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The map label for this gene is ilvB1 [H]

Identifier: 227828230

GI number: 227828230

Start: 1805630

End: 1807261

Strand: Direct

Name: ilvB1 [H]

Synonym: M1425_1968

Alternate gene names: 227828230

Gene position: 1805630-1807261 (Clockwise)

Preceding gene: 227828228

Following gene: 227828231

Centisome position: 69.21

GC content: 37.01

Gene sequence:

>1632_bases
ATGAGTCAGCCCAAAAGGAAAGAGGAGACTGTAGGCGTAGAAATGAAAGGTGAGGAAGCACTTGCATATGTTCTTAATGA
CATTGGGATAACCAAAGTGTTTTCGACTTACTTCCTACCGAATATGGTTAAGGAAATGCTAAAGAAGTATAATATTGAAG
TAGAATTTTCTATGGCAGTAAAAGACGCAAGTTGGTTGGCATACGCTTATGCAATGGAAAATAACTCAGTCGGAACTATA
ATTCAGATACCTGGTTCTAAGCTAACTGATGCGGTAAATGTCATAGCTCAAGCATATATGGAATCGGTTCCTCTTCTTAT
TATATCTAGCGTAAGATCACATAAGGATACGGGAAGGGCTAGAATTGGCGAATTTAGAACGATTGATGACTTATCAAATA
TTTTATCTCCAATAACCAAGACAAAAGAAAGAGTTATCAGTATAGAAGAAATAACTGTTACAATAGAGAAAGCCTACAAG
GAAGCGGTTAGTAATAGACCAAGGCCTTCTTATGTTGAAATATCTGAAGACTTATTTAGGGCAAAAGCTTATCCGTTATC
CACTGCGGGTCAAAAGCCCGAGAAGAGGACTCCAGATAAAAATACTGTGGCTAAAGTGGCTGAACTTCTGACTAATGCTA
AATTACCAGTTATAATCGCGGGATATGGGATAGTTTTAAGCGATGCAGAAGACATGTTAGTTGAATTGGCTGAGTTATTA
GATTCACCGGTGGTTACTACATTTAAGGCGAAAGGTTCAATACCATCTAACCATAAATTATTTGCTGGAGAAGGATTAGG
CGCCTTTGGTACTGATGCTGCAAATTATTTGATAGAGAATGCTGACGTAATATTAGCTTTAGGCACTAGGTTTACTCAGT
TAAGTACGGCTGGCTGGTCATTAAAGTACAAGGGCATATTAGCACATAATAATGTTGATGGTGAAGACATAGGCAAGGTC
TTCATGCCTCATGTTCCAGTAGTAGCTGATACTGGGCTATTCTTAAGGGAGCTCTTAACACAATTGAAAGCTAAGATAAA
GGAGAAAGTAAATAGAGGAGCTAGTGACATTATTTATAAGAATCAGCGTGAAGTACATTCAGTTAAATCTCATAGCGGAT
TATGGCCAATTGACGTAGTGAAAATGCTAAGCACAGTAGGTGGTTTTGAGAAAGTGTATGTAGATATGTCAGCTACTACA
ATAGATTTCGTTAGATTACCAATAAACGCTAAGAAGAGCTGGTATACTGCAGAATCTTTGCTTGAGAGGGGTATTGCAGT
GGGTGGTGCTATAGCTTCTAAATACGTTGCATATGGAATTACTGACCTAGAAGGAATATTACCATATTTGTCATTACTAG
AATACAAAGTGGATAAGATTAAGGGAAAAATAATTGTGTTAAACGATGGTGGTGCAAATTATATTGAAGTTTCAAGCTCT
GATTTACCGACCATTACTAGATCACAAACTAGTATCAACGTTAATTTCAATGAGATTGTAAAAAGAACCCTAGGTGGTGT
AACTGTTGAAACGCTAACTGAGTTAGAGGAGGCTTTAAAATCTGCAGATAAGAAAATCGTAAATGTAAAGATAGATCCTA
ATTTCGAGTCAGTTGTAGTTTCAAGAATTTAA

