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The map label for this gene is speE

Identifier: 227827658

GI number: 227827658

Start: 1310505

End: 1311410

Strand: Direct

Name: speE

Synonym: M1425_1386

Alternate gene names: 227827658

Gene position: 1310505-1311410 (Clockwise)

Preceding gene: 227827657

Following gene: 227827660

Centisome position: 50.23

GC content: 37.31

Gene sequence:

>906_bases
ATGTTTGGTTGGCATTGGCTTCTAGAATGGCAAACGCCATACGAGTTTCACGGTCACCTAATAGAAAAAGTATTCGCTGA
AGAGAAGACCCCGTACCAACACGTTACATTAGTAGAATTTACCAGGTTTGGAAAAGGACTTATAATAGATGGAAAAGTGC
AATCAACTCTTTACGACGAGCACATTTATCATGAATTGTTAGTTCACCCCTTATTGCTATCATTGCCAAAACCTCCTAAG
AATGTGCTAATTTTAGGTGGCGGAGAGGGTGCAACTCTTAGGGAGGTTCTTAAGTATAAGTCTGTAGAAAAAGCAGTAAT
GGTTGATATAGACGAGAAGGTTATAGAATTTGCTAAGAAATATTTATATGAATGGCATCAAGGAGCTTTTGAGGATAAGA
GGACTAGCCTAGTAATTACTGATGGTCTAAAGTTTATAAATGAAACAAAGGATAAATACGACGCTATAATTCTAGATCTA
ACAGATCCGATCAAGGATTCCACGTCATACATGCTTTACACTAAGGAGTTCTATGAGAAATTGAGAGGAATATTAAATCA
AGGAGGTGGTATAGTAACACAAGCTACATCTCCATCATTTTCTCTAGAAGTATATGTGACGATTTACAATACTATAAAGG
AGGTGTTTAAGGAAGCCTCAGCTTCATACACATATATGGCATCCTTTGATGGATTATGGGGATTCGTTTATGGGGGAGTA
AGGCCAGACTTACTTTCGGAGGATGAAGTAAATTCCAGAATAAGGGAACGTATTAGTGGACAGCTAAGGTTCTATGATGA
CTATTCTCATAAGATTTCGTTTAGTTTGCCTAAGAATATAAAAAGTGAATTCAAGAAGATAACCAAAGTATCCACGGAGA
AAGATCCGATTTACGTTCCTGCGTAA

Upstream 100 bases:

>100_bases
TAAGTAAGCACTACTGTGCAGCTTGTGGATTTGGAAAGACGAAGAAGATAAGGAGATACAGTTGGCAAAATAAAAAGGTT
AATGGGGTCAGAATAAGATA

Downstream 100 bases:

>100_bases
TTTTTTAAATTTTTATCGATATATATTTAATTGCCGGGGTGCCCGAGCGGTCAAGGGGGTAGGCTCGAGACCTTTCCAGC
GTTGCTGCGAGGGACCTACT

Product: Spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MFGWHWLLEWQTPYEFHGHLIEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLPKPPK
NVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTSLVITDGLKFINETKDKYDAIILDL
TDPIKDSTSYMLYTKEFYEKLRGILNQGGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVNSRIRERISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEKDPIYVPA

Sequences:

>Translated_301_residues
MFGWHWLLEWQTPYEFHGHLIEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLPKPPK
NVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTSLVITDGLKFINETKDKYDAIILDL
TDPIKDSTSYMLYTKEFYEKLRGILNQGGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVNSRIRERISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEKDPIYVPA
>Mature_301_residues
MFGWHWLLEWQTPYEFHGHLIEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLPKPPK
NVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTSLVITDGLKFINETKDKYDAIILDL
TDPIKDSTSYMLYTKEFYEKLRGILNQGGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVNSRIRERISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEKDPIYVPA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=270, Percent_Identity=27.4074074074074, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786312, Length=285, Percent_Identity=35.4385964912281, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25154106, Length=264, Percent_Identity=34.469696969697, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI212645616, Length=233, Percent_Identity=36.480686695279, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325326, Length=258, Percent_Identity=33.7209302325581, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323175, Length=265, Percent_Identity=31.3207547169811, Blast_Score=138, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24645443, Length=285, Percent_Identity=31.5789473684211, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355941, Length=222, Percent_Identity=31.5315315315315, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_SULIA (C3N5P4)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843364.1
- ProteinModelPortal:   C3N5P4
- GeneID:   7814198
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1436
- OMA:   VEGGPCY
- ProtClustDB:   CLSK883670
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 34770; Mature: 34770

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 89-89 BINDING 109-109 BINDING 159-159 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGWHWLLEWQTPYEFHGHLIEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDE
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHCCCCEEECCCHHHHHHHH
HIYHELLVHPLLLSLPKPPKNVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKK
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHEEEECHHHHHHHHHH
YLYEWHQGAFEDKRTSLVITDGLKFINETKDKYDAIILDLTDPIKDSTSYMLYTKEFYEK
HHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHH
LRGILNQGGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
HHHHHHCCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCC
RPDLLSEDEVNSRIRERISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEKDPIYVP
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEC
A
C
>Mature Secondary Structure
MFGWHWLLEWQTPYEFHGHLIEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDE
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHCCCCEEECCCHHHHHHHH
HIYHELLVHPLLLSLPKPPKNVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKK
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHEEEECHHHHHHHHHH
YLYEWHQGAFEDKRTSLVITDGLKFINETKDKYDAIILDLTDPIKDSTSYMLYTKEFYEK
HHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHH
LRGILNQGGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
HHHHHHCCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCC
RPDLLSEDEVNSRIRERISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEKDPIYVP
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA