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Identifier: 227828207

GI number: 227828207

Start: 1784140

End: 1785945

Strand: Direct

Name: lig

Synonym: M1425_1945

Alternate gene names: 227828207

Gene position: 1784140-1785945 (Clockwise)

Preceding gene: 227828205

Following gene: 227828208

Centisome position: 68.39

GC content: 36.38

Gene sequence:

>1806_bases
ATGGAGTTTAAAGTTATTGCCGAATACTTTGATAAATTAGAGAAGATATCTTCAAGGCTCCAGCTTACAGCTTTACTGGC
TGATCTTTTAAGCAAAAGTGATAAGGCAATTATAGATAAGGTAGTTTATATTATACAAGGCAAATTATGGCCTGATTTTC
TAGGTTATCCAGAGCTTGGAATTGGAGAGAAATTCTTAATAAAAGCAATTAGTATAGCTACGAACACAGATGAAAATTCA
GTAGAAAATCTCTATAAGTCAATAGGTGATCTTGGAGAAGTAGCTCGAAGGTTGAAAAGTAAGCAGCAAAGTACAGGAAT
TTTAGGTTTTTTGGGTACTTCAAGTAAAGAGAGCTTAAAAGTGGATGAAGTTTACTCTACGTTATCTAAGGTGGCATTAA
CTACGGGAGAGGGTAGTAGAGATTTAAAGATAAGATTACTGGCAGGATTGTTAAAAAAGGCAGATCCGTTAGAGGCCAAG
TTCCTAGTTAGGTTTGTTGAAGGTCGACTTAGAGTTGGTATAGGAGATGCGACAGTTTTAGATGCTATGGCAATAGCTTT
TGGCGGGGGGCAATCAGCATCAGAAATAGTTGAAAGAGCTTATAATCTTAGGGCGGATCTAGGAAACATTGCAAAAATTA
TAGTAGAGAAGGGAATTGAAGCCTTAAAGACTCTAAAACCTGAAGTTGGAATACCAATTAGACCGATGCTAGCTGAAAGG
TTATCAAATCCTGAAGAAATATTAAAGAAAGTAGGTGGAAGTGCATTAGTCGATTATAAATACGATGGAGAGAGAGCCCA
AATACACAAAAAAGATGACAAAATTTTTATATTTTCTCGAAGATTAGAGAACATTACTTCTCAGTATCCAGATGTAGTAG
AGTATATTAGTAAATACACTGAAGGCAAAGAGTTCATTATAGAAGGAGAAATAGTGGCTGTAGATCCAGAAAGCGGTGAG
ATGAGATCATTTCAAGAATTAATGCACAGAAAGAGGAAGTCAGATATTTACGAAGCAATTAAAGAGTACCCAGTAAACGT
ATTTCTCTTTGATTTAATGTATTATGAAGATGTAGATTATACTACAAAGCCATTAGAAGTTAGGAGAAAATTGTTAGAAA
GTATTGTAAAACCAAACGACTATGTAAAAATAGCTCATCATATTCAAGTTAATAATGTAGAGGATCTTAAATCGTTCTTC
TATCGAGCAATATCTGAGGGTGGAGAGGGAGTAATGGTGAAGGCAATTGGAAAAGATGCAATATATCAAGCCGGTGCTAG
GGGTTGGTTATGGATAAAGCTTAAACGTGATTATCAAAGCGAGATGGCTGATACAGTAGACTTAGTAGTTGTTGGTGGTT
TCTATGGGAAGGGTAAAAGAGGCGGCAAGATAAGCTCACTATTAATGGCCGCATATAATCCTAAGACTGATACTTTCGAA
TCTGTTTGCAAGGTGGCTAGTGGCTTCTCAGATGAGCAACTTGATGAGTTACAGAAGAAGTTAATGGAAATAAAGAGGGA
TATAAAACATCCTAGAGTTAATTCTAAGATGGAACCAGATATATGGGTAGAGCCAGTTTATGTAGCGGAAATAATTGGAG
CGGAGATAACAATATCTCCTTTACATACTTGTTGTCAAGACGTCGTAGAGAAGGATGCTGGTTTATCTATAAGATTTCCT
CGATTTATAAGATGGAGAGATGATAAAAGTCCAGAAGATGCTACAACCACTGATGAGATACTTGAAATGTACAACAAGCA
GCCTAAGAAGAAGATCGAATCACCACCTATAGACGAGAGCGTTTAA

Upstream 100 bases:

>100_bases
GTTCAGAGTAACTCTGACTCTTCATCTGAAGTCGGTAATTGCCGAGTATTCATCATCCATGAAATGATTATAAATAACTG
CAGATAATATAAATTAATCT

Downstream 100 bases:

>100_bases
AATATATCGGGGCTCTACATATGGTATTAATGTGCAGAATCTTGTAAAGATACTTCCTAATGGGGCTATTTTAATCGGTG
AAAAATTTACTATTGATGGA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MEFKVIAEYFDKLEKISSRLQLTALLADLLSKSDKAIIDKVVYIIQGKLWPDFLGYPELGIGEKFLIKAISIATNTDENS
VENLYKSIGDLGEVARRLKSKQQSTGILGFLGTSSKESLKVDEVYSTLSKVALTTGEGSRDLKIRLLAGLLKKADPLEAK
FLVRFVEGRLRVGIGDATVLDAMAIAFGGGQSASEIVERAYNLRADLGNIAKIIVEKGIEALKTLKPEVGIPIRPMLAER
LSNPEEILKKVGGSALVDYKYDGERAQIHKKDDKIFIFSRRLENITSQYPDVVEYISKYTEGKEFIIEGEIVAVDPESGE
MRSFQELMHRKRKSDIYEAIKEYPVNVFLFDLMYYEDVDYTTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFF
YRAISEGGEGVMVKAIGKDAIYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYNPKTDTFE
SVCKVASGFSDEQLDELQKKLMEIKRDIKHPRVNSKMEPDIWVEPVYVAEIIGAEITISPLHTCCQDVVEKDAGLSIRFP
RFIRWRDDKSPEDATTTDEILEMYNKQPKKKIESPPIDESV

Sequences:

>Translated_601_residues
MEFKVIAEYFDKLEKISSRLQLTALLADLLSKSDKAIIDKVVYIIQGKLWPDFLGYPELGIGEKFLIKAISIATNTDENS
VENLYKSIGDLGEVARRLKSKQQSTGILGFLGTSSKESLKVDEVYSTLSKVALTTGEGSRDLKIRLLAGLLKKADPLEAK
FLVRFVEGRLRVGIGDATVLDAMAIAFGGGQSASEIVERAYNLRADLGNIAKIIVEKGIEALKTLKPEVGIPIRPMLAER
LSNPEEILKKVGGSALVDYKYDGERAQIHKKDDKIFIFSRRLENITSQYPDVVEYISKYTEGKEFIIEGEIVAVDPESGE
MRSFQELMHRKRKSDIYEAIKEYPVNVFLFDLMYYEDVDYTTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFF
YRAISEGGEGVMVKAIGKDAIYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYNPKTDTFE
SVCKVASGFSDEQLDELQKKLMEIKRDIKHPRVNSKMEPDIWVEPVYVAEIIGAEITISPLHTCCQDVVEKDAGLSIRFP
RFIRWRDDKSPEDATTTDEILEMYNKQPKKKIESPPIDESV
>Mature_601_residues
MEFKVIAEYFDKLEKISSRLQLTALLADLLSKSDKAIIDKVVYIIQGKLWPDFLGYPELGIGEKFLIKAISIATNTDENS
VENLYKSIGDLGEVARRLKSKQQSTGILGFLGTSSKESLKVDEVYSTLSKVALTTGEGSRDLKIRLLAGLLKKADPLEAK
FLVRFVEGRLRVGIGDATVLDAMAIAFGGGQSASEIVERAYNLRADLGNIAKIIVEKGIEALKTLKPEVGIPIRPMLAER
LSNPEEILKKVGGSALVDYKYDGERAQIHKKDDKIFIFSRRLENITSQYPDVVEYISKYTEGKEFIIEGEIVAVDPESGE
MRSFQELMHRKRKSDIYEAIKEYPVNVFLFDLMYYEDVDYTTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFF
YRAISEGGEGVMVKAIGKDAIYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYNPKTDTFE
SVCKVASGFSDEQLDELQKKLMEIKRDIKHPRVNSKMEPDIWVEPVYVAEIIGAEITISPLHTCCQDVVEKDAGLSIRFP
RFIRWRDDKSPEDATTTDEILEMYNKQPKKKIESPPIDESV

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=623, Percent_Identity=33.2263242375602, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI73747829, Length=595, Percent_Identity=30.5882352941176, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI73747844, Length=595, Percent_Identity=30.5882352941176, Blast_Score=213, Evalue=5e-55,
Organism=Homo sapiens, GI46255052, Length=495, Percent_Identity=26.0606060606061, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI23199993, Length=495, Percent_Identity=26.0606060606061, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI148539894, Length=495, Percent_Identity=26.0606060606061, Blast_Score=148, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71985269, Length=613, Percent_Identity=35.0734094616639, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI71985278, Length=150, Percent_Identity=32, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71981465, Length=379, Percent_Identity=24.2744063324538, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320038, Length=617, Percent_Identity=34.0356564019449, Blast_Score=323, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6324578, Length=401, Percent_Identity=25.9351620947631, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24762424, Length=624, Percent_Identity=33.0128205128205, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI85815841, Length=563, Percent_Identity=30.1953818827709, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_SULIA (C3MZR1)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843938.1
- GeneID:   7812129
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_2023
- OMA:   EYQSICK
- ProtClustDB:   PRK01109
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 67717; Mature: 67717

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 260-260 BINDING 258-258 BINDING 265-265 BINDING 280-280 BINDING 310-310 BINDING 350-350 BINDING 427-427 BINDING 433-433

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKVIAEYFDKLEKISSRLQLTALLADLLSKSDKAIIDKVVYIIQGKLWPDFLGYPELG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCCCCCC
IGEKFLIKAISIATNTDENSVENLYKSIGDLGEVARRLKSKQQSTGILGFLGTSSKESLK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
VDEVYSTLSKVALTTGEGSRDLKIRLLAGLLKKADPLEAKFLVRFVEGRLRVGIGDATVL
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHEECCCHHHHH
DAMAIAFGGGQSASEIVERAYNLRADLGNIAKIIVEKGIEALKTLKPEVGIPIRPMLAER
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LSNPEEILKKVGGSALVDYKYDGERAQIHKKDDKIFIFSRRLENITSQYPDVVEYISKYT
CCCHHHHHHHCCCCEEEEEEECCCHHEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHHHC
EGKEFIIEGEIVAVDPESGEMRSFQELMHRKRKSDIYEAIKEYPVNVFLFDLMYYEDVDY
CCCEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCC
TTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFFYRAISEGGEGVMVKAIGKDA
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHH
IYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYNPKTDTFE
HHHCCCCCEEEEEEECHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHH
SVCKVASGFSDEQLDELQKKLMEIKRDIKHPRVNSKMEPDIWVEPVYVAEIIGAEITISP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHCCCEEEHH
LHTCCQDVVEKDAGLSIRFPRFIRWRDDKSPEDATTTDEILEMYNKQPKKKIESPPIDES
HHHHHHHHHHCCCCCEEECCHHEEECCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCC
V
C
>Mature Secondary Structure
MEFKVIAEYFDKLEKISSRLQLTALLADLLSKSDKAIIDKVVYIIQGKLWPDFLGYPELG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCCCCCC
IGEKFLIKAISIATNTDENSVENLYKSIGDLGEVARRLKSKQQSTGILGFLGTSSKESLK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
VDEVYSTLSKVALTTGEGSRDLKIRLLAGLLKKADPLEAKFLVRFVEGRLRVGIGDATVL
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHEECCCHHHHH
DAMAIAFGGGQSASEIVERAYNLRADLGNIAKIIVEKGIEALKTLKPEVGIPIRPMLAER
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LSNPEEILKKVGGSALVDYKYDGERAQIHKKDDKIFIFSRRLENITSQYPDVVEYISKYT
CCCHHHHHHHCCCCEEEEEEECCCHHEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHHHC
EGKEFIIEGEIVAVDPESGEMRSFQELMHRKRKSDIYEAIKEYPVNVFLFDLMYYEDVDY
CCCEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCC
TTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFFYRAISEGGEGVMVKAIGKDA
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHH
IYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYNPKTDTFE
HHHCCCCCEEEEEEECHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHH
SVCKVASGFSDEQLDELQKKLMEIKRDIKHPRVNSKMEPDIWVEPVYVAEIIGAEITISP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHCCCEEEHH
LHTCCQDVVEKDAGLSIRFPRFIRWRDDKSPEDATTTDEILEMYNKQPKKKIESPPIDES
HHHHHHHHHHCCCCCEEECCHHEEECCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA