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The map label for this gene is thiI [H]

Identifier: 227828032

GI number: 227828032

Start: 1639798

End: 1640919

Strand: Direct

Name: thiI [H]

Synonym: M1425_1769

Alternate gene names: 227828032

Gene position: 1639798-1640919 (Clockwise)

Preceding gene: 227828031

Following gene: 227828045

Centisome position: 62.86

GC content: 34.4

Gene sequence:

>1122_bases
ATGCTAATCATCATAAGACCATCGGGTGAAATTGCGCTAAAATCTCCCAGATCCAGAAGAAATTTTGAGCACACGCTAGC
CAATAATATAAGAAGTGTTATAAAAGAAGGGAAAATATGGAGATCGCAAGGCGTATTATTTTTAGAAGTTAACGATGACA
ATAAGAACATAGAGGAGCTTAGTAAGGTATTTGGAATAGCCTCCTTTTCACCAGTTATGTCGATTAAATCTTACAATAAT
AATTTAGAAGATATTATTAACAAAGCTAAGGAAGTCTTTGCTGAAATAGTCAAAGGTAAGATTTTTTCTGTAAGAGCTAA
AAGAATAGGTTCTCATAATTTTACCAGTCTAGATGTACAAAGGAAAGTTGGTGAAGCTTTATATCCATTTTCCAGAGGAG
TTAATCTAGAGAATCCTGAAGTTGAGGTATTCATAGAAATTAGGAATGATGTAGCGTATTTTTATCATAAAATTATTAAG
GGCCCTAAGGGGTTGCCTGTTGGAGTAGCTGGAAAGACCGTTGTGTTGTTTTCCGGAGGAATTGATTCGCCCGTAGCCAC
ATGGATGATGATGAAAAGAGGTTCAATTCCCGTTATTTTAAACTTCAATTTAGGAGGAAGTGTACATAGGAAGTTTGTAT
TGGAAGAATTAAGTGTCCTTAGAAAATGGAGCGGAGGACATAAATTAAAACTATTTATCGTAAATGGCACAGACGTGTTA
ATTAAATTATCACAGATTGAGAAAAGAAATCGTGTGGTCATGTTAAAGCGAGTAATGTATAAGGTAGCTGAAAGGTTATG
TGATAAGGCAAACGCTAAATCTATAACCACAGGGGAATCTCTGTCACAAGTCTCATCACAAACCATGACTAATTTATACG
TAACTGAATACGGGATAAAATATCCAATTTTTAGACCTTTAATTGGATTTGATAAAGAAGAGATTGTCGAACTTGCAAGG
AAAATTGGAACTTATGAATATTCAATAAAACTCCCAGAATATTGTGCAATATCAACTAAAGCTAGGACGTCTGTAGAACT
TGATGAAGTACTAAAGGATGAGGAGAATCTAAACATTGATTACGAGAAAGTACTTGAAAACTCAGAGGTAATTGAAATAT
GA

Upstream 100 bases:

>100_bases
ATTAACGAGTTTAAAATTGTTGATTCTGAGTGTGAATGTGGACAAAAATTTTGTAGACATACGGTACAGCTATATTTACA
CTACATGAGAGTGAAGAATA

Downstream 100 bases:

>100_bases
TCAGCCCTCACACGCGTACATCATTTATCAGTCCGGGGTTCTCACATCACCTAACCATAAATGACGAAGCTTGTTTCAGT
CTTTTACCATTAAAAAAGAG

Product: thiamine biosynthesis/tRNA modification protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLIIIRPSGEIALKSPRSRRNFEHTLANNIRSVIKEGKIWRSQGVLFLEVNDDNKNIEELSKVFGIASFSPVMSIKSYNN
NLEDIINKAKEVFAEIVKGKIFSVRAKRIGSHNFTSLDVQRKVGEALYPFSRGVNLENPEVEVFIEIRNDVAYFYHKIIK
GPKGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSIPVILNFNLGGSVHRKFVLEELSVLRKWSGGHKLKLFIVNGTDVL
IKLSQIEKRNRVVMLKRVMYKVAERLCDKANAKSITTGESLSQVSSQTMTNLYVTEYGIKYPIFRPLIGFDKEEIVELAR
KIGTYEYSIKLPEYCAISTKARTSVELDEVLKDEENLNIDYEKVLENSEVIEI

Sequences:

>Translated_373_residues
MLIIIRPSGEIALKSPRSRRNFEHTLANNIRSVIKEGKIWRSQGVLFLEVNDDNKNIEELSKVFGIASFSPVMSIKSYNN
NLEDIINKAKEVFAEIVKGKIFSVRAKRIGSHNFTSLDVQRKVGEALYPFSRGVNLENPEVEVFIEIRNDVAYFYHKIIK
GPKGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSIPVILNFNLGGSVHRKFVLEELSVLRKWSGGHKLKLFIVNGTDVL
IKLSQIEKRNRVVMLKRVMYKVAERLCDKANAKSITTGESLSQVSSQTMTNLYVTEYGIKYPIFRPLIGFDKEEIVELAR
KIGTYEYSIKLPEYCAISTKARTSVELDEVLKDEENLNIDYEKVLENSEVIEI
>Mature_373_residues
MLIIIRPSGEIALKSPRSRRNFEHTLANNIRSVIKEGKIWRSQGVLFLEVNDDNKNIEELSKVFGIASFSPVMSIKSYNN
NLEDIINKAKEVFAEIVKGKIFSVRAKRIGSHNFTSLDVQRKVGEALYPFSRGVNLENPEVEVFIEIRNDVAYFYHKIIK
GPKGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSIPVILNFNLGGSVHRKFVLEELSVLRKWSGGHKLKLFIVNGTDVL
IKLSQIEKRNRVVMLKRVMYKVAERLCDKANAKSITTGESLSQVSSQTMTNLYVTEYGIKYPIFRPLIGFDKEEIVELAR
KIGTYEYSIKLPEYCAISTKARTSVELDEVLKDEENLNIDYEKVLENSEVIEI

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=387, Percent_Identity=32.8165374677003, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 42239; Mature: 42239

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIIRPSGEIALKSPRSRRNFEHTLANNIRSVIKEGKIWRSQGVLFLEVNDDNKNIEEL
CEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHCCEEEEEECCCCCCHHHH
SKVFGIASFSPVMSIKSYNNNLEDIINKAKEVFAEIVKGKIFSVRAKRIGSHNFTSLDVQ
HHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCEEHHH
RKVGEALYPFSRGVNLENPEVEVFIEIRNDVAYFYHKIIKGPKGLPVGVAGKTVVLFSGG
HHHHHHHCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECC
IDSPVATWMMMKRGSIPVILNFNLGGSVHRKFVLEELSVLRKWSGGHKLKLFIVNGTDVL
CCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEE
IKLSQIEKRNRVVMLKRVMYKVAERLCDKANAKSITTGESLSQVSSQTMTNLYVTEYGIK
EEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCC
YPIFRPLIGFDKEEIVELARKIGTYEYSIKLPEYCAISTKARTSVELDEVLKDEENLNID
CCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCHHHHHCCCCCCCCC
YEKVLENSEVIEI
HHHHHCCCCEECC
>Mature Secondary Structure
MLIIIRPSGEIALKSPRSRRNFEHTLANNIRSVIKEGKIWRSQGVLFLEVNDDNKNIEEL
CEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHCCEEEEEECCCCCCHHHH
SKVFGIASFSPVMSIKSYNNNLEDIINKAKEVFAEIVKGKIFSVRAKRIGSHNFTSLDVQ
HHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCEEHHH
RKVGEALYPFSRGVNLENPEVEVFIEIRNDVAYFYHKIIKGPKGLPVGVAGKTVVLFSGG
HHHHHHHCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECC
IDSPVATWMMMKRGSIPVILNFNLGGSVHRKFVLEELSVLRKWSGGHKLKLFIVNGTDVL
CCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEE
IKLSQIEKRNRVVMLKRVMYKVAERLCDKANAKSITTGESLSQVSSQTMTNLYVTEYGIK
EEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCC
YPIFRPLIGFDKEEIVELARKIGTYEYSIKLPEYCAISTKARTSVELDEVLKDEENLNID
CCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCHHHHHCCCCCCCCC
YEKVLENSEVIEI
HHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA