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The map label for this gene is rpoA2

Identifier: 227828175

GI number: 227828175

Start: 1756832

End: 1758010

Strand: Direct

Name: rpoA2

Synonym: M1425_1913

Alternate gene names: 227828175

Gene position: 1756832-1758010 (Clockwise)

Preceding gene: 227828174

Following gene: 227828176

Centisome position: 67.34

GC content: 34.78

Gene sequence:

>1179_bases
GTGATTGATGAAAAGGATAAATCTTATTTAGAAGAGAAAGTGAAACAAGCTTCCAACATTCTTCCTCAAAAAATTGTGGA
AGATTTGAAAAATTTGATATCTAACAAGGAAGTACTGGTTACTAGAGATGAAATTGACAAAATCTTCGATTTGGCTATTA
AAGAGTATAGTGAAGGACTAATAGCTCCAGGAGAGGCTATTGGAATTGTCGCCGCACAATCAGTAGGAGAGCCAGGTACA
CAAATGACATTAAGGACTTTTCATTTTGCGGGTATAAGAGAGTTGAACGTAACGTTAGGCCTTCCAAGACTAATAGAAAT
TGTAGACGCAAAAAAAGTTCCATCAACTCCAATGATGACCATTTATTTGACTGATGAATACAAACACGATAAGGAAAAAG
CGTTAGAAGTTGCTAGAAAATTAGAGTATACAAAAATAGAGAATGTAGTTAGTTCAACAAGTATTGATATAGCTTCAATG
TCGATTATTCTCCAACTTGATAATGAAATGCTTAAAGACAAAGGCGTTACTGTAGACGACGTTAAAAAAGCTATAAATAG
ATTGAAATTAGGAGAATTTGTGATAGATGAATCTGAGGGTAATACATTAAATATAAGTTTCGCTAATATAGATAGTATAG
CTGCACTGTTTAAATTAAGGGATAAGATACTTAATACTAAAATAAAGGGAATAAAGGGAATAAAACGTGCAATAGTTCAG
AAAAAAGGCGATGAATATATTATTCTAACAGATGGTTCAAATCTATCTGGTGTTCTAAGTGTGAAAGGAGTTGATATTGC
CAAAGTAGAAACTAATAATATTCGTGAGATCGAGGAGGTATTTGGAATAGAGGCTGCAAGGGAAATTATAATTAGAGAAA
TTAGTAAAGTATTAGCAGAACAAGGTTTGGATGTTGACATGAGACACATATTGCTGGTTGCGGATGTGATGACTAGAACA
GGTGTCGTAAGACAGATAGGTAGGCATGGTGTAACTGGGGAGAAGAATAGTGTATTGGCAAGAGCCGCATTTGAGGTTAC
TGTAAAACACCTTTTGGATGCTGCAGCTAGAGGAGATGTAGAAGAATTTAAAGGTGTAGTAGAAAACATTATAATTGGCC
ATCCGATTAAACTAGGTACCGGAATGGTTGAATTAACAATGAGGCCGATATTAAGGTGA

Upstream 100 bases:

>100_bases
AAGTAGCGTACGGCGATGATGGTGTATTTCCCATGTATTCAGCTCATGGTAAAACGGTAGATGTAAACAGGATATTTGAA
AGAGTCGTGGGGTGGAAAAC

Downstream 100 bases:

>100_bases
TAAAAAGTGTCAGAACAACAAGTTTCATTTGAAGGAGAGTTGAAATCTCTTTTAAAGACGGGTAAAGTTATTTTTGGTGC
CAAGAGAGCTATAAAATACA

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIDEKDKSYLEEKVKQASNILPQKIVEDLKNLISNKEVLVTRDEIDKIFDLAIKEYSEGLIAPGEAIGIVAAQSVGEPGT
QMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMTIYLTDEYKHDKEKALEVARKLEYTKIENVVSSTSIDIASM
SIILQLDNEMLKDKGVTVDDVKKAINRLKLGEFVIDESEGNTLNISFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQ
KKGDEYIILTDGSNLSGVLSVKGVDIAKVETNNIREIEEVFGIEAAREIIIREISKVLAEQGLDVDMRHILLVADVMTRT
GVVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDVEEFKGVVENIIIGHPIKLGTGMVELTMRPILR

Sequences:

