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The map label for this gene is truB [H]

Identifier: 227827978

GI number: 227827978

Start: 1584055

End: 1584303

Strand: Direct

Name: truB [H]

Synonym: M1425_1711

Alternate gene names: 227827978

Gene position: 1584055-1584303 (Clockwise)

Preceding gene: 227827977

Following gene: 227827979

Centisome position: 60.72

GC content: 33.33

Gene sequence:

>1043_bases
ATGATTCTAAATGATTTTATTTACAAAATAGATAATTTTTGTGGATATAAAAATGAATGGAAGATAAGAAAAGATAGTGA
GACATCAGATACATACGGATATTATCCAGAGAAAAGACCAATAGACGTACATATAAAAAACTCAATAATAAACCTTGATA
AACCTCCCGGACCAACAAGTCATGAAGTGGCGTATTGGATTAAAAAGATGTTAAACGTTAACAAAGCGGGACATGGGGGG
ACCCTGGAGCCCATTTCACTGGGCGGGGTAATCCAAAAGTTACCGGCGTATTACCGATAGGAATAGAGAACGCAACTAAA
ATAATGAATTACATAAGTAAAGGAGGAAAAGAGTACGTATGCGTAATGCAAGTCCACTGTGAATACGATAAAGAGGAATT
GACAAAGATAATAAAATCGTTTGAAGGAGAAATATACCAAAGACCACCAGTTAGATCATCAGTCAAGAGGAGATTGAGAG
TTAGAAGTATATATGATATAGAAATACTTGACATGGACAAGAAATTAGTACTATTCAGAGTAAGTTGTGACTCTGGAACT
TACATGAGAAAATTATGCCATGACATAGGGATAATTTACGGTTGTGGGGCACACATGAGAGAGTTAAGAAGAACTAGATC
AGGGATTTTCACAGAATCTACAAATTTAGTGAAGCTACATGACCTTTCTGAAGCAATATACTTATACAAGAACTGCAAGG
ACGAAACGGAATTGAGAAGAGTACTTATGCCAATGGAATATGCGACATGTGAAATGCCCAAACTTATAATAGAGGACTCA
GCAGTAAATGCGTTAGCTTACGGTGCACAACTAGCAGTACCCGGTATAGTAGCCTACCAGAACTTCAAAAGAAACGATAC
AGTTGCCGTATTAACACTTAAGGGGGAATTAGTAGCGACGGGTAACGCATTAATCGATAGTAAAGAGTTAGAAAACGCCA
AAAAGGGTATAGTGGTCAATTTGAGTAGAGTTTTTATGCAGAGAGATATATATCCGAAAGCATGGAAAAAACATGAGTCA
TGA

Upstream 100 bases:

>100_bases
GAGCCTCAGATGAGGAAGTAAGGAAGAAAATAGAGGAAGCTGGTTTAACAGATTACATGAAAGAGAGAATAAAGATTAAA
ATACCAACATTGTGATTTAG

Downstream 100 bases:

>100_bases
ATACGCGATTTTTGAAGTAATTAATGGTGTACCTCTAACCCTTCTCTCCTCCCCGGGGATATTTTCAAAAAAGGAATTAG
ACTTGGGGACAAGAGTACTT

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 83; Mature: 83

Protein sequence:

>339_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDTYGYYPEKRPIDVHIKNSIINLDKPPGPTSHEVAYWIKKMLNVNKAGHGG
TLEPKVTGVLPIGIENATKIMNYISKGGKEYVCVMQVHCEYDKEELTKIIKSFEGEIYQRPPVRSSVKRRLRVRSIYDIE
ILDMDKKLVLFRVSCDSGTYMRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHDLSEAIYLYKNCKDETELRRV
LMPMEYATCEMPKLIIEDSAVNALAYGAQLAVPGIVAYQNFKRNDTVAVLTLKGELVATGNALIDSKELENAKKGIVVNL
SRVFMQRDIYPKAWKKHES

Sequences:

>Translated_83_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDTYGYYPEKRPIDVHIKNSIINLDKPPGPTSHEVAYWIKKMLNVNKAGHGG
TLE
>Mature_83_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDTYGYYPEKRPIDVHIKNSIINLDKPPGPTSHEVAYWIKKMLNVNKAGHGG
TLE

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323204, Length=62, Percent_Identity=45.1612903225806, Blast_Score=62, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281364189, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364187, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364185, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364183, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62471759, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17975520, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 9661; Mature: 9661

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNDFIYKIDNFCGYKNEWKIRKDSETSDTYGYYPEKRPIDVHIKNSIINLDKPPGPTS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCC
HEVAYWIKKMLNVNKAGHGGTLE
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MILNDFIYKIDNFCGYKNEWKIRKDSETSDTYGYYPEKRPIDVHIKNSIINLDKPPGPTS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCC
HEVAYWIKKMLNVNKAGHGGTLE
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]