Click here to switch to the map view.

The map label for this gene is tfbB [H]

Identifier: 227827554

GI number: 227827554

Start: 1217917

End: 1218798

Strand: Direct

Name: tfbB [H]

Synonym: M1425_1281

Alternate gene names: 227827554

Gene position: 1217917-1218798 (Clockwise)

Preceding gene: 227827553

Following gene: 227827555

Centisome position: 46.68

GC content: 35.03

Gene sequence:

>882_bases
ATGAAATGTCCATTTTGTGGAACGGAGGACACAATAACATATGATATGGAAAAGGGAATGTACGTATGTACAAACTGCGG
CAGTGTAATAGAAGATAATGCAGTAGATCCAGGTCCAGATTGGAGAGCCTATAATGCAAAAGACAGAAATGAGAAAGAGA
GAGTAGGTAGCCCAAGTACTCCAAAAGTACATGACTGGGGATTTCATACTATAATAGGATACGGTAGAGCTAAGGACAGA
CTAAAAACATTAAAGATGCAAAGAATGCAAAATAAGATACGTGTGTCACCTAAGGATAAAAAGCTAGTCACCTTACTTTC
TATTTTAAATGATGAGAGTTCAAAATTAGAGTTACCAGAACATGTCAAAGAAACTGCATCGTTAATAATAAGGAAAATGG
TAGAAACTGGACAAACCAAAAGGATAGATCAGTATACGCTAATAGTTGCTGCCTTATATTATTCTTGCCAAGTGAATAAC
GTACCAAGACACCTCCAAGAGTTTAAAGTTAGATATTCCATATCTTCTAGCGAATTTTGGAACGCCTTAAAGAGAGTTCA
ATCTGTTGCAAAATCAATACCGGACTTTCGTCCTAAAATCAGACCAACAGAATATATACCAAAAATAATATACAGATTAA
ATTTACCACCAATTATAGGAACAAAGGCATCCGAGTTAGTAGACTTAATGCATAAACAAGGGCTGACTAGCGGAAAAGGA
TACTTATCTTTAAGCGCAGCTTCGGTTTACTTAATATCGGCACTAATGGATATAAAAAAGACGCAGAAAGAGGTTGCAGA
CTCGTTAGATATTACTGAAGTAACTATTAGGAATAGATATAAGGATATAGTGGATAATTTTGATATTATTGTTACATTAT
GA

Upstream 100 bases:

>100_bases
ACGTAAAAGAACCTTATGCTTTAGCTATGTTAGATAAAAATTCAAAAATTTTAGTGAACGAGAAAATATTCGTTAGTTAT
AGTGAGAGGAGGGCAAGAAA

Downstream 100 bases:

>100_bases
TTTTTATAGAATTATATAAAAGTATTGTGTATAGTATTTGCTCATTTTAAATCTTATTTATTATTAAAACTATTGAAAAG
TAGTCAATTGTCACTAAAGA

Product: Zinc finger TFIIB-type domain protein

Products: NA

Alternate protein names: TFIIB 2 [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKCPFCGTEDTITYDMEKGMYVCTNCGSVIEDNAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGQTKRIDQYTLIVAALYYSCQVNN
VPRHLQEFKVRYSISSSEFWNALKRVQSVAKSIPDFRPKIRPTEYIPKIIYRLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIIVTL

Sequences:

>Translated_293_residues
MKCPFCGTEDTITYDMEKGMYVCTNCGSVIEDNAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGQTKRIDQYTLIVAALYYSCQVNN
VPRHLQEFKVRYSISSSEFWNALKRVQSVAKSIPDFRPKIRPTEYIPKIIYRLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIIVTL
>Mature_293_residues
MKCPFCGTEDTITYDMEKGMYVCTNCGSVIEDNAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDR
LKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGQTKRIDQYTLIVAALYYSCQVNN
VPRHLQEFKVRYSISSSEFWNALKRVQSVAKSIPDFRPKIRPTEYIPKIIYRLNLPPIIGTKASELVDLMHKQGLTSGKG
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIIVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=268, Percent_Identity=24.6268656716418, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17564892, Length=272, Percent_Identity=25.7352941176471, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6325343, Length=303, Percent_Identity=24.4224422442244, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17136758, Length=280, Percent_Identity=24.6428571428571, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 33411; Mature: 33411

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCPFCGTEDTITYDMEKGMYVCTNCGSVIEDNAVDPGPDWRAYNAKDRNEKERVGSPST
CCCCCCCCCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
PKVHDWGFHTIIGYGRAKDRLKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPE
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
HVKETASLIIRKMVETGQTKRIDQYTLIVAALYYSCQVNNVPRHLQEFKVRYSISSSEFW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
NALKRVQSVAKSIPDFRPKIRPTEYIPKIIYRLNLPPIIGTKASELVDLMHKQGLTSGKG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIIVTL
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKCPFCGTEDTITYDMEKGMYVCTNCGSVIEDNAVDPGPDWRAYNAKDRNEKERVGSPST
CCCCCCCCCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
PKVHDWGFHTIIGYGRAKDRLKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPE
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
HVKETASLIIRKMVETGQTKRIDQYTLIVAALYYSCQVNNVPRHLQEFKVRYSISSSEFW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
NALKRVQSVAKSIPDFRPKIRPTEYIPKIIYRLNLPPIIGTKASELVDLMHKQGLTSGKG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
YLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIIVTL
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]