Upstream 100 bases:

>100_bases
GGGCCGCAGTGGTATTAGTTGACACCCGTTATTTCGCGGGTTCAAATCCCGCCCCCGGCTTTTAGAAGATTTAAAAAGTA
GTCTTAATTATAATGTTAGT

Downstream 100 bases:

>100_bases
TATAAAGGTAAAGTACTTATTAAGACTAAAATTTTATTTTGTCTTATTCTGAAGTTTAAAATATGAACGATAAGTCTTCA
GATCAAATTTCAGTTAAGAA

Product: thiamine pyrophosphate protein central region

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSQPKRKEETVGVEMKGEEALAYVLNDIGITKVFSTYFLPNMVKEMLKKYNIEVEFSMAVKDASWLAYAYAMENNSVGTI
IQIPGSKLTDAVNVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSNILSPITKTKERVISIEEITVTIEKAYK
EAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNTVAKVAELLTNAKLPVIIAGYGIVLSDAEDMLVELAELL
DSPVVTTFKAKGSIPSNHKLFAGEGLGAFGTDAANYLIENADVILALGTRFTQLSTAGWSLKYKGILAHNNVDGEDIGKV
FMPHVPVVADTGLFLRELLTQLKAKIKEKVNRGASDIIYKNQREVHSVKSHSGLWPIDVVKMLSTVGGFEKVYVDMSATT
IDFVRLPINAKKSWYTAESLLERGIAVGGAIASKYVAYGITDLEGILPYLSLLEYKVDKIKGKIIVLNDGGANYIEVSSS
DLPTITRSQTSINVNFNEIVKRTLGGVTVETLTELEEALKSADKKIVNVKIDPNFESVVVSRI

Sequences:

>Translated_543_residues
MSQPKRKEETVGVEMKGEEALAYVLNDIGITKVFSTYFLPNMVKEMLKKYNIEVEFSMAVKDASWLAYAYAMENNSVGTI
IQIPGSKLTDAVNVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSNILSPITKTKERVISIEEITVTIEKAYK
EAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNTVAKVAELLTNAKLPVIIAGYGIVLSDAEDMLVELAELL
DSPVVTTFKAKGSIPSNHKLFAGEGLGAFGTDAANYLIENADVILALGTRFTQLSTAGWSLKYKGILAHNNVDGEDIGKV
FMPHVPVVADTGLFLRELLTQLKAKIKEKVNRGASDIIYKNQREVHSVKSHSGLWPIDVVKMLSTVGGFEKVYVDMSATT
IDFVRLPINAKKSWYTAESLLERGIAVGGAIASKYVAYGITDLEGILPYLSLLEYKVDKIKGKIIVLNDGGANYIEVSSS
DLPTITRSQTSINVNFNEIVKRTLGGVTVETLTELEEALKSADKKIVNVKIDPNFESVVVSRI
>Mature_542_residues
SQPKRKEETVGVEMKGEEALAYVLNDIGITKVFSTYFLPNMVKEMLKKYNIEVEFSMAVKDASWLAYAYAMENNSVGTII
QIPGSKLTDAVNVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSNILSPITKTKERVISIEEITVTIEKAYKE
AVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNTVAKVAELLTNAKLPVIIAGYGIVLSDAEDMLVELAELLD
SPVVTTFKAKGSIPSNHKLFAGEGLGAFGTDAANYLIENADVILALGTRFTQLSTAGWSLKYKGILAHNNVDGEDIGKVF
MPHVPVVADTGLFLRELLTQLKAKIKEKVNRGASDIIYKNQREVHSVKSHSGLWPIDVVKMLSTVGGFEKVYVDMSATTI
DFVRLPINAKKSWYTAESLLERGIAVGGAIASKYVAYGITDLEGILPYLSLLEYKVDKIKGKIIVLNDGGANYIEVSSSD
LPTITRSQTSINVNFNEIVKRTLGGVTVETLTELEEALKSADKKIVNVKIDPNFESVVVSRI

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=299, Percent_Identity=26.7558528428094, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI87081685, Length=285, Percent_Identity=29.4736842105263, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790104, Length=392, Percent_Identity=25.2551020408163, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786717, Length=333, Percent_Identity=26.1261261261261, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788716, Length=507, Percent_Identity=23.4714003944773, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=354, Percent_Identity=25.7062146892655, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=524, Percent_Identity=22.5190839694656, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922626, Length=529, Percent_Identity=22.4952741020794, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59608; Mature: 59477

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPKRKEETVGVEMKGEEALAYVLNDIGITKVFSTYFLPNMVKEMLKKYNIEVEFSMAV
CCCCCCCHHHCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
KDASWLAYAYAMENNSVGTIIQIPGSKLTDAVNVIAQAYMESVPLLIISSVRSHKDTGRA
CCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCH
RIGEFRTIDDLSNILSPITKTKERVISIEEITVTIEKAYKEAVSNRPRPSYVEISEDLFR
HCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHEEHHHHHHHHHHCCCCCCCEEHHHHHHHH
AKAYPLSTAGQKPEKRTPDKNTVAKVAELLTNAKLPVIIAGYGIVLSDAEDMLVELAELL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCHHHHHHHHHHHH
DSPVVTTFKAKGSIPSNHKLFAGEGLGAFGTDAANYLIENADVILALGTRFTQLSTAGWS
CCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCHHEEECCCCE
LKYKGILAHNNVDGEDIGKVFMPHVPVVADTGLFLRELLTQLKAKIKEKVNRGASDIIYK
EEEEEEEEECCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
NQREVHSVKSHSGLWPIDVVKMLSTVGGFEKVYVDMSATTIDFVRLPINAKKSWYTAESL
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHH
LERGIAVGGAIASKYVAYGITDLEGILPYLSLLEYKVDKIKGKIIVLNDGGANYIEVSSS
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCC
DLPTITRSQTSINVNFNEIVKRTLGGVTVETLTELEEALKSADKKIVNVKIDPNFESVVV
CCCCEECCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHE
SRI
ECC
>Mature Secondary Structure 
SQPKRKEETVGVEMKGEEALAYVLNDIGITKVFSTYFLPNMVKEMLKKYNIEVEFSMAV
CCCCCCHHHCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
KDASWLAYAYAMENNSVGTIIQIPGSKLTDAVNVIAQAYMESVPLLIISSVRSHKDTGRA
CCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCH
RIGEFRTIDDLSNILSPITKTKERVISIEEITVTIEKAYKEAVSNRPRPSYVEISEDLFR
HCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHEEHHHHHHHHHHCCCCCCCEEHHHHHHHH
AKAYPLSTAGQKPEKRTPDKNTVAKVAELLTNAKLPVIIAGYGIVLSDAEDMLVELAELL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCHHHHHHHHHHHH
DSPVVTTFKAKGSIPSNHKLFAGEGLGAFGTDAANYLIENADVILALGTRFTQLSTAGWS
CCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCHHEEECCCCE
LKYKGILAHNNVDGEDIGKVFMPHVPVVADTGLFLRELLTQLKAKIKEKVNRGASDIIYK
EEEEEEEEECCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
NQREVHSVKSHSGLWPIDVVKMLSTVGGFEKVYVDMSATTIDFVRLPINAKKSWYTAESL
CCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHH
LERGIAVGGAIASKYVAYGITDLEGILPYLSLLEYKVDKIKGKIIVLNDGGANYIEVSSS
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCC
DLPTITRSQTSINVNFNEIVKRTLGGVTVETLTELEEALKSADKKIVNVKIDPNFESVVV
CCCCEECCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHE
SRI
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]