Click here to switch to the map view.

The map label for this gene is fumC [H]

Identifier: 227827427

GI number: 227827427

Start: 1084766

End: 1086103

Strand: Direct

Name: fumC [H]

Synonym: M1425_1146

Alternate gene names: 227827427

Gene position: 1084766-1086103 (Clockwise)

Preceding gene: 227827423

Following gene: 227827431

Centisome position: 41.58

GC content: 35.8

Gene sequence:

>1338_bases
ATGGATTACGTTCTTTTACTTATGAAATATACCGATACTGCGCCCAAGCTTTTTATGAATACCGGAACCAGGTTTCCTAG
AAGAATTATTTGGGCTATGGGAGTTTTAAAGAAATCTTGCGCCAAAGTGAACGCAGATCTTGGATTATTAGACAAAAAGA
TTGCGAATTCAATTATTAAGGCATCTGAAGATTTAATTGATGGAAAATTAGATGATAAAATAGTACTTGATGTCTTTCAA
ACGGGATCTGGGACTGGACTTAATATGAACGTAAATGAGGTTATAGCAGAAGTAGCTTCTAGTTACTCTAACCTTAAAGT
GCATCCAAATGATCATGTGAATTTCGGCCAGTCATCGAATGATACTGTACCTACAGCAATTAGAATCGCTGCAGTAGCTG
AGGTCACGAATAAATTACTTCCTGCATTACAACAAATAATATCTTCTTTAAATAAGAAGGCGGAGGAATATAAGGATGTT
GTAAAAGCAGGTAGAACCCATTTAAGAGATGCTTTGCCTGTAACTTTAGGTCAAGAACTTTCAGCATATGCTGATGCCTT
CCAACATGAACGCGAGCAAGTTATGAATATTTTGGAATATGTAAAGGAGTTGCCAATTGGAGGTACTGCAACGGGTACTG
GACTAAATACTCACCCTGAATTTCAAGAGAGAGTTATTAATGAAATAAACAGAATCACTGGTTTAGGATTTAAGCCAGCT
AATAGGTTTAGGGCAATGAGATTACTCACGGATCTCTTACTATTAAGTGGAGCACTGAGAAATATTGCAGTAAACTTGTA
CAGATTAGGACAAGATATAAGGTTAATGTTTTCCGGTCCTTTAACTGGTCTTAACGAAATAGATTTACCCACACAAGAAG
AGATTGCTGGTAGCTCGATAATGCCTGGTAAAACTAATCCAGTTACAGTTGAGGCTACTTTACTAATTTCAGCCCAAGTT
GTGGGATTGGATCATGCAAATCAATTCGCTTCAATGTTAGGGGAATTTGAGTTATCAATGGGTATTCCATTAGTTGGTTA
TAATATTGTAACTCAAGTTAATTTCGTCTCTGAGGCTTTAGAGAAGATGTCAAAATTAGTAATTGATGGAATGGTAGCAA
ATGTGGAGAAAATGAAGAGATATGCTGAATCGTCTCCTTCACTTATAACCATAGTATCTCCAGTAATAGGCTATGATAAA
GCTTCTGAAATAGGAAAGAAGTTAAATAAGGGAATGTCTATACGTGAAGCTTTAAGGGAATTAGGATATAGTGATAATGA
AATAAATAAAATATTAGACTTAAGTAAACTAGTTAAGCCTGGATTCACTGCCAAATGA

Upstream 100 bases:

>100_bases
GGTAACATTTTCAAGTCTTATTACGGGCATATACATTGGGTATTACGTAAAGTGGGTTAAAGATTTTTTCAAAGATAATT
GCTTATAAAGATTTAATACA

Downstream 100 bases:

>100_bases
TAAAGGATAGAGGGAAACTTCGCCCTTTCAAAGCATGAAGTACTGGATTATCTTTTTTTAACTCCTTCAATTATCCAAAA
TCCTTCCTTCTCCTTGACCA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MDYVLLLMKYTDTAPKLFMNTGTRFPRRIIWAMGVLKKSCAKVNADLGLLDKKIANSIIKASEDLIDGKLDDKIVLDVFQ
TGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSNDTVPTAIRIAAVAEVTNKLLPALQQIISSLNKKAEEYKDV
VKAGRTHLRDALPVTLGQELSAYADAFQHEREQVMNILEYVKELPIGGTATGTGLNTHPEFQERVINEINRITGLGFKPA
NRFRAMRLLTDLLLLSGALRNIAVNLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSIMPGKTNPVTVEATLLISAQV
VGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFVSEALEKMSKLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDK
ASEIGKKLNKGMSIREALRELGYSDNEINKILDLSKLVKPGFTAK

