Click here to switch to the map view.

The map label for this gene is gspE [C]

Identifier: 227828264

GI number: 227828264

Start: 1838926

End: 1840347

Strand: Direct

Name: gspE [C]

Synonym: M1425_2005

Alternate gene names: 227828264

Gene position: 1838926-1840347 (Clockwise)

Preceding gene: 227828263

Following gene: 227828265

Centisome position: 70.49

GC content: 33.19

Gene sequence:

>1422_bases
ATGAATATTCTTCAAGAGTATTCTGTTCTAAATGCTAAAGTCATTATATATGAGGATAATGGAATTGGCTATTATAAAGT
AAGCGAACCTAGCCTAGATCAGAAGGAAATTGAACTACTTAATTCTATATTAGAGTATATTTATTCAACTCCTAGTATAT
CTAATATAGAAGAGACAGTAGTCAAGATACTTAAGAATAAAGGTATTACTGATCAAGGTATTATTGAAAAAATGATCTAT
TATTTGAGAAAAAGGTTAGGTTATGATGAGCTAACTGTTCCCTTAGCAGATCCTTATGTTGAAGAAGTAGAATGCAAAGG
ATTCTCATACCCTATAACTGTAGTTCATAGAATTGTTACGAGATTCCCAAGATTGTATACCAATATAGTCTTTGAGCAAG
AGGATCATGTTCTAAAGGTTATTGAAAAACTTGCAAACAAGGCGGATAAGCCAGTCAGTATTGCCAAGCCATACCTTGAA
TTTTCCCTACCTGAGGGCCATAGAGTTGCTGCTACGGTATCTCGTGAGGTTTCATTACCTGGTTCTACATTTGATATAAG
GAAATTTCCTTTAAAGCCAATAAGTCCTATATTATTGATTAAAAACGTATCGATAAGTTCGCTAATGTTAGCTTATATTT
GGTTTTTATTGGATTATAAACCGTTCTTTTTAGTCATAGGTTCTACCGGATCTGGAAAGACTACCTTACTTAACTCGATA
CTCAGTATGGTTAACCCTTTCTTTAAGGTAATTACGATTGAGGATACTCCAGAAATAAACATAGTTCATGATAACTGGAT
AAGGTTTTTTGCTAGACAATCAATCTCTTCCCAATTTGAAGTATCGTTGATGGATTTAGCTAGACTATCATTAAGATATA
GGCCGGATTACTTAGTTATAGGTGAGATTCGAGGCAAGGAGATAGAGGCATTGGTTCATGCCTCAGCTTCTGGCCATGGT
TCGTTAGCCACATTTCATGCAGGCAATCCACATGAGGCCCTAACCAGATTAATTAGTCTTTTAAATAAGGATGTTGCTAA
ATTGTTTTTACAAAATCTATGGGGGATTATAGTTTTAGGAACATTAAGAGATCGTAATGGGAATATAAGGCGCATTATAA
GGTCGATTTATGAAATAGTTTACGTTAAAGGTAAACTAAGATTCAAACCAGTGTTAAAATGGTCTTTCTCCTCTAACACA
TTTTTACCTAACAGTGTTAATGATTTAGTTAAAAAGTCCTATAGGATCAATTATATATCAAAGACATATGAAATACCAAT
TTCAGAAATAACAGAGGAGATTAGTAATAGAGTTAAAATCCTTGATAAGTTAGTAAATAATGAGGTCTTAGATCTTGAAG
ATTTTAGTATTTATCTAAAGAAAATAACCCAAGGTGGTCAAGTTGAGACAAAGGCTATTTGA

Upstream 100 bases:

>100_bases
GTTATCAATCTCTATGGCTTTAAGTGAAGCAATGGCTATAAAGGAAGTTTTAAGTAATTTCGGTATTGTGTGAGTATATT
TTTTCTGAATACTCAATATT

Downstream 100 bases:

>100_bases
AAAAATTAATTTATTTAACTTGATTGCTACGAGAATAAATGATAATATTAAATATTATGGTGATGACATAGAAAGACTCC
GTAAAGAGTATACTAGGATA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNILQEYSVLNAKVIIYEDNGIGYYKVSEPSLDQKEIELLNSILEYIYSTPSISNIEETVVKILKNKGITDQGIIEKMIY
YLRKRLGYDELTVPLADPYVEEVECKGFSYPITVVHRIVTRFPRLYTNIVFEQEDHVLKVIEKLANKADKPVSIAKPYLE
FSLPEGHRVAATVSREVSLPGSTFDIRKFPLKPISPILLIKNVSISSLMLAYIWFLLDYKPFFLVIGSTGSGKTTLLNSI
LSMVNPFFKVITIEDTPEINIVHDNWIRFFARQSISSQFEVSLMDLARLSLRYRPDYLVIGEIRGKEIEALVHASASGHG
SLATFHAGNPHEALTRLISLLNKDVAKLFLQNLWGIIVLGTLRDRNGNIRRIIRSIYEIVYVKGKLRFKPVLKWSFSSNT
FLPNSVNDLVKKSYRINYISKTYEIPISEITEEISNRVKILDKLVNNEVLDLEDFSIYLKKITQGGQVETKAI

Sequences:

>Translated_473_residues
MNILQEYSVLNAKVIIYEDNGIGYYKVSEPSLDQKEIELLNSILEYIYSTPSISNIEETVVKILKNKGITDQGIIEKMIY
YLRKRLGYDELTVPLADPYVEEVECKGFSYPITVVHRIVTRFPRLYTNIVFEQEDHVLKVIEKLANKADKPVSIAKPYLE
FSLPEGHRVAATVSREVSLPGSTFDIRKFPLKPISPILLIKNVSISSLMLAYIWFLLDYKPFFLVIGSTGSGKTTLLNSI
LSMVNPFFKVITIEDTPEINIVHDNWIRFFARQSISSQFEVSLMDLARLSLRYRPDYLVIGEIRGKEIEALVHASASGHG
SLATFHAGNPHEALTRLISLLNKDVAKLFLQNLWGIIVLGTLRDRNGNIRRIIRSIYEIVYVKGKLRFKPVLKWSFSSNT
FLPNSVNDLVKKSYRINYISKTYEIPISEITEEISNRVKILDKLVNNEVLDLEDFSIYLKKITQGGQVETKAI
>Mature_473_residues
MNILQEYSVLNAKVIIYEDNGIGYYKVSEPSLDQKEIELLNSILEYIYSTPSISNIEETVVKILKNKGITDQGIIEKMIY
YLRKRLGYDELTVPLADPYVEEVECKGFSYPITVVHRIVTRFPRLYTNIVFEQEDHVLKVIEKLANKADKPVSIAKPYLE
FSLPEGHRVAATVSREVSLPGSTFDIRKFPLKPISPILLIKNVSISSLMLAYIWFLLDYKPFFLVIGSTGSGKTTLLNSI
LSMVNPFFKVITIEDTPEINIVHDNWIRFFARQSISSQFEVSLMDLARLSLRYRPDYLVIGEIRGKEIEALVHASASGHG
SLATFHAGNPHEALTRLISLLNKDVAKLFLQNLWGIIVLGTLRDRNGNIRRIIRSIYEIVYVKGKLRFKPVLKWSFSSNT
FLPNSVNDLVKKSYRINYISKTYEIPISEITEEISNRVKILDKLVNNEVLDLEDFSIYLKKITQGGQVETKAI

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=140, Percent_Identity=33.5714285714286, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786296, Length=212, Percent_Identity=27.3584905660377, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54035; Mature: 54035

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILQEYSVLNAKVIIYEDNGIGYYKVSEPSLDQKEIELLNSILEYIYSTPSISNIEETV
CCHHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VKILKNKGITDQGIIEKMIYYLRKRLGYDELTVPLADPYVEEVECKGFSYPITVVHRIVT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCHHHHHHHHHH
RFPRLYTNIVFEQEDHVLKVIEKLANKADKPVSIAKPYLEFSLPEGHRVAATVSREVSLP
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHCHHHEECCCCCCEEEEEEEEEEECC
GSTFDIRKFPLKPISPILLIKNVSISSLMLAYIWFLLDYKPFFLVIGSTGSGKTTLLNSI
CCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
LSMVNPFFKVITIEDTPEINIVHDNWIRFFARQSISSQFEVSLMDLARLSLRYRPDYLVI
HHHHCCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCEEEE
GEIRGKEIEALVHASASGHGSLATFHAGNPHEALTRLISLLNKDVAKLFLQNLWGIIVLG
ECCCCCHHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
TLRDRNGNIRRIIRSIYEIVYVKGKLRFKPVLKWSFSSNTFLPNSVNDLVKKSYRINYIS
EEECCCCCHHHHHHHHHHHHEEECCEEEEEEEEEECCCCCCCCCCHHHHHHHHHEEEEEC
KTYEIPISEITEEISNRVKILDKLVNNEVLDLEDFSIYLKKITQGGQVETKAI
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MNILQEYSVLNAKVIIYEDNGIGYYKVSEPSLDQKEIELLNSILEYIYSTPSISNIEETV
CCHHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VKILKNKGITDQGIIEKMIYYLRKRLGYDELTVPLADPYVEEVECKGFSYPITVVHRIVT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCHHHHHHHHHH
RFPRLYTNIVFEQEDHVLKVIEKLANKADKPVSIAKPYLEFSLPEGHRVAATVSREVSLP
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHCHHHEECCCCCCEEEEEEEEEEECC
GSTFDIRKFPLKPISPILLIKNVSISSLMLAYIWFLLDYKPFFLVIGSTGSGKTTLLNSI
CCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
LSMVNPFFKVITIEDTPEINIVHDNWIRFFARQSISSQFEVSLMDLARLSLRYRPDYLVI
HHHHCCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCEEEE
GEIRGKEIEALVHASASGHGSLATFHAGNPHEALTRLISLLNKDVAKLFLQNLWGIIVLG
ECCCCCHHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
TLRDRNGNIRRIIRSIYEIVYVKGKLRFKPVLKWSFSSNTFLPNSVNDLVKKSYRINYIS
EEECCCCCHHHHHHHHHHHHEEECCEEEEEEEEEECCCCCCCCCCHHHHHHHHHEEEEEC
KTYEIPISEITEEISNRVKILDKLVNNEVLDLEDFSIYLKKITQGGQVETKAI
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]