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The map label for this gene is hycE [C]

Identifier: 227827479

GI number: 227827479

Start: 1134121

End: 1135296

Strand: Direct

Name: hycE [C]

Synonym: M1425_1201

Alternate gene names: 227827479

Gene position: 1134121-1135296 (Clockwise)

Preceding gene: 227827478

Following gene: 227827480

Centisome position: 43.47

GC content: 37.16

Gene sequence:

>1176_bases
ATGAAATACTATAAATGGACTCAAAAAGGCGAGGGAAGGAAAATAGGCAAAATAGGGGATTATTGTCTCTACGAAAAAAC
GATAACAGAAGAGAAATGTGAGGAAAACAAACCAAATATAACACAAACGTATGGATCATTCAAGTTCATTTACGGACCCT
CAGCTGGAGGACTACTCGAAACAATAAAATTCATTATTACAACTAATGGTGAAAAAATTCTAGGAATAGACGCTGAGGTA
TACAAGAACAGAGAAATAGTAATAAGCGGTTTAACTGTGGACGATGCCTTACTCAGAGTAGAGAGAATAAACGCTCCATT
TAGTGCTTCCCACACAATATCCTTTTTACTCGCTGTAGAAGATTCGTTAGAATTAGAACAAGACTATCCAACCCAACTAA
AGAGAATAGCCGAAATAGAATTGGAAAGAATAAGAAATCACTTATTCGTAATATCGAGATTAACTGAAACCGCATCACTA
AACGTACCTACATACCATCTCTTGCACCTCGTTGAAAAAGTCAACAGATTAATAGGCAAAATGTGTGGTCACAGGTATTT
CTTTGGCGTTAATGCAATTAACGGGGTTAACTGTGATTTCGGAAATTTATTAAGAATAATAGATATTACCAAGGAATTTA
AACAAATCTTCGATGGGCTACTTGAAAGTAGAATCTTCATAGATAGACTCCAAGAAAACGGAAAAATAATAGATGAAAAC
AGTATAGGACCAGCTGCAAGAGCTGCTGGACTCGCTTACGATGCGAGAAAGGACTTTAAAGCCTTACCTTATGAAGACTT
AGGTTTTAGAACAGTTATCACACAAGAGGCAGACTCATTCGGAAGGTTCCTAGTTAGGGGAATGGAGATAATCGAGTCGG
CCAAAATTTTAGTAGAGTTACACGATGAAATAAAGAACAGCAATAACGAGAGAGGGAAAAATCACAAACAAGGAGGGGGA
GAGGGACTAGCCAGAGTCGAGAGTCCATCTGGTGATCTAGCCTATTACGTCAAGTTAAATAACGGGATTATCGACTCAGT
ATCACTTCTCACTCCTTCACAAGTCAATCTCAACCTATTTTTGAAAAGCGTGATAAACACAATATTTACCGATTTTCAAT
TCAATTGGGAAAGTTTTGGAATTTGGGTAAGTGAAATAGGAGTGATGTTAAAGTGA

Upstream 100 bases:

>100_bases
AGCTGACAGTCTCAGAAGAACCCAAGCTAATCACGTTCATTTCTCTTGTATCATCTATAATTCCACTCGTTCTCGGTATA
CTTTTACTGGTGATCCTTTT

Downstream 100 bases:

>100_bases
AGAATTGGTGGTTTATTAGAGGTTTAAGAAAAGGAGTAATGACAGAGAAATACCCGAAGGAAATAGCGGAATGGAGCACT
GAAATACAAGGAGAGGGAAC

Product: formate hydrogenlyase subunit 5 (HycE)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLETIKFIITTNGEKILGIDAEV
YKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVEDSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETASL
NVPTYHLLHLVEKVNRLIGKMCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDEN
SIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVELHDEIKNSNNERGKNHKQGGG
EGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLFLKSVINTIFTDFQFNWESFGIWVSEIGVMLK

Sequences:

>Translated_391_residues
MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLETIKFIITTNGEKILGIDAEV
YKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVEDSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETASL
NVPTYHLLHLVEKVNRLIGKMCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDEN
SIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVELHDEIKNSNNERGKNHKQGGG
EGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLFLKSVINTIFTDFQFNWESFGIWVSEIGVMLK
>Mature_391_residues
MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLETIKFIITTNGEKILGIDAEV
YKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVEDSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETASL
NVPTYHLLHLVEKVNRLIGKMCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDEN
SIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVELHDEIKNSNNERGKNHKQGGG
EGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLFLKSVINTIFTDFQFNWESFGIWVSEIGVMLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Drosophila melanogaster, GI221459469, Length=281, Percent_Identity=23.1316725978648, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 44224; Mature: 44224

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCEEECCCEEEEECCCCCCHHH
TIKFIITTNGEKILGIDAEVYKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVE
HEEEEEECCCCEEEECCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETASLNVPTYHLLHLVEKVNRLIGK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDEN
HHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
SIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVEL
CCCCHHHHHCCCHHCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
HDEIKNSNNERGKNHKQGGGEGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLF
HHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEECCCCCCHHHHH
LKSVINTIFTDFQFNWESFGIWVSEIGVMLK
HHHHHHHHHHCCEECHHHHCEEHHHHCCCCC
>Mature Secondary Structure
MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCEEECCCEEEEECCCCCCHHH
TIKFIITTNGEKILGIDAEVYKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVE
HEEEEEECCCCEEEECCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETASLNVPTYHLLHLVEKVNRLIGK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDEN
HHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
SIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVEL
CCCCHHHHHCCCHHCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
HDEIKNSNNERGKNHKQGGGEGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLF
HHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEECCCCCCHHHHH
LKSVINTIFTDFQFNWESFGIWVSEIGVMLK
HHHHHHHHHHCCEECHHHHCEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]