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The map label for this gene is 227828163

Identifier: 227828163

GI number: 227828163

Start: 1742108

End: 1743856

Strand: Direct

Name: 227828163

Synonym: M1425_1901

Alternate gene names: NA

Gene position: 1742108-1743856 (Clockwise)

Preceding gene: 227828162

Following gene: 227828169

Centisome position: 66.78

GC content: 35.91

Gene sequence:

>1749_bases
ATGGCTCAGACAGTAAACACTGAAAGGCATTCGAGACTTTTGTCTGCACCGTCAAGTTTCGAGGAAGGAGTAGTCAAATT
TGGAGATAAAGAAATTAAGATAGGAGGACCCTTACCAACATTAGCTAAAGATGAGAAGTTAGTTAGGGTAACTCATTCAC
TGTGTCCAGCGTGCTATAGGCTTTTGCCAGCTACTATATTTGAAAAGGAAGGAAAATTATTCATAAGAAAAATATGCCCA
GAACATGGAGAATTTGAGGACTTATATTACGGAGATTCTAGTTTATACTATAAGTTCGACTACTGGGAGTATGAAGGAAA
AGGACCTAAAGTCCCCTATGTCGACTTGAAATCTCCTTGTCCTTATAACTGTGGATTATGTCCGATGCATCATCAACATT
CAGCATTGGTTAATTTAGTAATAACAAATAGATGTGACTTATCTTGTTGGTATTGTTTCTTCTATGCTGAAAAGGCAGGA
TATGTTTATGAACCTACGTTAGAGCAAATCAAGTTTATGGTAGATCAATTAAAGAAACAAGATATTACGCTGGTTATCCA
AGTTACTGGAGGAGAGCCTACATTAAGGGAGGATATAATAGAAATAGTAAAGATGCTTAGAGAGAACGGTGTAAAACATA
TTCAATTAAATAGTTGGGGAGGTACTTTTGCCAAGCTGTATTTAGAAGATCCCGATAAGGCGATTAAATACGCTAGAGCT
CTTAGAGAAGCTGGAGTGAATACTGTTTACATGAGTTTTGACGGTACTACTAGAAAAACTAATCCCAAGAATCATTGGGA
AATTCCATACACGTTAGAAGTATTTAGAAGAGCGGGAATGACAAGTGTTGTATTAGTTCCTACTGTAATTAAGACAGTTA
ATGATAATGATCTAGGTAATATAGTTAAGTTCGCAGCATACAATATGGATGTTGTTAGGTCAGTAAACTTCCAGCCAGTG
AGTTTAACAGGAATGATGAAAAGGAATATGAGAGCTAAATTCAGAATCACGATACCCGAAGTTATAAAGAATATAGAAGA
ACAAACTGATGGTGAGATAACTAAGGATAGTTGGTATCCGATAGGTACTTCTGTTGTCTTCTCAAGGCTAGTTGAAGCAT
TAACTGGTAAGGAGCAATTCGAAATGGCTAATCATCCTTCTTGTGGAGCTGGAACGTACGTGTATGTAGAATGGAGAAAC
CATGAACCTCACTTCATACCAATTTCGAAGTTTATTGACTTAGAAGGTTTGCTAGAATATCTTAAAGAGAAAGAAGAAGA
GTTAAGGGAAGGTGCTAACAAAGTATGGGTTGCAAGTAAAGTATTGTATAGTATAAGGAAGTTCATAGATAAAGAACAAG
GACCTAAGGACTTTGATGTATATAAGATGCTCTATAACATAATAATTCATCATAACTACGAAGCACTTGGAGAGTGGCAC
TACAGAACATTATTCTTAGGAACTATGCACTTCATGGACTTATATAACTACGATGTACAAAGAGTGATGAGATGTGATAT
TCACTATGTTGTTCCAGATGGTAGAGTAATCCCATTCTGTACCTATAATGTATTAAATGATTTGTATAGAGATAAAGTAC
TGAGAGAATATCAAATACCTCTTGATAAATGGACTAAGGAGCATGGGGAGAACTCATTAGGCGATGCTATAAAGTATAAA
CGTATGGCCTCAACCTTAGAGAATTCCGAGATCTATAAGAATACTTATAAATCCTTCCAACAAATGTAA

Upstream 100 bases:

>100_bases
AAAAATACTGGATGGGATAATTTTATAGGTTGGTATTAACTGAATGTACGTAAGTCAAAGGGAATATCTTTATATATAAA
GATCGGCAATCATACTTTCA

Downstream 100 bases:

>100_bases
CTTAATTTAATTAAAAATATTTTTCTAATGAAGTTTTTTCTTGTTTTCTAAACAGCTGTTTTTTATTTGGAATTAAATCT
TTGAACCAAACTCTTAGATT

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAQTVNTERHSRLLSAPSSFEEGVVKFGDKEIKIGGPLPTLAKDEKLVRVTHSLCPACYRLLPATIFEKEGKLFIRKICP
EHGEFEDLYYGDSSLYYKFDYWEYEGKGPKVPYVDLKSPCPYNCGLCPMHHQHSALVNLVITNRCDLSCWYCFFYAEKAG
YVYEPTLEQIKFMVDQLKKQDITLVIQVTGGEPTLREDIIEIVKMLRENGVKHIQLNSWGGTFAKLYLEDPDKAIKYARA
LREAGVNTVYMSFDGTTRKTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTVNDNDLGNIVKFAAYNMDVVRSVNFQPV
SLTGMMKRNMRAKFRITIPEVIKNIEEQTDGEITKDSWYPIGTSVVFSRLVEALTGKEQFEMANHPSCGAGTYVYVEWRN
HEPHFIPISKFIDLEGLLEYLKEKEEELREGANKVWVASKVLYSIRKFIDKEQGPKDFDVYKMLYNIIIHHNYEALGEWH
YRTLFLGTMHFMDLYNYDVQRVMRCDIHYVVPDGRVIPFCTYNVLNDLYRDKVLREYQIPLDKWTKEHGENSLGDAIKYK
RMASTLENSEIYKNTYKSFQQM

Sequences:

>Translated_582_residues
MAQTVNTERHSRLLSAPSSFEEGVVKFGDKEIKIGGPLPTLAKDEKLVRVTHSLCPACYRLLPATIFEKEGKLFIRKICP
EHGEFEDLYYGDSSLYYKFDYWEYEGKGPKVPYVDLKSPCPYNCGLCPMHHQHSALVNLVITNRCDLSCWYCFFYAEKAG
YVYEPTLEQIKFMVDQLKKQDITLVIQVTGGEPTLREDIIEIVKMLRENGVKHIQLNSWGGTFAKLYLEDPDKAIKYARA
LREAGVNTVYMSFDGTTRKTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTVNDNDLGNIVKFAAYNMDVVRSVNFQPV
SLTGMMKRNMRAKFRITIPEVIKNIEEQTDGEITKDSWYPIGTSVVFSRLVEALTGKEQFEMANHPSCGAGTYVYVEWRN
HEPHFIPISKFIDLEGLLEYLKEKEEELREGANKVWVASKVLYSIRKFIDKEQGPKDFDVYKMLYNIIIHHNYEALGEWH
YRTLFLGTMHFMDLYNYDVQRVMRCDIHYVVPDGRVIPFCTYNVLNDLYRDKVLREYQIPLDKWTKEHGENSLGDAIKYK
RMASTLENSEIYKNTYKSFQQM
>Mature_581_residues
AQTVNTERHSRLLSAPSSFEEGVVKFGDKEIKIGGPLPTLAKDEKLVRVTHSLCPACYRLLPATIFEKEGKLFIRKICPE
HGEFEDLYYGDSSLYYKFDYWEYEGKGPKVPYVDLKSPCPYNCGLCPMHHQHSALVNLVITNRCDLSCWYCFFYAEKAGY
VYEPTLEQIKFMVDQLKKQDITLVIQVTGGEPTLREDIIEIVKMLRENGVKHIQLNSWGGTFAKLYLEDPDKAIKYARAL
REAGVNTVYMSFDGTTRKTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTVNDNDLGNIVKFAAYNMDVVRSVNFQPVS
LTGMMKRNMRAKFRITIPEVIKNIEEQTDGEITKDSWYPIGTSVVFSRLVEALTGKEQFEMANHPSCGAGTYVYVEWRNH
EPHFIPISKFIDLEGLLEYLKEKEEELREGANKVWVASKVLYSIRKFIDKEQGPKDFDVYKMLYNIIIHHNYEALGEWHY
RTLFLGTMHFMDLYNYDVQRVMRCDIHYVVPDGRVIPFCTYNVLNDLYRDKVLREYQIPLDKWTKEHGENSLGDAIKYKR
MASTLENSEIYKNTYKSFQQM

Specific function: Unknown

COG id: COG1964

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 67646; Mature: 67514

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTVNTERHSRLLSAPSSFEEGVVKFGDKEIKIGGPLPTLAKDEKLVRVTHSLCPACYR
CCCCCCCHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LLPATIFEKEGKLFIRKICPEHGEFEDLYYGDSSLYYKFDYWEYEGKGPKVPYVDLKSPC
HHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCEEEEEEEEEEECCCCCCCCEEECCCCC
PYNCGLCPMHHQHSALVNLVITNRCDLSCWYCFFYAEKAGYVYEPTLEQIKFMVDQLKKQ
CCCCCCCCCCCCCHHEEEEEEECCCCCEEEEEEEEECCCCCEECCHHHHHHHHHHHHCCC
DITLVIQVTGGEPTLREDIIEIVKMLRENGVKHIQLNSWGGTFAKLYLEDPDKAIKYARA
CEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHHHHHHHH
LREAGVNTVYMSFDGTTRKTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTVNDNDLGN
HHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHCCCCCHHH
IVKFAAYNMDVVRSVNFQPVSLTGMMKRNMRAKFRITIPEVIKNIEEQTDGEITKDSWYP
HHHHHHCCHHHHHCCCCCEEEHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEECCCCCC
IGTSVVFSRLVEALTGKEQFEMANHPSCGAGTYVYVEWRNHEPHFIPISKFIDLEGLLEY
CCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHHHHH
LKEKEEELREGANKVWVASKVLYSIRKFIDKEQGPKDFDVYKMLYNIIIHHNYEALGEWH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCHH
YRTLFLGTMHFMDLYNYDVQRVMRCDIHYVVPDGRVIPFCTYNVLNDLYRDKVLREYQIP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
LDKWTKEHGENSLGDAIKYKRMASTLENSEIYKNTYKSFQQM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQTVNTERHSRLLSAPSSFEEGVVKFGDKEIKIGGPLPTLAKDEKLVRVTHSLCPACYR
CCCCCCHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
LLPATIFEKEGKLFIRKICPEHGEFEDLYYGDSSLYYKFDYWEYEGKGPKVPYVDLKSPC
HHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCEEEEEEEEEEECCCCCCCCEEECCCCC
PYNCGLCPMHHQHSALVNLVITNRCDLSCWYCFFYAEKAGYVYEPTLEQIKFMVDQLKKQ
CCCCCCCCCCCCCHHEEEEEEECCCCCEEEEEEEEECCCCCEECCHHHHHHHHHHHHCCC
DITLVIQVTGGEPTLREDIIEIVKMLRENGVKHIQLNSWGGTFAKLYLEDPDKAIKYARA
CEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHHHHHHHH
LREAGVNTVYMSFDGTTRKTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTVNDNDLGN
HHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHCCCCCHHH
IVKFAAYNMDVVRSVNFQPVSLTGMMKRNMRAKFRITIPEVIKNIEEQTDGEITKDSWYP
HHHHHHCCHHHHHCCCCCEEEHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEECCCCCC
IGTSVVFSRLVEALTGKEQFEMANHPSCGAGTYVYVEWRNHEPHFIPISKFIDLEGLLEY
CCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHHHHH
LKEKEEELREGANKVWVASKVLYSIRKFIDKEQGPKDFDVYKMLYNIIIHHNYEALGEWH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCHH
YRTLFLGTMHFMDLYNYDVQRVMRCDIHYVVPDGRVIPFCTYNVLNDLYRDKVLREYQIP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
LDKWTKEHGENSLGDAIKYKRMASTLENSEIYKNTYKSFQQM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]