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The map label for this gene is truD [H]

Identifier: 227828225

GI number: 227828225

Start: 1801675

End: 1802808

Strand: Direct

Name: truD [H]

Synonym: M1425_1963

Alternate gene names: 227828225

Gene position: 1801675-1802808 (Clockwise)

Preceding gene: 227828224

Following gene: 227828228

Centisome position: 69.06

GC content: 29.81

Gene sequence:

>1134_bases
GTGATCCCTCACGAAATAGACATTGCGTTAGGTATGGAAAGATATTACTATGAAGATTGGAACGAGATAGACGGAACAAT
CGAAAGACCAAATGGATTTAAAGTTGTAGAAGAGATTGACTTTAAGCCAGCTCACGAGTGGAAAGGTGAGGAAAAAGGTA
AATATGCAGTTTACTTGCTCACAAAATGGGGAATAGATCATTTTTCCGTTTTATCTGAAATTCAGAAAGTACTACATTCG
AAAGTGAATTATATGGGTATTAAGGATGCTAGCGCCATAACCTCTCAGTTAGTGTATATAACGTTAACCAAAAAACAAGA
ATTGATTAATGAGTATAAATCTAATAGTTTTATTCTAAAGTTTCTTGGATATTCTTCAATGAAGTTTAATCATACCGGGA
ATATATTTGAAATATCTTTGTCAACGTCAAATTTCGATTTAGTCGTAGAAAGAGTGAGACAAATTGAGAAAAATCCATAT
CTCCCCGCTTTTATAGGTTATCAACGTTTTGGTACTCGTAGGCCTGTCACACATTTAATAGGTAAATATCTATTGAAAAG
AGACTGGGAAAAAGCATTCTATTTGATTTTGACGTATCCTTTTCTTTCTGAATCAAAAGAGATTATTGAAATTAGAAAGC
TAATAATAAAAGGTGATTTTAATGAAGCAGTTAGATTGATTCCGTCTAAGTTTAAACAAGAGAAACTATTATTGAAAAAC
TACATGAAATTTAATTCTTATTACCTAGCTCTTAAAAATAGTTTCATTCCAATCTCGTTATATTTAGATGCATATCAAAG
TTATTTATTTAACATGTATTTATCCAGAAGGTTAGACAAGTATAAGAACTCAGATGATAAGGATAATTTATTTATAAGAA
TTCCTATCTATTTTAATGACTGTGATGATATTTGCAAGGAGATTTATCTGAATGAAGAAATAGAAAAGAATTTTTTTAAA
TTACCAGAATTTAAAATTTCACTAAAAAGCCTCAATAGAAAGGCATTTATGAATATAAGAAATTTAAAAATTAACGAGGA
AACAAGAACAATATCTTTTGTATTAGATAGGGGGATGTACGCTACAGTATTACTTAGGGAAATTTTAAGAGGAGATCCAA
GAAAATTCACTTAA

Upstream 100 bases:

>100_bases
CAGGAAAGACCCAATTAGAGCCTGGAACCATAACTTGTCTAGGTATTGGACCAGCTCCAGAGAATTTGATAGATCCTATC
ACGGGTGATCTAAAATTACT

Downstream 100 bases:

>100_bases
CTTTTATGGTGTTAGTCTCTTCTTATCTCTAGGGAATGGTACTACTTCCTTTACGCTCTGAAGTCCAGTTAACATTAACA
TTACTCTTGCTAATCCCATG

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIPHEIDIALGMERYYYEDWNEIDGTIERPNGFKVVEEIDFKPAHEWKGEEKGKYAVYLLTKWGIDHFSVLSEIQKVLHS
KVNYMGIKDASAITSQLVYITLTKKQELINEYKSNSFILKFLGYSSMKFNHTGNIFEISLSTSNFDLVVERVRQIEKNPY
LPAFIGYQRFGTRRPVTHLIGKYLLKRDWEKAFYLILTYPFLSESKEIIEIRKLIIKGDFNEAVRLIPSKFKQEKLLLKN
YMKFNSYYLALKNSFIPISLYLDAYQSYLFNMYLSRRLDKYKNSDDKDNLFIRIPIYFNDCDDICKEIYLNEEIEKNFFK
LPEFKISLKSLNRKAFMNIRNLKINEETRTISFVLDRGMYATVLLREILRGDPRKFT

Sequences:

>Translated_377_residues
MIPHEIDIALGMERYYYEDWNEIDGTIERPNGFKVVEEIDFKPAHEWKGEEKGKYAVYLLTKWGIDHFSVLSEIQKVLHS
KVNYMGIKDASAITSQLVYITLTKKQELINEYKSNSFILKFLGYSSMKFNHTGNIFEISLSTSNFDLVVERVRQIEKNPY
LPAFIGYQRFGTRRPVTHLIGKYLLKRDWEKAFYLILTYPFLSESKEIIEIRKLIIKGDFNEAVRLIPSKFKQEKLLLKN
YMKFNSYYLALKNSFIPISLYLDAYQSYLFNMYLSRRLDKYKNSDDKDNLFIRIPIYFNDCDDICKEIYLNEEIEKNFFK
LPEFKISLKSLNRKAFMNIRNLKINEETRTISFVLDRGMYATVLLREILRGDPRKFT
>Mature_377_residues
MIPHEIDIALGMERYYYEDWNEIDGTIERPNGFKVVEEIDFKPAHEWKGEEKGKYAVYLLTKWGIDHFSVLSEIQKVLHS
KVNYMGIKDASAITSQLVYITLTKKQELINEYKSNSFILKFLGYSSMKFNHTGNIFEISLSTSNFDLVVERVRQIEKNPY
LPAFIGYQRFGTRRPVTHLIGKYLLKRDWEKAFYLILTYPFLSESKEIIEIRKLIIKGDFNEAVRLIPSKFKQEKLLLKN
YMKFNSYYLALKNSFIPISLYLDAYQSYLFNMYLSRRLDKYKNSDDKDNLFIRIPIYFNDCDDICKEIYLNEEIEKNFFK
LPEFKISLKSLNRKAFMNIRNLKINEETRTISFVLDRGMYATVLLREILRGDPRKFT

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI50727002, Length=253, Percent_Identity=28.0632411067194, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324817, Length=255, Percent_Identity=29.0196078431373, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 44884; Mature: 44884

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPHEIDIALGMERYYYEDWNEIDGTIERPNGFKVVEEIDFKPAHEWKGEEKGKYAVYLL
CCCCEEEHEECHHHHHHCCHHHCCCEEECCCCCEEHHHCCCCCCCCCCCCCCCCEEEEEE
TKWGIDHFSVLSEIQKVLHSKVNYMGIKDASAITSQLVYITLTKKQELINEYKSNSFILK
EECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCHHHHHHHHCCCCEEEE
FLGYSSMKFNHTGNIFEISLSTSNFDLVVERVRQIEKNPYLPAFIGYQRFGTRRPVTHLI
EECCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHH
GKYLLKRDWEKAFYLILTYPFLSESKEIIEIRKLIIKGDFNEAVRLIPSKFKQEKLLLKN
HHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YMKFNSYYLALKNSFIPISLYLDAYQSYLFNMYLSRRLDKYKNSDDKDNLFIRIPIYFND
HHHHCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECC
CDDICKEIYLNEEIEKNFFKLPEFKISLKSLNRKAFMNIRNLKINEETRTISFVLDRGMY
HHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCHHHHEEEECEEECCCHHEEEEHHHCCHH
ATVLLREILRGDPRKFT
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPHEIDIALGMERYYYEDWNEIDGTIERPNGFKVVEEIDFKPAHEWKGEEKGKYAVYLL
CCCCEEEHEECHHHHHHCCHHHCCCEEECCCCCEEHHHCCCCCCCCCCCCCCCCEEEEEE
TKWGIDHFSVLSEIQKVLHSKVNYMGIKDASAITSQLVYITLTKKQELINEYKSNSFILK
EECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCHHHHHHHHCCCCEEEE
FLGYSSMKFNHTGNIFEISLSTSNFDLVVERVRQIEKNPYLPAFIGYQRFGTRRPVTHLI
EECCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHH
GKYLLKRDWEKAFYLILTYPFLSESKEIIEIRKLIIKGDFNEAVRLIPSKFKQEKLLLKN
HHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YMKFNSYYLALKNSFIPISLYLDAYQSYLFNMYLSRRLDKYKNSDDKDNLFIRIPIYFND
HHHHCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECC
CDDICKEIYLNEEIEKNFFKLPEFKISLKSLNRKAFMNIRNLKINEETRTISFVLDRGMY
HHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCHHHHEEEECEEECCCHHEEEEHHHCCHH
ATVLLREILRGDPRKFT
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]