Click here to switch to the map view.

The map label for this gene is hyfB [H]

Identifier: 227827481

GI number: 227827481

Start: 1135895

End: 1137640

Strand: Direct

Name: hyfB [H]

Synonym: M1425_1203

Alternate gene names: 227827481

Gene position: 1135895-1137640 (Clockwise)

Preceding gene: 227827480

Following gene: 227827482

Centisome position: 43.54

GC content: 35.34

Gene sequence:

>1746_bases
ATGAACACGCTACTCTATACATTACCCCCATTTCTAATTTCTATTATATTCTCAATCTTTAATAAAAAAGCGGGATATAT
CTTGACAATGATATCATCAATAATACTACTTATCACCTCGATTTACGAATATAATGGAGTTCTCTCATTCTTCTCCATAA
TTTCTACCTCAGTGTGGATTCTCGCCTCAATTTTCTCAATAGAGTACGATCATTACGGGAAATGGCTCTCCCCCTTATAC
ATACTAACAATCTTAGGCATGGCCATTGTCTTAAACTCTGATGGTTATCTGTTATTCCTCGCAGGTTGGGAAATAATGAC
AATCCCAGCATATGTAGCAATAGGTCTAACAGTAAAGAATAATAGACCACCATTCGTTTTCATGGCATTTGGAGAGCTGA
GTACAGTTCTCATTTTAGCGGGATTTATAATAGCAAACACTACAAACTTCAATTACTTGCCATCTCCAGTTCCCTTGATT
GTCGCTACTTTTGGATTCATAATAAAAATGGGAATGATGCCTTTCCTTGTATCGGAATGGCTACCTATAGCACATGGAAC
AGCACCATCAAACTTTTCTGCAATACTAAGTGCCACAATGACGCTAATGGGGGTTTACGGAATACTAAAAATTACAATTT
TAACACAAACTATTCCAATTGGATTTTCACTTACAATAATGGCCATAGGTGCATTTAGCATATTCTTTGGAGCACTTTAC
GGATACGTAAATGAGAACACTAAGGGAATTTTAGCCTTTAGTACAATAGAAAATAACGGGGCAATACTAGTAGCCTTAAG
TCTATACATGATCTCAAAGCAACTAAACATTACCTCAATAGAGCACATTTCCTTAATCACTGTAATTTTATACTCATTCG
CACACTCAGTAGCCAAAACTGGACTATTCCTCTCTTCTGGCTTACAAGAAAAACAGAGCATAACTTATTCAAAAAAGATA
AGAAACATTGAAATAGGACTAACCCTATTGGCTTCCTCCATGTCTGGACTATTGCCGAATATAGGGGGAGTAGCATCTTG
GCTATTACTTGAAAACCTATTCATGTTCTCATACGTACTTCATAACGTGATATCTATCCTCTTCATAATGACTGGAGCAA
TTATCGCAATGGGAGAAGGTCTAGCAACAGCCTTATTAATTAGATACATAACGTATACATCAATATTTCAGAATACAAGA
GAACAATTAAGTAAAATAAAGAAATATCCCATCTTATTATCTGCACTTATAGTACTCATACTGGGATTCACGTTACCTTA
TTTGATATACCCTTATAAAAACTCTACAACAATTCTAGGAATGCTAACCAATAGCGTGATTCTAACACAATACTATAACA
CCACATTCGGAGGAATATCGCCACTCTACATAATTTTACTATTAGTAGCCTTCTCTATTGTAAGCTATCTCGCCTTTGGA
AAGCCTAAAATAAGAAAAGCAGAAACGTGGAACAATGGGGTAAACGAGAAAGAAGAATACACAGCATTTGCAATGGCAAA
TAACATAAGACAAATGCTAAAGAAAATCTTAAGGCCAGAAGAAGAGAAATTCTTACCTACATACGGTTTAGATGTATTCT
GGGAATACCTCTACAAACTAGCAAATTCAATAAGAAGATTTGGGAAGATTTTCGCTAAAACACTTATCAACAGCTCAATC
TCATGGTACATTATATACATTATTTTGACGCTCATTGTAGTAATAATAGTAGTTGTAATGGGGTGA

Upstream 100 bases:

>100_bases
TAATAGGCGAAGGAGTACCATTAGAAGCAGTAGTGGAAATCCCCGGTTGTCCACCAAATCCCTTTACAATACTCGAAGCC
TTGATGAAGGTGAAGGGAAA

Downstream 100 bases:

>100_bases
AACTATTACATGTTCAAAAGAAAAAGAACTAAAGGAGAAAATAGAGGAGAAGATAAAGAGAATAGAGGAACTGAAGAGGA
GAATAGAGGAAACGAGAAGA

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MNTLLYTLPPFLISIIFSIFNKKAGYILTMISSIILLITSIYEYNGVLSFFSIISTSVWILASIFSIEYDHYGKWLSPLY
ILTILGMAIVLNSDGYLLFLAGWEIMTIPAYVAIGLTVKNNRPPFVFMAFGELSTVLILAGFIIANTTNFNYLPSPVPLI
VATFGFIIKMGMMPFLVSEWLPIAHGTAPSNFSAILSATMTLMGVYGILKITILTQTIPIGFSLTIMAIGAFSIFFGALY
GYVNENTKGILAFSTIENNGAILVALSLYMISKQLNITSIEHISLITVILYSFAHSVAKTGLFLSSGLQEKQSITYSKKI
RNIEIGLTLLASSMSGLLPNIGGVASWLLLENLFMFSYVLHNVISILFIMTGAIIAMGEGLATALLIRYITYTSIFQNTR
EQLSKIKKYPILLSALIVLILGFTLPYLIYPYKNSTTILGMLTNSVILTQYYNTTFGGISPLYIILLLVAFSIVSYLAFG
KPKIRKAETWNNGVNEKEEYTAFAMANNIRQMLKKILRPEEEKFLPTYGLDVFWEYLYKLANSIRRFGKIFAKTLINSSI
SWYIIYIILTLIVVIIVVVMG

Sequences:

>Translated_581_residues
MNTLLYTLPPFLISIIFSIFNKKAGYILTMISSIILLITSIYEYNGVLSFFSIISTSVWILASIFSIEYDHYGKWLSPLY
ILTILGMAIVLNSDGYLLFLAGWEIMTIPAYVAIGLTVKNNRPPFVFMAFGELSTVLILAGFIIANTTNFNYLPSPVPLI
VATFGFIIKMGMMPFLVSEWLPIAHGTAPSNFSAILSATMTLMGVYGILKITILTQTIPIGFSLTIMAIGAFSIFFGALY
GYVNENTKGILAFSTIENNGAILVALSLYMISKQLNITSIEHISLITVILYSFAHSVAKTGLFLSSGLQEKQSITYSKKI
RNIEIGLTLLASSMSGLLPNIGGVASWLLLENLFMFSYVLHNVISILFIMTGAIIAMGEGLATALLIRYITYTSIFQNTR
EQLSKIKKYPILLSALIVLILGFTLPYLIYPYKNSTTILGMLTNSVILTQYYNTTFGGISPLYIILLLVAFSIVSYLAFG
KPKIRKAETWNNGVNEKEEYTAFAMANNIRQMLKKILRPEEEKFLPTYGLDVFWEYLYKLANSIRRFGKIFAKTLINSSI
SWYIIYIILTLIVVIIVVVMG
>Mature_581_residues
MNTLLYTLPPFLISIIFSIFNKKAGYILTMISSIILLITSIYEYNGVLSFFSIISTSVWILASIFSIEYDHYGKWLSPLY
ILTILGMAIVLNSDGYLLFLAGWEIMTIPAYVAIGLTVKNNRPPFVFMAFGELSTVLILAGFIIANTTNFNYLPSPVPLI
VATFGFIIKMGMMPFLVSEWLPIAHGTAPSNFSAILSATMTLMGVYGILKITILTQTIPIGFSLTIMAIGAFSIFFGALY
GYVNENTKGILAFSTIENNGAILVALSLYMISKQLNITSIEHISLITVILYSFAHSVAKTGLFLSSGLQEKQSITYSKKI
RNIEIGLTLLASSMSGLLPNIGGVASWLLLENLFMFSYVLHNVISILFIMTGAIIAMGEGLATALLIRYITYTSIFQNTR
EQLSKIKKYPILLSALIVLILGFTLPYLIYPYKNSTTILGMLTNSVILTQYYNTTFGGISPLYIILLLVAFSIVSYLAFG
KPKIRKAETWNNGVNEKEEYTAFAMANNIRQMLKKILRPEEEKFLPTYGLDVFWEYLYKLANSIRRFGKIFAKTLINSSI
SWYIIYIILTLIVVIIVVVMG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=282, Percent_Identity=30.1418439716312, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 64716; Mature: 64716

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLLYTLPPFLISIIFSIFNKKAGYILTMISSIILLITSIYEYNGVLSFFSIISTSVWI
CCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASIFSIEYDHYGKWLSPLYILTILGMAIVLNSDGYLLFLAGWEIMTIPAYVAIGLTVKN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCEEEEECCHHHEEHHHHEEEEEEEC
NRPPFVFMAFGELSTVLILAGFIIANTTNFNYLPSPVPLIVATFGFIIKMGMMPFLVSEW
CCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LPIAHGTAPSNFSAILSATMTLMGVYGILKITILTQTIPIGFSLTIMAIGAFSIFFGALY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GYVNENTKGILAFSTIENNGAILVALSLYMISKQLNITSIEHISLITVILYSFAHSVAKT
HHHCCCCCEEEEEEEECCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLFLSSGLQEKQSITYSKKIRNIEIGLTLLASSMSGLLPNIGGVASWLLLENLFMFSYVL
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
HNVISILFIMTGAIIAMGEGLATALLIRYITYTSIFQNTREQLSKIKKYPILLSALIVLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFTLPYLIYPYKNSTTILGMLTNSVILTQYYNTTFGGISPLYIILLLVAFSIVSYLAFG
HHHHHHHEEEECCCCCEEEEEHHCHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KPKIRKAETWNNGVNEKEEYTAFAMANNIRQMLKKILRPEEEKFLPTYGLDVFWEYLYKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ANSIRRFGKIFAKTLINSSISWYIIYIILTLIVVIIVVVMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLLYTLPPFLISIIFSIFNKKAGYILTMISSIILLITSIYEYNGVLSFFSIISTSVWI
CCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASIFSIEYDHYGKWLSPLYILTILGMAIVLNSDGYLLFLAGWEIMTIPAYVAIGLTVKN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCEEEEECCHHHEEHHHHEEEEEEEC
NRPPFVFMAFGELSTVLILAGFIIANTTNFNYLPSPVPLIVATFGFIIKMGMMPFLVSEW
CCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LPIAHGTAPSNFSAILSATMTLMGVYGILKITILTQTIPIGFSLTIMAIGAFSIFFGALY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GYVNENTKGILAFSTIENNGAILVALSLYMISKQLNITSIEHISLITVILYSFAHSVAKT
HHHCCCCCEEEEEEEECCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLFLSSGLQEKQSITYSKKIRNIEIGLTLLASSMSGLLPNIGGVASWLLLENLFMFSYVL
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
HNVISILFIMTGAIIAMGEGLATALLIRYITYTSIFQNTREQLSKIKKYPILLSALIVLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFTLPYLIYPYKNSTTILGMLTNSVILTQYYNTTFGGISPLYIILLLVAFSIVSYLAFG
HHHHHHHEEEECCCCCEEEEEHHCHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KPKIRKAETWNNGVNEKEEYTAFAMANNIRQMLKKILRPEEEKFLPTYGLDVFWEYLYKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ANSIRRFGKIFAKTLINSSISWYIIYIILTLIVVIIVVVMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]