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The map label for this gene is glyS [H]

Identifier: 227827929

GI number: 227827929

Start: 1543589

End: 1545313

Strand: Direct

Name: glyS [H]

Synonym: M1425_1662

Alternate gene names: 227827929

Gene position: 1543589-1545313 (Clockwise)

Preceding gene: 227827927

Following gene: 227827930

Centisome position: 59.17

GC content: 35.83

Gene sequence:

>1725_bases
GTGAGTGAAGTAATAGATAAAGTGTTGGACTTAGCTAAAAGAAGAGGAATATTTTGGTCATCGTATGAAATTTATGGAGG
AATAGCGGGATTTTACGATATAGGACCTATAGGTGTAAAGATTAAAAATAGAATAGCAGAGTTATGGCGAAAATATTTCG
TGAAGGATAACAGTGACTTTGTAGTAGAAATAGAAAGTCCAATGATAGGCCCTGCTAAAGTATTTGAAGCCAGTGGTCAT
GTGGAAAGTTTTACAGACCCTATCGTTGAGTGTAATAATTGTAAAAGAATATATAGGGCTGATCATTTAATCGAAGACGT
ATTAAAGAGAAGCGTAGAGGGTCTTAAGCCAGAGGAACTAACGAGAATAATAAGGGAAAACAATATTAGATGTCAATATT
GTGGGGGAGAGTTAGGAGAAGTAAGATTATTCAATTTACTTTTCACAACCAACATAGGTCCTTATACTGGTAATTTAGGT
TACATTAGACCAGAGACAGCTCAAGGAATGTTTACTGCATTTAAACGTGTATATGAGGCTACTAGACAAAGACTACCTAT
TGGAATAGCTCAAGTAGGGAGAGTGGGTAGAAATGAGATCTCACCTAGACAAGGGTTAATAAGAATGAGGGAGTTCACCA
TAATGGAGGTAGAGTTTTTCATAGACCCAGAGGATAATAATATTGAAACCATACCATTACATAGATTTAAGGAGACTAAG
CTCAATATTTTGACTGCGAGTGAAAAGGAAAAAAATGGTAAACCACAAGAGTTTAATTTAGAAGAGAGTGTAAGAGAGAA
GATTATTATCCAACCTTGGATGGCATATTGGATGGCTGTAGCATCGAGTTTTGTCAAAGCGCTAGGCATTAACGAATTTT
ATTTTGAAGAGAAGTTACCTTACGAAAGAGCACATTATTCTAAACAGACTTTCGACCAAATTGCTGTAATTAATGGTATG
AAGGTTGAGATTTCTGGGCACGCATATAGAGGAGATTACGATTTATCAAGGCACATGAAATATAGCGGACAAGATTTGAC
TATTTTTAGAAAATATAAAGAACCTAAAACGATTAAAAAGAAGAATGTCATAGTAAACAATACGAAATTAAAGGATAAGG
AACTAAGAAAAAAGGTTATGGAGCTATTAAATGGGAAATCCGCTGAGGAAATTGAGCAAATGATTAGGAACAATGTGACG
ATAGATGGCAAACCTTTAGGTGAATTCATAAGCATTGTTGAGAGAGAAGAGAAGGTTCACGGTGAGCACTTTATACCACA
TGTTGTAGAGCCATCATTTGGCGTTGAAAGGACGTTATTCCTAACTATACTGAGCGCATATAGGGAAAAAGAGGGTAGAG
TTTTGCTATCCTTGCCTAGACATCTAGCGCCTTACGATATTGCAGTTTTCCCACTTCTGGAAAGGGAAGAACTAATTAGG
AAGTCAATGGAAATACGAGAAAATCTTTCCGAAAAATACGAAGTACTATACGATGATTCTGGAAGCATTGGAAGAAGGTA
TGCTAGAGCTGATGAGATTGGCATACCTTTTGCTATTACTGTTGATCCTCAGACATTACTAGATAACAGCGTAACAATAA
GGGATAGAGATAGTTGGAAGCAAGTTAGAGTTAAAATAGATGACCTTAAGATTTCATTGGAAAAGTTATTTAGAGGAGAA
GTCTTTAATAAGATTGGAATTGAGGTGAATGATAGTAATGAGTAA

Upstream 100 bases:

>100_bases
AAATTTCCTACTATTCTCATTTAAATAAAGTAATCTTCCGTCGCTCACTCGCTAATTTTGGTCATAGAACTTTATAAACT
TGTTGTAATGTTAGAGAGTT

Downstream 100 bases:

>100_bases
TAATGAGAATGAAAAAGAAGAAGAGAAAAAGGTTGATATAAAAACTCTTGTTAGTGTTATACCTCCAGCCTCAGCATTAA
AGGGGAGTAAAGCTCCACCG

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MSEVIDKVLDLAKRRGIFWSSYEIYGGIAGFYDIGPIGVKIKNRIAELWRKYFVKDNSDFVVEIESPMIGPAKVFEASGH
VESFTDPIVECNNCKRIYRADHLIEDVLKRSVEGLKPEELTRIIRENNIRCQYCGGELGEVRLFNLLFTTNIGPYTGNLG
YIRPETAQGMFTAFKRVYEATRQRLPIGIAQVGRVGRNEISPRQGLIRMREFTIMEVEFFIDPEDNNIETIPLHRFKETK
LNILTASEKEKNGKPQEFNLEESVREKIIIQPWMAYWMAVASSFVKALGINEFYFEEKLPYERAHYSKQTFDQIAVINGM
KVEISGHAYRGDYDLSRHMKYSGQDLTIFRKYKEPKTIKKKNVIVNNTKLKDKELRKKVMELLNGKSAEEIEQMIRNNVT
IDGKPLGEFISIVEREEKVHGEHFIPHVVEPSFGVERTLFLTILSAYREKEGRVLLSLPRHLAPYDIAVFPLLEREELIR
KSMEIRENLSEKYEVLYDDSGSIGRRYARADEIGIPFAITVDPQTLLDNSVTIRDRDSWKQVRVKIDDLKISLEKLFRGE
VFNKIGIEVNDSNE

Sequences:

>Translated_574_residues
MSEVIDKVLDLAKRRGIFWSSYEIYGGIAGFYDIGPIGVKIKNRIAELWRKYFVKDNSDFVVEIESPMIGPAKVFEASGH
VESFTDPIVECNNCKRIYRADHLIEDVLKRSVEGLKPEELTRIIRENNIRCQYCGGELGEVRLFNLLFTTNIGPYTGNLG
YIRPETAQGMFTAFKRVYEATRQRLPIGIAQVGRVGRNEISPRQGLIRMREFTIMEVEFFIDPEDNNIETIPLHRFKETK
LNILTASEKEKNGKPQEFNLEESVREKIIIQPWMAYWMAVASSFVKALGINEFYFEEKLPYERAHYSKQTFDQIAVINGM
KVEISGHAYRGDYDLSRHMKYSGQDLTIFRKYKEPKTIKKKNVIVNNTKLKDKELRKKVMELLNGKSAEEIEQMIRNNVT
IDGKPLGEFISIVEREEKVHGEHFIPHVVEPSFGVERTLFLTILSAYREKEGRVLLSLPRHLAPYDIAVFPLLEREELIR
KSMEIRENLSEKYEVLYDDSGSIGRRYARADEIGIPFAITVDPQTLLDNSVTIRDRDSWKQVRVKIDDLKISLEKLFRGE
VFNKIGIEVNDSNE
>Mature_573_residues
SEVIDKVLDLAKRRGIFWSSYEIYGGIAGFYDIGPIGVKIKNRIAELWRKYFVKDNSDFVVEIESPMIGPAKVFEASGHV
ESFTDPIVECNNCKRIYRADHLIEDVLKRSVEGLKPEELTRIIRENNIRCQYCGGELGEVRLFNLLFTTNIGPYTGNLGY
IRPETAQGMFTAFKRVYEATRQRLPIGIAQVGRVGRNEISPRQGLIRMREFTIMEVEFFIDPEDNNIETIPLHRFKETKL
NILTASEKEKNGKPQEFNLEESVREKIIIQPWMAYWMAVASSFVKALGINEFYFEEKLPYERAHYSKQTFDQIAVINGMK
VEISGHAYRGDYDLSRHMKYSGQDLTIFRKYKEPKTIKKKNVIVNNTKLKDKELRKKVMELLNGKSAEEIEQMIRNNVTI
DGKPLGEFISIVEREEKVHGEHFIPHVVEPSFGVERTLFLTILSAYREKEGRVLLSLPRHLAPYDIAVFPLLEREELIRK
SMEIRENLSEKYEVLYDDSGSIGRRYARADEIGIPFAITVDPQTLLDNSVTIRDRDSWKQVRVKIDDLKISLEKLFRGEV
FNKIGIEVNDSNE