>Translated_392_residues
MIDEKDKSYLEEKVKQASNILPQKIVEDLKNLISNKEVLVTRDEIDKIFDLAIKEYSEGLIAPGEAIGIVAAQSVGEPGT
QMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMTIYLTDEYKHDKEKALEVARKLEYTKIENVVSSTSIDIASM
SIILQLDNEMLKDKGVTVDDVKKAINRLKLGEFVIDESEGNTLNISFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQ
KKGDEYIILTDGSNLSGVLSVKGVDIAKVETNNIREIEEVFGIEAAREIIIREISKVLAEQGLDVDMRHILLVADVMTRT
GVVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDVEEFKGVVENIIIGHPIKLGTGMVELTMRPILR
>Mature_392_residues
MIDEKDKSYLEEKVKQASNILPQKIVEDLKNLISNKEVLVTRDEIDKIFDLAIKEYSEGLIAPGEAIGIVAAQSVGEPGT
QMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMTIYLTDEYKHDKEKALEVARKLEYTKIENVVSSTSIDIASM
SIILQLDNEMLKDKGVTVDDVKKAINRLKLGEFVIDESEGNTLNISFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQ
KKGDEYIILTDGSNLSGVLSVKGVDIAKVETNNIREIEEVFGIEAAREIIIREISKVLAEQGLDVDMRHILLVADVMTRT
GVVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDVEEFKGVVENIIIGHPIKLGTGMVELTMRPILR

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family

Homologues:

Organism=Homo sapiens, GI39725938, Length=362, Percent_Identity=34.2541436464088, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI4505939, Length=402, Percent_Identity=32.3383084577114, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI103471997, Length=99, Percent_Identity=46.4646464646465, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25145495, Length=393, Percent_Identity=33.8422391857506, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI71987878, Length=434, Percent_Identity=31.5668202764977, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI71998295, Length=105, Percent_Identity=45.7142857142857, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320061, Length=411, Percent_Identity=30.4136253041363, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324690, Length=402, Percent_Identity=33.8308457711443, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324917, Length=73, Percent_Identity=58.9041095890411, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17530899, Length=394, Percent_Identity=34.010152284264, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI281360912, Length=344, Percent_Identity=36.6279069767442, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI17647875, Length=160, Percent_Identity=33.75, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOA2_SULIA (C3MZM9)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843906.1
- GeneID:   7813071
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1990
- OMA:   LVADMMT
- ProtClustDB:   PRK04309
- HAMAP:   MF_00411
- InterPro:   IPR007081
- InterPro:   IPR012757
- PANTHER:   PTHR19376:SF13
- TIGRFAMs:   TIGR02389

Pfam domain/function: PF04998 RNA_pol_Rpb1_5

EC number: =2.7.7.6

Molecular weight: Translated: 43390; Mature: 43390

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDEKDKSYLEEKVKQASNILPQKIVEDLKNLISNKEVLVTRDEIDKIFDLAIKEYSEGL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCC
IAPGEAIGIVAAQSVGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMT
CCCCCHHEEEEHHCCCCCCCCEEEHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCEEE
IYLTDEYKHDKEKALEVARKLEYTKIENVVSSTSIDIASMSIILQLDNEMLKDKGVTVDD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECHHHHHHCCCCHHH
VKKAINRLKLGEFVIDESEGNTLNISFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQ
HHHHHHHHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGDEYIILTDGSNLSGVLSVKGVDIAKVETNNIREIEEVFGIEAAREIIIREISKVLAE
CCCCCEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLDVDMRHILLVADVMTRTGVVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
EEFKGVVENIIIGHPIKLGTGMVELTMRPILR
HHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MIDEKDKSYLEEKVKQASNILPQKIVEDLKNLISNKEVLVTRDEIDKIFDLAIKEYSEGL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCC
IAPGEAIGIVAAQSVGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMT
CCCCCHHEEEEHHCCCCCCCCEEEHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCEEE
IYLTDEYKHDKEKALEVARKLEYTKIENVVSSTSIDIASMSIILQLDNEMLKDKGVTVDD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECHHHHHHCCCCHHH
VKKAINRLKLGEFVIDESEGNTLNISFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQ
HHHHHHHHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGDEYIILTDGSNLSGVLSVKGVDIAKVETNNIREIEEVFGIEAAREIIIREISKVLAE
CCCCCEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLDVDMRHILLVADVMTRTGVVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
EEFKGVVENIIIGHPIKLGTGMVELTMRPILR
HHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA