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The map label for this gene is ilvB [H]

Identifier: 227827820

GI number: 227827820

Start: 1443753

End: 1445471

Strand: Direct

Name: ilvB [H]

Synonym: M1425_1553

Alternate gene names: 227827820

Gene position: 1443753-1445471 (Clockwise)

Preceding gene: 227827819

Following gene: 227827821

Centisome position: 55.34

GC content: 40.31

Gene sequence:

>1719_bases
TTGCCAACAGGTGCACGAATTCTAGTTGACTCTCTAAAAAGAGAAGGAGTTAAGGTAATCTTCGGAATACCTGGACTATC
AAACATGCAGATTTATGATGCTTTCGTGGAAGATTTGGCAAATGGTGAGCTTAGACATGTTCTGATGAGACATGAACAAG
CTGCTGCACATGCTGCAGATGGATACGCAAGAGCTTCTGGAGTCCCTGGAGTGTGTACAGCCACATCTGGTCCAGGTACG
ACCAATTTAACTACAGGACTTATTACTGCATACTGGGATAGTTCTCCCGTAATTGCAATAACCGGTAACGTGCCTAGAAG
CGTTATGGGTAAGATGGCATTCCAAGAAGCTGATGCTATGGGAGTGTTTGAAAATGTAACTAAATACGTTATTGGTATTA
AGAGAATAGATGAAATTCCCCAATGGATTAAGAATGCATTTTACATTGCAACTACTGGAAGACCTGGGCCAGTAGTAATT
GATATTCCTAGGGATATTTTCTATGAAAAAATGGAAGAGATAAAATGGCCAGAGAAACCGCTTGTTAAGGGTTATAGAGA
TTTCCCAACTAGGATAGACCGATTAGCATTGAAAAAGGCTGCTGAGATTCTAATCAATGCAGAGAGACCAATAATTTTAG
TAGGTACTGGGGTAGTATGGGCTAACGCAACCCCAGAAGTTTTAGAGTTAGCAGAACTATTGCATATTCCAATAGTCTCT
ACTTTCCCTGGAAAAACTGCAATACCACATGATCATCCCCTATATTTCGGGCCAATGGGATACTATGGAAGAGCAGAAGC
ATCAATGGCTGCCCTAGAATCTGACGCAATGCTAGTTGTAGGTGCGAGATTTAGCGATAGGACATTTACATCATATGATG
AAATGGTAGAGACTAGAAAGAAATTCATAATGGTAAATATTGATCCGACAGATGGAGAAAAGGCCATAAAGGTAGATGTC
GGAATTTACGGTAATGCCAAGATAATATTAAGAGAGTTAATAAAGGCAATAACGACACTAGGTCAAAAAAGAGATAGAAG
CGCATGGCTGAAAAGAGTTAAAGAGTATAAAGAATACTATTCTCAATTTTACTATACTGAGGAGAACGGAAAATTAAAGC
CTTGGAAGATAATGAAGACCATTAGGCAAGCATTACCAAGGGATGCAATAGTGACCACTGGTGTAGGCCAACATCAGATG
TGGGCTGAAGTGTTTTGGGAAGTATTGGAACCTAGAACTTTTTTAACCTCATCTGGAATGGGTACAATGGGTTTTGGTCT
TCCTGCTGCAATGGGAGCTAAATTAGCTAGATCGGATAAAGTTGTAGTGGATCTAGATGGTGATGGTTCATTCTTAATGA
CCGGAACAAACCTAGCCACAGCTGTAGATGAGCACATTCCGGTAATATCAGTGATATTTGATAATAGAACCTTAGGGTTA
GTAAGACAAGTTCAAGATTTGTTCTTCGGGAAGAGAATAGTAGGTGTAGATTACGGTCCTTCACCAGACTTCGTTAAATT
AGCTGAGGCATTTGGTGCTTTAGGTTTTAACGTAACGACCTATGAGGACATAGAAAAGTCACTTAAGAGTGCGATAAAGG
AAGATATCCCCGCAGTAATCAGAGTACCAGTAGATAAGGAAGAACTGGCCTTACCTACGTTACCACCGGGTGGAAGATTA
AAACAGGTGATCTTACGTGACCCAAGAAAGAGTAGTTAA

Upstream 100 bases:

>100_bases
AAATTTGATACGTATTTCAAAACTTTCAATAGATTTCTTGATAAAGGAAATGAAGATCGAGTTAGATATGAGGTATCTAT
ATACAAGAAGGTGATATAAA

Downstream 100 bases:

