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The map label for this gene is pgk [H]

Identifier: 227827847

GI number: 227827847

Start: 1470028

End: 1471275

Strand: Direct

Name: pgk [H]

Synonym: M1425_1580

Alternate gene names: 227827847

Gene position: 1470028-1471275 (Clockwise)

Preceding gene: 227827846

Following gene: 227827848

Centisome position: 56.35

GC content: 37.02

Gene sequence:

>1248_bases
GTGGGGATCTTTCTGATCAAAGTGGGAGATCTAACTATACCCACAATGGACGATATAGATATAAGATCTAAGAAAGTTCT
ATTACGTATTGATATAAATTCGCCTGTCGATGAAAACGGCAAAATAATCGATGACTCTAGAATAAAGGCACATATTGGGA
CTATAAAGGAACTAGCAGATAAGGGTAACTCTGTCGTGCTAATTTCACATCAAGGTAGGCCTGGGGATAAGGATTTCATA
AGTCTAGAGGAGCATGCTAAATTAATCTCAAAGTATTTAGATCGAGAAGTAGTTTTCGTAGAAGACGTAATAGGCCCATA
TGCTAGAGAAATGATCAAAAAACTAGAAAATAATGGAATTTTGCTTTTAGATAATATAAGACTAATCTCAGAGGAGTTAA
TAGAGGCTCCGCCACAACAACATGTAAAAACCTTTTTAGTAAGGAAATTAGCCCCACTTTTTGACGTTTATGTCAATGAC
GCATTTGCTACTTCACATAGAAGTCAGCCGAGTATTGTAGGCTTTCCTCTAGCTTTACCATCAGCTGCAGGAAGAGTAAT
GGAAAGGGAAGTTTCAGCTTTAGCTAGAATTTTTAATGCTGAAGATACACCAAAAATATTTACATTAGGTGGCGGGAAGG
TTCACGACACTATTAGAATAATTGAAAATCTAGTGAGAAAAAGGATAGCAGATAGAATACTTACTGGTGGTCTCGTTGCA
GAACTGTTTTCTGTTGCTAAGGGAATAAATCTAAATCCAAAGAATATGGAAATTTTGGAAAAATTAGGTATTTTGAGCTT
AATTCCTAGAGCTAGAAAACTACTTCTAAGCGGGGCTCCCATCGAAATACCAGTTGATTACAAGGTAGAAATAAACGGAA
ACGTAATGGAGGAACCAGCTAGCAAGATTACTGGTGTGATAAAGGATATCGGATCTACTACTGCGGAAATATACTCATCA
TTTATAAAAGATGCTAAAGTAGTAGTTTTAAGGGGACCAATGGGAGTAATCGAGGATGAAAGATTTAAGAGTGGAAGTAA
AAGCGTCTTGAAGGCGTCATTGGAGGGTCAAGGTTATGTTATAATAGGAGGTGGACATATGATAAGTGCTTTAGATAAGG
ATATGAAGATCGATAGTAGTAAAGTTCATATATCTACTGGTGGAGGAGCTCTTCTCTTATTCTTATCTGGAGAACGGTTA
CCCGCATTAGAAGCATTATCAATGTCGGTGGTGAATAGTAGTGATTAG

Upstream 100 bases:

>100_bases
ATCAGTTAAAGACTAAGACATTAATACCTCTAATTTGGGATCGTTTTATTATCGATTCTCTGCGTTGCTAAAAGTTATTA
CCCTAAAATTCAGTATATAA

Downstream 100 bases:

>100_bases
TGTAGCTGTTAATGGATATGGTACTATAGGTAAGAGAGTCGCTGATGCTATCCTTAAACAACCAGATATGAGACTAATAG
GGGTTGCAAAGACATCTCCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGIFLIKVGDLTIPTMDDIDIRSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELADKGNSVVLISHQGRPGDKDFI
SLEEHAKLISKYLDREVVFVEDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKTFLVRKLAPLFDVYVND
AFATSHRSQPSIVGFPLALPSAAGRVMEREVSALARIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
ELFSVAKGINLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVMEEPASKITGVIKDIGSTTAEIYSS
FIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDKDMKIDSSKVHISTGGGALLLFLSGERL
PALEALSMSVVNSSD

Sequences:

>Translated_415_residues
MGIFLIKVGDLTIPTMDDIDIRSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELADKGNSVVLISHQGRPGDKDFI
SLEEHAKLISKYLDREVVFVEDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKTFLVRKLAPLFDVYVND
AFATSHRSQPSIVGFPLALPSAAGRVMEREVSALARIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
ELFSVAKGINLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVMEEPASKITGVIKDIGSTTAEIYSS
FIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDKDMKIDSSKVHISTGGGALLLFLSGERL
PALEALSMSVVNSSD
>Mature_414_residues
GIFLIKVGDLTIPTMDDIDIRSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELADKGNSVVLISHQGRPGDKDFIS
LEEHAKLISKYLDREVVFVEDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKTFLVRKLAPLFDVYVNDA
FATSHRSQPSIVGFPLALPSAAGRVMEREVSALARIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVAE
LFSVAKGINLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVMEEPASKITGVIKDIGSTTAEIYSSF
IKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDKDMKIDSSKVHISTGGGALLLFLSGERLP
ALEALSMSVVNSSD

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=426, Percent_Identity=33.8028169014084, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI31543397, Length=429, Percent_Identity=32.4009324009324, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1789294, Length=408, Percent_Identity=29.656862745098, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=32.3741007194245, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6319857, Length=418, Percent_Identity=33.2535885167464, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17136394, Length=424, Percent_Identity=32.311320754717, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24581203, Length=419, Percent_Identity=30.5489260143198, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 45253; Mature: 45122

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFLIKVGDLTIPTMDDIDIRSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELAD
CCEEEEEECCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHC
KGNSVVLISHQGRPGDKDFISLEEHAKLISKYLDREVVFVEDVIGPYAREMIKKLENNGI
CCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHCCCCE
LLLDNIRLISEELIEAPPQQHVKTFLVRKLAPLFDVYVNDAFATSHRSQPSIVGFPLALP
EEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECEECC
SAAGRVMEREVSALARIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ELFSVAKGINLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVMEEPA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCCHHHHH
SKITGVIKDIGSTTAEIYSSFIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYV
HHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCEEE
IIGGGHMISALDKDMKIDSSKVHISTGGGALLLFLSGERLPALEALSMSVVNSSD
EEECCCEEHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIFLIKVGDLTIPTMDDIDIRSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELAD
CEEEEEECCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHC
KGNSVVLISHQGRPGDKDFISLEEHAKLISKYLDREVVFVEDVIGPYAREMIKKLENNGI
CCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHCCCCE
LLLDNIRLISEELIEAPPQQHVKTFLVRKLAPLFDVYVNDAFATSHRSQPSIVGFPLALP
EEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECEECC
SAAGRVMEREVSALARIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ELFSVAKGINLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVMEEPA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCCHHHHH
SKITGVIKDIGSTTAEIYSSFIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYV
HHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCEEE
IIGGGHMISALDKDMKIDSSKVHISTGGGALLLFLSGERLPALEALSMSVVNSSD
EEECCCEEHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8521845; 10701121; 11427726 [H]