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The map label for this gene is tfb [H]

Identifier: 227827925

GI number: 227827925

Start: 1541193

End: 1542122

Strand: Direct

Name: tfb [H]

Synonym: M1425_1658

Alternate gene names: 227827925

Gene position: 1541193-1542122 (Clockwise)

Preceding gene: 227827922

Following gene: 227827927

Centisome position: 59.08

GC content: 39.03

Gene sequence:

>930_bases
GTGTTGTATTTGTCTGAAGAAAATAAACCTGTATCTACTCCTTGCCCTCCCGATAAAATTATATTCGATGCGGAGAGGGG
GGAATATATTTGCTCTGAAACTGGAGAAGTTTTAGAAGATAAAATTATAGATCAAGGACCAGAATGGAGGGCATTTACTC
CAGAAGAAAAAGAAAAGAGAAGCAGAGTTGGAGGGCCTTTAAATAATACTATTCACGATAGGGGCTTATCCACTCTTATA
GACTGGAAAGATAAGGACGCAATGGGAAGAACCTTAGACCCTAAGAGAAGACTTGAAGCATTAAGATGGAGAAAATGGCA
GATAAGGGCTAGAATACAAAGTTCTATAGATAGGAACTTAGCACAAGCTATGAATGAATTAGAAAGAATTGGGAACTTGC
TGAACTTGCCAAAATCAGTTAAGGATGAGGCTGCACTAATCTACAGAAAAGCAGTAGAGAAAGGGTTAGTAAGAGGAAGG
AGTATAGAAAGCGTTGTAGCAGCTGCAATATACGCAGCATGTAGAAGGATGAAGTTAGCTAGAACTTTGGATGAGATAGC
ACAATATACTAAGGCTAATAGGAAGGAAGTAGCTAGATGCTATAGACTATTACTTAGAGAGTTAGATGTCAGTGTGCCAG
TAAGCGATCCAAAGGATTACGTAACTAGAATAGCTAACTTGTTAGGCCTAAGTGGAGCCGTTATGAAAACAGCTGCCGAA
ATTATAGATAAGGCGAAGGGTTCTGGTTTAACTGCTGGTAAGGATCCAGCTGGCTTAGCCGCAGCTGCGATTTATATAGC
GTCATTATTACACGATGAAAGAAGAACACAAAAAGAAATAGCGCAAGTTGCAGGCGTTACTGAAGTTACTGTGAGAAATA
GGTATAAAGAACTGACACAAGAACTAAAAATTTCAATACCTACTCAATAA

Upstream 100 bases:

>100_bases
CCATTTTTAGATAAGTAAGGAGAGACATGGGGGAAAGATTTATAAGGAAAGAAATAGAAAAGTTTAAAAGGTTTACTATT
CGTGGCTGTATATAATTGGG

Downstream 100 bases:

>100_bases
CTAGAATGTTAGATTCCGCATATCCCATTTTTATTAAGATTTCCCTAACTTTTTCTTTATGATCTCCTTGAATTAGTATT
TTACCATCTTTTACTGTACC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 1 [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLYLSEENKPVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ

Sequences:

>Translated_309_residues
MLYLSEENKPVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ
>Mature_309_residues
MLYLSEENKPVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLI
DWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGR
SIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TFIIB family [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=288, Percent_Identity=35.0694444444444, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI22035556, Length=291, Percent_Identity=28.8659793814433, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17564892, Length=293, Percent_Identity=32.0819112627986, Blast_Score=163, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17531397, Length=289, Percent_Identity=24.5674740484429, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6325343, Length=309, Percent_Identity=28.4789644012945, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321685, Length=193, Percent_Identity=26.9430051813472, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136758, Length=292, Percent_Identity=35.958904109589, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI281361961, Length=289, Percent_Identity=25.2595155709343, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24647658, Length=289, Percent_Identity=25.2595155709343, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 34681; Mature: 34681

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLSEENKPVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKR
CEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
SRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNL
HHCCCCCCCHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ELKISIPTQ
HHHHCCCCC
>Mature Secondary Structure
MLYLSEENKPVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKR
CEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
SRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNL
HHCCCCCCCHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ELKISIPTQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]