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=606, Percent_Identity=30.8580858085809, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32564541, Length=596, Percent_Identity=33.7248322147651, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17554996, Length=596, Percent_Identity=33.7248322147651, Blast_Score=327, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6325338, Length=599, Percent_Identity=31.7195325542571, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319597, Length=613, Percent_Identity=30.1794453507341, Blast_Score=267, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24664462, Length=580, Percent_Identity=35.6896551724138, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21357965, Length=580, Percent_Identity=35.6896551724138, Blast_Score=329, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 66488; Mature: 66357

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVIDKVLDLAKRRGIFWSSYEIYGGIAGFYDIGPIGVKIKNRIAELWRKYFVKDNSDF
CHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VVEIESPMIGPAKVFEASGHVESFTDPIVECNNCKRIYRADHLIEDVLKRSVEGLKPEEL
EEEECCCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TRIIRENNIRCQYCGGELGEVRLFNLLFTTNIGPYTGNLGYIRPETAQGMFTAFKRVYEA
HHHHHHCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TRQRLPIGIAQVGRVGRNEISPRQGLIRMREFTIMEVEFFIDPEDNNIETIPLHRFKETK
HHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCCEEEECCCHHCCCE
LNILTASEKEKNGKPQEFNLEESVREKIIIQPWMAYWMAVASSFVKALGINEFYFEEKLP
EEEEECCCHHCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
YERAHYSKQTFDQIAVINGMKVEISGHAYRGDYDLSRHMKYSGQDLTIFRKYKEPKTIKK
HHHHHCCHHHHHHHHHCCCEEEEECCCEECCCCCHHHHCCCCCCCEEEEEECCCCCCHHH
KNVIVNNTKLKDKELRKKVMELLNGKSAEEIEQMIRNNVTIDGKPLGEFISIVEREEKVH
CCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHC
GEHFIPHVVEPSFGVERTLFLTILSAYREKEGRVLLSLPRHLAPYDIAVFPLLEREELIR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCHHHHHHHH
KSMEIRENLSEKYEVLYDDSGSIGRRYARADEIGIPFAITVDPQTLLDNSVTIRDRDSWK
HHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCEEEECCCCCE
QVRVKIDDLKISLEKLFRGEVFNKIGIEVNDSNE
EEEEEEEHHHHHHHHHHHHHHHHHHCCEECCCCC
>Mature Secondary Structure 
SEVIDKVLDLAKRRGIFWSSYEIYGGIAGFYDIGPIGVKIKNRIAELWRKYFVKDNSDF
HHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VVEIESPMIGPAKVFEASGHVESFTDPIVECNNCKRIYRADHLIEDVLKRSVEGLKPEEL
EEEECCCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TRIIRENNIRCQYCGGELGEVRLFNLLFTTNIGPYTGNLGYIRPETAQGMFTAFKRVYEA
HHHHHHCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TRQRLPIGIAQVGRVGRNEISPRQGLIRMREFTIMEVEFFIDPEDNNIETIPLHRFKETK
HHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCCEEEECCCHHCCCE
LNILTASEKEKNGKPQEFNLEESVREKIIIQPWMAYWMAVASSFVKALGINEFYFEEKLP
EEEEECCCHHCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
YERAHYSKQTFDQIAVINGMKVEISGHAYRGDYDLSRHMKYSGQDLTIFRKYKEPKTIKK
HHHHHCCHHHHHHHHHCCCEEEEECCCEECCCCCHHHHCCCCCCCEEEEEECCCCCCHHH
KNVIVNNTKLKDKELRKKVMELLNGKSAEEIEQMIRNNVTIDGKPLGEFISIVEREEKVH
CCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHC
GEHFIPHVVEPSFGVERTLFLTILSAYREKEGRVLLSLPRHLAPYDIAVFPLLEREELIR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCHHHHHHHH
KSMEIRENLSEKYEVLYDDSGSIGRRYARADEIGIPFAITVDPQTLLDNSVTIRDRDSWK
HHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCEEEECCCCCE
QVRVKIDDLKISLEKLFRGEVFNKIGIEVNDSNE
EEEEEEEHHHHHHHHHHHHHHHHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]