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The map label for this gene is gcvH3 [H]

Identifier: 227827403

GI number: 227827403

Start: 1063781

End: 1064203

Strand: Direct

Name: gcvH3 [H]

Synonym: M1425_1122

Alternate gene names: 227827403

Gene position: 1063781-1064203 (Clockwise)

Preceding gene: 227827399

Following gene: 227827404

Centisome position: 40.78

GC content: 34.52

Gene sequence:

>423_bases
ATGAAGATCTTAGGGTTTACCTTCCCTGATGATTTACTTTATGAACCGGAAAAACATGTGTGGGCAAGGATAGAAGATAA
TAATGTAATTAGTATAGGCGTTACTGATCTAGGACAATATATGGCTGGCAAAATATTTCAAGTTACAGCGAAACAGAAGG
GCGAGAAAGTAAATGGAAGGAGTGTTTTATTCTCCATTGAGAGCGCTAAATGGATAGGTAAGTTTAGGTTGCCAATAGAG
GGTGAAGTTTTTGACGTAAATGAAGAAGTAGTAAAAAATCCTTCATTAATTAATGAAAGACCTTATGATAGTTGGATAAT
TAAGATAAGGATAGAGGATGTCGATATTATAAAAAGAACTTTCAAACCAATTCAAGAAGCATACAAGCAATTTGAGGAGG
AGGCAAAAAGAGTTGTTAGATGA

Upstream 100 bases:

>100_bases
ATCTATTTTTAGAATAACTCCATCTAACCAAACTGCGAAATTAACCATAATTATGGTTTACTAAATAAAGTTTATAAGCT
TAAAAATATGTCAAATATTT

Downstream 100 bases:

>100_bases
TAAGGAAATAGTGTTAAGCGCATTAGAGAAGGTGGATAAGTTTTACGTTTACTTGGCTGGAATAAATAATAATGAAATAC
TTCTGGTTACTACGTTGAAC

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MKILGFTFPDDLLYEPEKHVWARIEDNNVISIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSLINERPYDSWIIKIRIEDVDIIKRTFKPIQEAYKQFEEEAKRVVR

Sequences:

>Translated_140_residues
MKILGFTFPDDLLYEPEKHVWARIEDNNVISIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSLINERPYDSWIIKIRIEDVDIIKRTFKPIQEAYKQFEEEAKRVVR
>Mature_140_residues
MKILGFTFPDDLLYEPEKHVWARIEDNNVISIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIE
GEVFDVNEEVVKNPSLINERPYDSWIIKIRIEDVDIIKRTFKPIQEAYKQFEEEAKRVVR

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=28.448275862069, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 16317; Mature: 16317

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILGFTFPDDLLYEPEKHVWARIEDNNVISIGVTDLGQYMAGKIFQVTAKQKGEKVNGR
CEEECCCCCHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCC
SVLFSIESAKWIGKFRLPIEGEVFDVNEEVVKNPSLINERPYDSWIIKIRIEDVDIIKRT
EEEEEECCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHH
FKPIQEAYKQFEEEAKRVVR
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILGFTFPDDLLYEPEKHVWARIEDNNVISIGVTDLGQYMAGKIFQVTAKQKGEKVNGR
CEEECCCCCHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCC
SVLFSIESAKWIGKFRLPIEGEVFDVNEEVVKNPSLINERPYDSWIIKIRIEDVDIIKRT
EEEEEECCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHH
FKPIQEAYKQFEEEAKRVVR
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]