Sequences:

>Translated_445_residues
MDYVLLLMKYTDTAPKLFMNTGTRFPRRIIWAMGVLKKSCAKVNADLGLLDKKIANSIIKASEDLIDGKLDDKIVLDVFQ
TGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSNDTVPTAIRIAAVAEVTNKLLPALQQIISSLNKKAEEYKDV
VKAGRTHLRDALPVTLGQELSAYADAFQHEREQVMNILEYVKELPIGGTATGTGLNTHPEFQERVINEINRITGLGFKPA
NRFRAMRLLTDLLLLSGALRNIAVNLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSIMPGKTNPVTVEATLLISAQV
VGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFVSEALEKMSKLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDK
ASEIGKKLNKGMSIREALRELGYSDNEINKILDLSKLVKPGFTAK
>Mature_445_residues
MDYVLLLMKYTDTAPKLFMNTGTRFPRRIIWAMGVLKKSCAKVNADLGLLDKKIANSIIKASEDLIDGKLDDKIVLDVFQ
TGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSNDTVPTAIRIAAVAEVTNKLLPALQQIISSLNKKAEEYKDV
VKAGRTHLRDALPVTLGQELSAYADAFQHEREQVMNILEYVKELPIGGTATGTGLNTHPEFQERVINEINRITGLGFKPA
NRFRAMRLLTDLLLLSGALRNIAVNLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSIMPGKTNPVTVEATLLISAQV
VGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFVSEALEKMSKLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDK
ASEIGKKLNKGMSIREALRELGYSDNEINKILDLSKLVKPGFTAK

Specific function: May function as an auxiliary aerobic enzyme in the citric acid cycle [H]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=425, Percent_Identity=42.5882352941177, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1787896, Length=400, Percent_Identity=41.25, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082375, Length=430, Percent_Identity=35.1162790697674, Blast_Score=247, Evalue=9e-67,
Organism=Escherichia coli, GI1790398, Length=322, Percent_Identity=27.639751552795, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17553882, Length=425, Percent_Identity=42.5882352941177, Blast_Score=320, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=40.4907975460123, Blast_Score=234, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324993, Length=406, Percent_Identity=41.871921182266, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6321806, Length=260, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24640177, Length=427, Percent_Identity=42.1545667447307, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24640179, Length=427, Percent_Identity=42.1545667447307, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI78710009, Length=429, Percent_Identity=41.025641025641, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24662684, Length=427, Percent_Identity=40.5152224824356, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24583245, Length=427, Percent_Identity=35.1288056206089, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 48719; Mature: 48719

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYVLLLMKYTDTAPKLFMNTGTRFPRRIIWAMGVLKKSCAKVNADLGLLDKKIANSIIK
CCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASEDLIDGKLDDKIVLDVFQTGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSN
HHHHHHCCCCCCHHEEEEHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
DTVPTAIRIAAVAEVTNKLLPALQQIISSLNKKAEEYKDVVKAGRTHLRDALPVTLGQEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SAYADAFQHEREQVMNILEYVKELPIGGTATGTGLNTHPEFQERVINEINRITGLGFKPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCH
NRFRAMRLLTDLLLLSGALRNIAVNLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCCHHHHCCCCC
MPGKTNPVTVEATLLISAQVVGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFVSEAL
CCCCCCCEEEEEEEEEEEHEECCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHH
EKMSKLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDKASEIGKKLNKGMSIREALRE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
LGYSDNEINKILDLSKLVKPGFTAK
CCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDYVLLLMKYTDTAPKLFMNTGTRFPRRIIWAMGVLKKSCAKVNADLGLLDKKIANSIIK
CCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASEDLIDGKLDDKIVLDVFQTGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSN
HHHHHHCCCCCCHHEEEEHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
DTVPTAIRIAAVAEVTNKLLPALQQIISSLNKKAEEYKDVVKAGRTHLRDALPVTLGQEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SAYADAFQHEREQVMNILEYVKELPIGGTATGTGLNTHPEFQERVINEINRITGLGFKPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCH
NRFRAMRLLTDLLLLSGALRNIAVNLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCCHHHHCCCCC
MPGKTNPVTVEATLLISAQVVGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFVSEAL
CCCCCCCEEEEEEEEEEEHEECCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHH
EKMSKLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDKASEIGKKLNKGMSIREALRE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
LGYSDNEINKILDLSKLVKPGFTAK
CCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8276121; 8119400; 11427726; 2124611 [H]