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The map label for this gene is priB [H]

Identifier: 227827838

GI number: 227827838

Start: 1461440

End: 1462363

Strand: Direct

Name: priB [H]

Synonym: M1425_1571

Alternate gene names: 227827838

Gene position: 1461440-1462363 (Clockwise)

Preceding gene: 227827836

Following gene: 227827840

Centisome position: 56.02

GC content: 30.95

Gene sequence:

>924_bases
ATGGTATTAGACGTTAAGAAGTATCCTTTTATTAAAAGTCTTGAAGACGAACTTAAGAAATATGGAGGGGGAATTACATT
ATCGGATTTACTATTAAATAGCACTACACTCATAGACCAAGCTAAAGATAGAATACAAAAAGTTAAATCTGGTGAGGAAC
TTCCACATTACGTCTCCTATAATGAACCGGTACTCGTTTTTTACACAACATTACTTTCTCTAGCAATATTAAATGATCTT
AGGCTTATTAGAAAATATGCACACATTGAAGCTAAACAATTCAAATCCTTACTTCAAAATGAGAATGAGGAGAATTTACT
TGAAATCACTAAGTTATTAGATCTGAAGATAAATAAATGTGATCAGATAAAATTTTCTTTGGAGAAAAAGAGACGGATAG
TACAAAAGGAGTTTTGTGTTAATTTTATAGACTATCTGAAGTATACAAAAGGTCTTAGGGAGGATTGGAAGCTGAGTAAA
CAAGTCTTATATAAGGGATATGTATATTTGGATAAAAATCAACTAACAGATCTGATAGCTGAAAATATTAAGAATAAAAT
AGTTGAAATGATAAGACCACTGAATCTTAAAGAAATACCAGAAAAATTAAAGAGCTTAATAGAAAAGAAAGGAGTAGTTC
CTCCATGTATAGAAAACATTTTAGGGAAGGAAAAGTTAAGCGAAGAGGAGATAAGGACTTTAATTACATTTTATATTAAC
ATAGGTAAGGGATTAGGTAGCATAATAGCAATTATGAAAAAATGGAACGTTACAAACATCGAGGACTTATACAAGAAATA
TAGAGGAGATAAGGAGACGAGGTATATCGTTTATTCATGTGCTAGGATGAAGCAATTAGGTTTGTGCGTAAGTAATTGTA
ACGTTAAAAATCCCTTGCAGCTCTACTTCCTTAGTAAAGAATAG

Upstream 100 bases:

>100_bases
ATTTACATATGGCCATGCAGATGTAACAAGTACTTTCATTATATTTTGTGTTAAGAGCAAGTCTTTATTAAACATAACTA
AGCATACTATAAGTAAGGAA

Downstream 100 bases:

>100_bases
ACAGCGTGAAACCTCTGGATCACTGTGATCGCAGTGAGTAACATGAATAGATAAAATACATATAAGGAGATCTGTTTACT
AACTATTAGATAAAGTAAGA

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVLDVKKYPFIKSLEDELKKYGGGITLSDLLLNSTTLIDQAKDRIQKVKSGEELPHYVSYNEPVLVFYTTLLSLAILNDL
RLIRKYAHIEAKQFKSLLQNENEENLLEITKLLDLKINKCDQIKFSLEKKRRIVQKEFCVNFIDYLKYTKGLREDWKLSK
QVLYKGYVYLDKNQLTDLIAENIKNKIVEMIRPLNLKEIPEKLKSLIEKKGVVPPCIENILGKEKLSEEEIRTLITFYIN
IGKGLGSIIAIMKKWNVTNIEDLYKKYRGDKETRYIVYSCARMKQLGLCVSNCNVKNPLQLYFLSKE

Sequences:

>Translated_307_residues
MVLDVKKYPFIKSLEDELKKYGGGITLSDLLLNSTTLIDQAKDRIQKVKSGEELPHYVSYNEPVLVFYTTLLSLAILNDL
RLIRKYAHIEAKQFKSLLQNENEENLLEITKLLDLKINKCDQIKFSLEKKRRIVQKEFCVNFIDYLKYTKGLREDWKLSK
QVLYKGYVYLDKNQLTDLIAENIKNKIVEMIRPLNLKEIPEKLKSLIEKKGVVPPCIENILGKEKLSEEEIRTLITFYIN
IGKGLGSIIAIMKKWNVTNIEDLYKKYRGDKETRYIVYSCARMKQLGLCVSNCNVKNPLQLYFLSKE
>Mature_307_residues
MVLDVKKYPFIKSLEDELKKYGGGITLSDLLLNSTTLIDQAKDRIQKVKSGEELPHYVSYNEPVLVFYTTLLSLAILNDL
RLIRKYAHIEAKQFKSLLQNENEENLLEITKLLDLKINKCDQIKFSLEKKRRIVQKEFCVNFIDYLKYTKGLREDWKLSK
QVLYKGYVYLDKNQLTDLIAENIKNKIVEMIRPLNLKEIPEKLKSLIEKKGVVPPCIENILGKEKLSEEEIRTLITFYIN
IGKGLGSIIAIMKKWNVTNIEDLYKKYRGDKETRYIVYSCARMKQLGLCVSNCNVKNPLQLYFLSKE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 35874; Mature: 35874

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLDVKKYPFIKSLEDELKKYGGGITLSDLLLNSTTLIDQAKDRIQKVKSGEELPHYVSY
CEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NEPVLVFYTTLLSLAILNDLRLIRKYAHIEAKQFKSLLQNENEENLLEITKLLDLKINKC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCH
DQIKFSLEKKRRIVQKEFCVNFIDYLKYTKGLREDWKLSKQVLYKGYVYLDKNQLTDLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECHHHHHHHHH
ENIKNKIVEMIRPLNLKEIPEKLKSLIEKKGVVPPCIENILGKEKLSEEEIRTLITFYIN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCHHCCCHHHHHHHHHHHHH
IGKGLGSIIAIMKKWNVTNIEDLYKKYRGDKETRYIVYSCARMKQLGLCVSNCNVKNPLQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LYFLSKE
EEEECCC
>Mature Secondary Structure
MVLDVKKYPFIKSLEDELKKYGGGITLSDLLLNSTTLIDQAKDRIQKVKSGEELPHYVSY
CEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NEPVLVFYTTLLSLAILNDLRLIRKYAHIEAKQFKSLLQNENEENLLEITKLLDLKINKC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCH
DQIKFSLEKKRRIVQKEFCVNFIDYLKYTKGLREDWKLSKQVLYKGYVYLDKNQLTDLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECHHHHHHHHH
ENIKNKIVEMIRPLNLKEIPEKLKSLIEKKGVVPPCIENILGKEKLSEEEIRTLITFYIN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCHHCCCHHHHHHHHHHHHH
IGKGLGSIIAIMKKWNVTNIEDLYKKYRGDKETRYIVYSCARMKQLGLCVSNCNVKNPLQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LYFLSKE
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726 [H]