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The map label for this gene is gltB [C]

Identifier: 227827724

GI number: 227827724

Start: 1362598

End: 1364715

Strand: Direct

Name: gltB [C]

Synonym: M1425_1455

Alternate gene names: 227827724

Gene position: 1362598-1364715 (Clockwise)

Preceding gene: 227827723

Following gene: 227827725

Centisome position: 52.23

GC content: 39.19

Gene sequence:

>2118_bases
GTGCCAAAACAATTTAATGATGAATTTTGGACCGTTGAGAAGGTAGAACATATTAGGTACCTCTCTTTGACTGGTAAGCC
TTATAAAATATTCAATGAAGATATGAACTCTTTAAGGGTATTAGATAAAGTTAGATTCAAACTTGATAAGGCAACTTCAA
TTTCTTTAACCCCCAAGGCCAATCTGGAACTAAAATTTTCTGGAATTTACATGAAGTCTCCTTTATATCTAGGTGACATG
TCATACGGAGCGTTAAGTGGTAATCCCAATATAGCAATTGCTACTGCGGCTGATTTGACTGAAACTCTTGCCGGTACGGG
AGAAGGTGGCCTACATCCAGAGGTTGCTAAGCATAAGAGAATTTTTGTGCAGTGGGCGTCAGCTAGATTTGGTGTTGATA
TTAGAGTTTTAACTGCTGGTATGGGTGTTGTCATAAAAATTGGTCAAGGGGCTAAGCCTGGAATTGGCGGCCATTTACCA
GGAAATAAAGTTACTGAACCTATATCTGTCACTAGAAGAATTCCAATTGGTATAGATGCTATATCTCCAGCACCTCATCA
CGATATTTACTCCATCGAGGATCTGGGACAAAGAATAGAGGCATTAAAAGAAGCAACTGGAAAGCCGGTTTTTGTTAAAG
TAGCTGCAACTAATTATATCCCTTACATAGTTTCCGGTATTGCTAGAATGGGGGCAGATGGTGTTATAATAGATGGTCAT
GGAGCGGGAACTGGTGCCACCCCTGTTGTAATAAGAGATAACGTTGGTATACCAATAGAGCTAGCTGTAGCCTCAGCTGA
TAATATTTTAAGGAGAGAAGGACTTAGGGATAAGTTTACTATTATAGCTGCTGGTAGGGTCTCATCAGCAACAGACGCTG
CTAAACTAATTGCTTTAGGCGCTGACGTAGTGAGCGTTGGTACTGGAGCCTTAATAGCTATGGGTTGTGTAATGGTACAT
AAGTGTCATGTAGGCTCATGCCCTACAGGTTTAACTGCCAAGATTGATGGGACTAGAGTAGTTGACGTAGAGTTCGGCGT
TAAGATGCTAGTTAATTTTATCAATGGATTTTCCATGGAGTTAGCTAATATTTTAGATAATTTAGGTTTGAATAGTATTA
AGGAACTTAGAGGTAAAAGAGAGTTACTTTATGGACATGGCTTGAGTAAAGACACGTTAGAAATTCTAGGAATTGAAGGT
ACGGAAGATGAAGTGAATCCTAAATTGGGAGAATTATGGAATAGGAGAGTTATTGCTTACATGCACGAATTGATGAATAA
GGGCAATCCAGTAATAACCAGTATGGGTAGTACTGCACCACCTGATGTAGAGAAACCCGCCAGAATAATTGATTGGCTTA
GATCTGATGGAGCCCAAGTTACCAGACCTTCAATAGATCCTTATAGGGAAGATGTAGATACTTCCTTTTACCTTAAAGGT
GGAGAGATATATCTATCACTCCCAATAATCTTCGATATCACTGAAGCCTCTTTAGAGTATAAGGAAGCGTTTAGTTGGAG
TGCTTTAGCTTTGTCTTCAGCAGTTTTTGACTATGAAACTATCGATAAGTACGCAGAAGTCTTCATAAGTAGTGATGGGA
AAGGTATTGCTAGGTGGAGTAAGAGTGATATCTACGAGAACTCGTATCTCCTGATACCTGCAGATGAGAACGTAATAGAC
GAAGTTATTGGAATGGGCGTTCAAGGGTTTATAGTTGATGAGGATAGTGGAAATAGTGATCTAGAATTGGTAGTTAGTGC
TTTAGATACCAAATTAAAGGAAGTGGGTGTTAGAAATCATTATGACATTTTGGCTAAGTCAAGTAGACTCAGACATTCCG
CAGACGCTTTTAAATTAGTTCTGTTGGGTGCTGATTCTGTAATTATGCCTTATTTTATTTTAGAAAAAGCCATAGGTGAG
GGAAATAAGGGTAATTTGAAAGAGAAAGCCTTCAGTCTCATAGCTGGAATGAAAAAGGAAATAGCTTTGCTTGCCGGTGC
TGCTGGGGTTTATAGTGTCCAATCTACTTTGACCGGTAACAGGGAGTTATTACGTTCTATCAATTTGAGCTATCCTATAA
GGAAAGCACTTAGAATAAAACCAGCGGGGTCTTTATAA

Upstream 100 bases:

>100_bases
AACTATACTTTACAATACTATTTCATATAAACAGTTATATAATATATTTCATTATAGTTATCTTGCGGTTAGCTTGCTCG
TATACAACAAATATCTGCCA

Downstream 100 bases:

>100_bases
TGGTTGACTATTATCCTTCTGGTTGTGGTGTTTTTGGAATCTTAAGGAAAAGAAACTCTCCAAAAGTCAAAGGCAATTTA
GTTGTTAGAGCAATAGATAG

Product: glutamate synthase (NADPH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MPKQFNDEFWTVEKVEHIRYLSLTGKPYKIFNEDMNSLRVLDKVRFKLDKATSISLTPKANLELKFSGIYMKSPLYLGDM
SYGALSGNPNIAIATAADLTETLAGTGEGGLHPEVAKHKRIFVQWASARFGVDIRVLTAGMGVVIKIGQGAKPGIGGHLP
GNKVTEPISVTRRIPIGIDAISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYIVSGIARMGADGVIIDGH
GAGTGATPVVIRDNVGIPIELAVASADNILRREGLRDKFTIIAAGRVSSATDAAKLIALGADVVSVGTGALIAMGCVMVH
KCHVGSCPTGLTAKIDGTRVVDVEFGVKMLVNFINGFSMELANILDNLGLNSIKELRGKRELLYGHGLSKDTLEILGIEG
TEDEVNPKLGELWNRRVIAYMHELMNKGNPVITSMGSTAPPDVEKPARIIDWLRSDGAQVTRPSIDPYREDVDTSFYLKG
GEIYLSLPIIFDITEASLEYKEAFSWSALALSSAVFDYETIDKYAEVFISSDGKGIARWSKSDIYENSYLLIPADENVID
EVIGMGVQGFIVDEDSGNSDLELVVSALDTKLKEVGVRNHYDILAKSSRLRHSADAFKLVLLGADSVIMPYFILEKAIGE
GNKGNLKEKAFSLIAGMKKEIALLAGAAGVYSVQSTLTGNRELLRSINLSYPIRKALRIKPAGSL

Sequences:

>Translated_705_residues
MPKQFNDEFWTVEKVEHIRYLSLTGKPYKIFNEDMNSLRVLDKVRFKLDKATSISLTPKANLELKFSGIYMKSPLYLGDM
SYGALSGNPNIAIATAADLTETLAGTGEGGLHPEVAKHKRIFVQWASARFGVDIRVLTAGMGVVIKIGQGAKPGIGGHLP
GNKVTEPISVTRRIPIGIDAISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYIVSGIARMGADGVIIDGH
GAGTGATPVVIRDNVGIPIELAVASADNILRREGLRDKFTIIAAGRVSSATDAAKLIALGADVVSVGTGALIAMGCVMVH
KCHVGSCPTGLTAKIDGTRVVDVEFGVKMLVNFINGFSMELANILDNLGLNSIKELRGKRELLYGHGLSKDTLEILGIEG
TEDEVNPKLGELWNRRVIAYMHELMNKGNPVITSMGSTAPPDVEKPARIIDWLRSDGAQVTRPSIDPYREDVDTSFYLKG
GEIYLSLPIIFDITEASLEYKEAFSWSALALSSAVFDYETIDKYAEVFISSDGKGIARWSKSDIYENSYLLIPADENVID
EVIGMGVQGFIVDEDSGNSDLELVVSALDTKLKEVGVRNHYDILAKSSRLRHSADAFKLVLLGADSVIMPYFILEKAIGE
GNKGNLKEKAFSLIAGMKKEIALLAGAAGVYSVQSTLTGNRELLRSINLSYPIRKALRIKPAGSL
>Mature_704_residues
PKQFNDEFWTVEKVEHIRYLSLTGKPYKIFNEDMNSLRVLDKVRFKLDKATSISLTPKANLELKFSGIYMKSPLYLGDMS
YGALSGNPNIAIATAADLTETLAGTGEGGLHPEVAKHKRIFVQWASARFGVDIRVLTAGMGVVIKIGQGAKPGIGGHLPG
NKVTEPISVTRRIPIGIDAISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYIVSGIARMGADGVIIDGHG
AGTGATPVVIRDNVGIPIELAVASADNILRREGLRDKFTIIAAGRVSSATDAAKLIALGADVVSVGTGALIAMGCVMVHK
CHVGSCPTGLTAKIDGTRVVDVEFGVKMLVNFINGFSMELANILDNLGLNSIKELRGKRELLYGHGLSKDTLEILGIEGT
EDEVNPKLGELWNRRVIAYMHELMNKGNPVITSMGSTAPPDVEKPARIIDWLRSDGAQVTRPSIDPYREDVDTSFYLKGG
EIYLSLPIIFDITEASLEYKEAFSWSALALSSAVFDYETIDKYAEVFISSDGKGIARWSKSDIYENSYLLIPADENVIDE
VIGMGVQGFIVDEDSGNSDLELVVSALDTKLKEVGVRNHYDILAKSSRLRHSADAFKLVLLGADSVIMPYFILEKAIGEG
NKGNLKEKAFSLIAGMKKEIALLAGAAGVYSVQSTLTGNRELLRSINLSYPIRKALRIKPAGSL

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=307, Percent_Identity=35.8306188925081, Blast_Score=161, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17570289, Length=324, Percent_Identity=37.037037037037, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320030, Length=314, Percent_Identity=35.6687898089172, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28574881, Length=320, Percent_Identity=35, Blast_Score=167, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24665539, Length=320, Percent_Identity=35, Blast_Score=167, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 76220; Mature: 76089

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQFNDEFWTVEKVEHIRYLSLTGKPYKIFNEDMNSLRVLDKVRFKLDKATSISLTPKA
CCCCCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
NLELKFSGIYMKSPLYLGDMSYGALSGNPNIAIATAADLTETLAGTGEGGLHPEVAKHKR
CEEEEEEEEEEECCEEECCCCCCEECCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHCCE
IFVQWASARFGVDIRVLTAGMGVVIKIGQGAKPGIGGHLPGNKVTEPISVTRRIPIGIDA
EEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCEEC
ISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYIVSGIARMGADGVIIDGH
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECC
GAGTGATPVVIRDNVGIPIELAVASADNILRREGLRDKFTIIAAGRVSSATDAAKLIALG
CCCCCCCCEEEECCCCCEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCC
ADVVSVGTGALIAMGCVMVHKCHVGSCPTGLTAKIDGTRVVDVEFGVKMLVNFINGFSME
CHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHCCCHHH
LANILDNLGLNSIKELRGKRELLYGHGLSKDTLEILGIEGTEDEVNPKLGELWNRRVIAY
HHHHHHHCCHHHHHHHCCCHHEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
MHELMNKGNPVITSMGSTAPPDVEKPARIIDWLRSDGAQVTRPSIDPYREDVDTSFYLKG
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHCCCCEEEEEC
GEIYLSLPIIFDITEASLEYKEAFSWSALALSSAVFDYETIDKYAEVFISSDGKGIARWS
CEEEEEEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCC
KSDIYENSYLLIPADENVIDEVIGMGVQGFIVDEDSGNSDLELVVSALDTKLKEVGVRNH
CCCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
YDILAKSSRLRHSADAFKLVLLGADSVIMPYFILEKAIGEGNKGNLKEKAFSLIAGMKKE
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IALLAGAAGVYSVQSTLTGNRELLRSINLSYPIRKALRIKPAGSL
HHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCHHHEEECCCCCC
>Mature Secondary Structure 
PKQFNDEFWTVEKVEHIRYLSLTGKPYKIFNEDMNSLRVLDKVRFKLDKATSISLTPKA
CCCCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
NLELKFSGIYMKSPLYLGDMSYGALSGNPNIAIATAADLTETLAGTGEGGLHPEVAKHKR
CEEEEEEEEEEECCEEECCCCCCEECCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHCCE
IFVQWASARFGVDIRVLTAGMGVVIKIGQGAKPGIGGHLPGNKVTEPISVTRRIPIGIDA
EEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCEEC
ISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYIVSGIARMGADGVIIDGH
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECC
GAGTGATPVVIRDNVGIPIELAVASADNILRREGLRDKFTIIAAGRVSSATDAAKLIALG
CCCCCCCCEEEECCCCCEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCC
ADVVSVGTGALIAMGCVMVHKCHVGSCPTGLTAKIDGTRVVDVEFGVKMLVNFINGFSME
CHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHCCCHHH
LANILDNLGLNSIKELRGKRELLYGHGLSKDTLEILGIEGTEDEVNPKLGELWNRRVIAY
HHHHHHHCCHHHHHHHCCCHHEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
MHELMNKGNPVITSMGSTAPPDVEKPARIIDWLRSDGAQVTRPSIDPYREDVDTSFYLKG
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHCCCCEEEEEC
GEIYLSLPIIFDITEASLEYKEAFSWSALALSSAVFDYETIDKYAEVFISSDGKGIARWS
CEEEEEEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCC
KSDIYENSYLLIPADENVIDEVIGMGVQGFIVDEDSGNSDLELVVSALDTKLKEVGVRNH
CCCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
YDILAKSSRLRHSADAFKLVLLGADSVIMPYFILEKAIGEGNKGNLKEKAFSLIAGMKKE
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
IALLAGAAGVYSVQSTLTGNRELLRSINLSYPIRKALRIKPAGSL
HHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]