>100_bases
GGTATTGGCTTATTATAGGGATCCAGGCTTAATAGAGAGGATAGCATCTAACTTCAGGAAATTATTTATGGACATTGATT
GGATATACGGCTGGAAGGTA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MPTGARILVDSLKREGVKVIFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGT
TNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVI
DIPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDV
GIYGNAKIILRELIKAITTLGQKRDRSAWLKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQALPRDAIVTTGVGQHQM
WAEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARSDKVVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
VRQVQDLFFGKRIVGVDYGPSPDFVKLAEAFGALGFNVTTYEDIEKSLKSAIKEDIPAVIRVPVDKEELALPTLPPGGRL
KQVILRDPRKSS

Sequences:

>Translated_572_residues
MPTGARILVDSLKREGVKVIFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGT
TNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVI
DIPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDV
GIYGNAKIILRELIKAITTLGQKRDRSAWLKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQALPRDAIVTTGVGQHQM
WAEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARSDKVVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
VRQVQDLFFGKRIVGVDYGPSPDFVKLAEAFGALGFNVTTYEDIEKSLKSAIKEDIPAVIRVPVDKEELALPTLPPGGRL
KQVILRDPRKSS
>Mature_571_residues
PTGARILVDSLKREGVKVIFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGTT
NLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVID
IPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVST
FPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDVG
IYGNAKIILRELIKAITTLGQKRDRSAWLKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQALPRDAIVTTGVGQHQMW
AEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARSDKVVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGLV
RQVQDLFFGKRIVGVDYGPSPDFVKLAEAFGALGFNVTTYEDIEKSLKSAIKEDIPAVIRVPVDKEELALPTLPPGGRLK
QVILRDPRKSS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=25.9717314487633, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI21361361, Length=589, Percent_Identity=23.7691001697793, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=582, Percent_Identity=36.426116838488, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790104, Length=555, Percent_Identity=36.2162162162162, Blast_Score=352, Evalue=5e-98,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=31.7604355716878, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=27.8659611992945, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=25.0883392226148, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=555, Percent_Identity=26.3063063063063, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=555, Percent_Identity=26.3063063063063, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=592, Percent_Identity=25.8445945945946, Blast_Score=132, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=35.7876712328767, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320816, Length=566, Percent_Identity=23.6749116607774, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=498, Percent_Identity=24.4979919678715, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19922626, Length=576, Percent_Identity=26.3888888888889, Blast_Score=172, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63203; Mature: 63072

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGARILVDSLKREGVKVIFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAAD
CCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
GYARASGVPGVCTATSGPGTTNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAM
CHHHHCCCCCEEECCCCCCCCCCHHCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
GVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVIDIPRDIFYEKMEEIKWPEKP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHCCCCCCH
LVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCEE
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
KFIMVNIDPTDGEKAIKVDVGIYGNAKIILRELIKAITTLGQKRDRSAWLKRVKEYKEYY
CEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SQFYYTEENGKLKPWKIMKTIRQALPRDAIVTTGVGQHQMWAEVFWEVLEPRTFLTSSGM
HHEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEECCCC
GTMGFGLPAAMGAKLARSDKVVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCEEEEEECCCHHHH
VRQVQDLFFGKRIVGVDYGPSPDFVKLAEAFGALGFNVTTYEDIEKSLKSAIKEDIPAVI
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEE
RVPVDKEELALPTLPPGGRLKQVILRDPRKSS
ECCCCHHHCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTGARILVDSLKREGVKVIFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAAD
CCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
GYARASGVPGVCTATSGPGTTNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAM
CHHHHCCCCCEEECCCCCCCCCCHHCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
GVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVIDIPRDIFYEKMEEIKWPEKP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHCCCCCCH
LVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCEE
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
KFIMVNIDPTDGEKAIKVDVGIYGNAKIILRELIKAITTLGQKRDRSAWLKRVKEYKEYY
CEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SQFYYTEENGKLKPWKIMKTIRQALPRDAIVTTGVGQHQMWAEVFWEVLEPRTFLTSSGM
HHEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEECCCC
GTMGFGLPAAMGAKLARSDKVVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCEEEEEECCCHHHH
VRQVQDLFFGKRIVGVDYGPSPDFVKLAEAFGALGFNVTTYEDIEKSLKSAIKEDIPAVI
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEE
RVPVDKEELALPTLPPGGRLKQVILRDPRKSS
ECCCCHHHCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]