGAGAGAGTGAACTGCTAGATTTTATATATACACATTTTAATTATCTGCCATTTTCAGAG
AGATGGCTTAAGGAATTTTCTACCAATGTCGACGAATTAAGAAATAATATAAAAAATTTAATTAAAAAAGGTGCATTAAG
AGGATATCCTATCCTACTTGAGATTAAAAAAGGCGTTGTTTCCCAGTTCGAGCATACCGTTATAGTTAAGGGCAATTCAA
TTATAGTGTCTACAAAGTCTCTTTGA

Upstream 100 bases:

>100_bases
AATGTTAACAAGGTTGCTGCATGGCCTAACCATCTTAACTGTGCCATACAAAAGATTTTTATGAGAAAAAAATAAAGCTT
ATACCCCGAAAACTACATTC

Downstream 100 bases:

>100_bases
AAAAAGTTTAATTAATTCGAATGAGTAAAGGTTAACGATAAAAATGAGTTTAATTGCTTTAGCACAAACATCTTTAAATC
AAGCAAGTTCTCTTTACTAC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTEDELNKLLLAGKIASKARDEVSLNVKANAKVLDICEEVESIILENKAFPSFPCNISINSEAAHYSPVINDEKRIPEGA
VVKLDLGAHIDGYISDTATTISLDAKYQRLLDASKTALEAAIANFRAGLSVGEIGRVIEKMIRAQGYKPIRNLGGHLIRR
YELHAGVFIPNVYERGLGAIQSDSVYAIEPFATDGGGEVVEGKDVTIYSLKNPNVKGLSARESELLDFIYTHFNYLPFSE
RWLKEFSTNVDELRNNIKNLIKKGALRGYPILLEIKKGVVSQFEHTVIVKGNSIIVSTKSL

Sequences:

>Translated_301_residues
MTEDELNKLLLAGKIASKARDEVSLNVKANAKVLDICEEVESIILENKAFPSFPCNISINSEAAHYSPVINDEKRIPEGA
VVKLDLGAHIDGYISDTATTISLDAKYQRLLDASKTALEAAIANFRAGLSVGEIGRVIEKMIRAQGYKPIRNLGGHLIRR
YELHAGVFIPNVYERGLGAIQSDSVYAIEPFATDGGGEVVEGKDVTIYSLKNPNVKGLSARESELLDFIYTHFNYLPFSE
RWLKEFSTNVDELRNNIKNLIKKGALRGYPILLEIKKGVVSQFEHTVIVKGNSIIVSTKSL
>Mature_300_residues
TEDELNKLLLAGKIASKARDEVSLNVKANAKVLDICEEVESIILENKAFPSFPCNISINSEAAHYSPVINDEKRIPEGAV
VKLDLGAHIDGYISDTATTISLDAKYQRLLDASKTALEAAIANFRAGLSVGEIGRVIEKMIRAQGYKPIRNLGGHLIRRY
ELHAGVFIPNVYERGLGAIQSDSVYAIEPFATDGGGEVVEGKDVTIYSLKNPNVKGLSARESELLDFIYTHFNYLPFSER
WLKEFSTNVDELRNNIKNLIKKGALRGYPILLEIKKGVVSQFEHTVIVKGNSIIVSTKSL

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI5803092, Length=307, Percent_Identity=32.5732899022801, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI124494254, Length=284, Percent_Identity=23.943661971831, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI164420681, Length=210, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17544396, Length=292, Percent_Identity=29.1095890410959, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17542840, Length=319, Percent_Identity=29.4670846394984, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319380, Length=320, Percent_Identity=27.1875, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137568, Length=305, Percent_Identity=31.4754098360656, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24658585, Length=317, Percent_Identity=25.5520504731861, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583427, Length=211, Percent_Identity=26.5402843601896, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355531, Length=207, Percent_Identity=23.6714975845411, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002468
- InterPro:   IPR018349
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 33191; Mature: 33060

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01202 MAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDELNKLLLAGKIASKARDEVSLNVKANAKVLDICEEVESIILENKAFPSFPCNISIN
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
SEAAHYSPVINDEKRIPEGAVVKLDLGAHIDGYISDTATTISLDAKYQRLLDASKTALEA
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCCEECCCEEEEEECHHHHHHHHHHHHHHHH
AIANFRAGLSVGEIGRVIEKMIRAQGYKPIRNLGGHLIRRYELHAGVFIPNVYERGLGAI
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEECCHHHHHCCCCC
QSDSVYAIEPFATDGGGEVVEGKDVTIYSLKNPNVKGLSARESELLDFIYTHFNYLPFSE
CCCCEEEEECEECCCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
RWLKEFSTNVDELRNNIKNLIKKGALRGYPILLEIKKGVVSQFEHTVIVKGNSIIVSTKS
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCEEEEECCEEEEEECC
L
C
>Mature Secondary Structure 
TEDELNKLLLAGKIASKARDEVSLNVKANAKVLDICEEVESIILENKAFPSFPCNISIN
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
SEAAHYSPVINDEKRIPEGAVVKLDLGAHIDGYISDTATTISLDAKYQRLLDASKTALEA
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCCEECCCEEEEEECHHHHHHHHHHHHHHHH
AIANFRAGLSVGEIGRVIEKMIRAQGYKPIRNLGGHLIRRYELHAGVFIPNVYERGLGAI
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEECCHHHHHCCCCC
QSDSVYAIEPFATDGGGEVVEGKDVTIYSLKNPNVKGLSARESELLDFIYTHFNYLPFSE
CCCCEEEEECEECCCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
RWLKEFSTNVDELRNNIKNLIKKGALRGYPILLEIKKGVVSQFEHTVIVKGNSIIVSTKS
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCEEEEECCEEEEEECC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]