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The map label for this gene is mcl2 [H]

Identifier: 227826824

GI number: 227826824

Start: 383512

End: 384321

Strand: Direct

Name: mcl2 [H]

Synonym: M1425_0440

Alternate gene names: 227826824

Gene position: 383512-384321 (Clockwise)

Preceding gene: 227826823

Following gene: 227826829

Centisome position: 14.7

GC content: 38.77

Gene sequence:

>810_bases
ATGATAAGGAGATCCCAGTTATACGTTCCCGCAATTTCCGAAAAGATGATAAGAAAATCGATTGAACTAAAGGCTGATTC
GATAATTTTCGATCTTGAAGATGCTGTACCTCCAGAGGATAAGGAAAGAGCAAGGGAATTGCTAATTAAACTTGTGAAGG
AATTGGATTGGGGTAAGAGGGAGTTATGCGTTAGAGTTAACTCTTTACAGCTACTTGATTCCTATAAGGATATTACAGGA
ATTTCAAAGGACGATAAGATCACGTGTATTGTTATACCTAAGGCTGAAAACGATCTCTCATTTCTCCACAAGGCAACTGG
GAAATACTTGATACCGTTAATAGAAACTGCAAAGGGTTTAGTTAATATTGAGGATATAGTTAGGTCTGAAGGTGTTGTCG
CTGTAAGTTATGGTGCTGCTGATCTTTCCTTATCCCTTGGTGGAGACTATAATTTCTATGAAAAGAACGAGTACGTGAAG
ACTTTAATTGTTAGTGTAGCTAGGGCTTACGATGTTGACGCCATAGATAAAGTTTACTTCGATTTGAAGAACCTAGATGG
ATTTAGAAGACAATGTGAGGAAGCAAAGAAGTTGGGGTACTTGGGAAAGCAAGTTATTCATCCTTCTCAGATTGACATTG
CCAATGAGGTATTTTCCCCAAGCAAGGAGGAAATCGAGTGGGCTAAAAGGGTTATAGAAGCCTACGAGAAGGGAAAAAGG
GAAGGTAAGGGTGCCATAAGGTTAGATGATAAATTAGTTGATTACGTGCATTATAAGATTGCTAAGAGGATAATTGAGTT
CCAGAGCTAG

Upstream 100 bases:

>100_bases
GATGAGAATGCATGAGGAGAAAGTTTTAGAATACGTGAAGCAAAACATTCTACCAGTGTTGTTTAGATAAAATTTTATGC
TCAATTCTCATTATTAACTT

Downstream 100 bases:

>100_bases
TATCCCTATATACAACCCAGGTACCTAAGGCCTTTGCGCATAAATTACCGTTACCATCATATGCTGATATGTCAACTACT
ATTAGCCTTTTTCCCTCACT

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIRRSQLYVPAISEKMIRKSIELKADSIIFDLEDAVPPEDKERARELLIKLVKELDWGKRELCVRVNSLQLLDSYKDITG
ISKDDKITCIVIPKAENDLSFLHKATGKYLIPLIETAKGLVNIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNEYVK
TLIVSVARAYDVDAIDKVYFDLKNLDGFRRQCEEAKKLGYLGKQVIHPSQIDIANEVFSPSKEEIEWAKRVIEAYEKGKR
EGKGAIRLDDKLVDYVHYKIAKRIIEFQS

Sequences:

>Translated_269_residues
MIRRSQLYVPAISEKMIRKSIELKADSIIFDLEDAVPPEDKERARELLIKLVKELDWGKRELCVRVNSLQLLDSYKDITG
ISKDDKITCIVIPKAENDLSFLHKATGKYLIPLIETAKGLVNIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNEYVK
TLIVSVARAYDVDAIDKVYFDLKNLDGFRRQCEEAKKLGYLGKQVIHPSQIDIANEVFSPSKEEIEWAKRVIEAYEKGKR
EGKGAIRLDDKLVDYVHYKIAKRIIEFQS
>Mature_269_residues
MIRRSQLYVPAISEKMIRKSIELKADSIIFDLEDAVPPEDKERARELLIKLVKELDWGKRELCVRVNSLQLLDSYKDITG
ISKDDKITCIVIPKAENDLSFLHKATGKYLIPLIETAKGLVNIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNEYVK
TLIVSVARAYDVDAIDKVYFDLKNLDGFRRQCEEAKKLGYLGKQVIHPSQIDIANEVFSPSKEEIEWAKRVIEAYEKGKR
EGKGAIRLDDKLVDYVHYKIAKRIIEFQS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=291, Percent_Identity=32.6460481099656, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI87081764, Length=269, Percent_Identity=30.4832713754647, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557458, Length=281, Percent_Identity=32.7402135231317, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30678; Mature: 30678

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRSQLYVPAISEKMIRKSIELKADSIIFDLEDAVPPEDKERARELLIKLVKELDWGKR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCH
ELCVRVNSLQLLDSYKDITGISKDDKITCIVIPKAENDLSFLHKATGKYLIPLIETAKGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEHHHHHHHCCC
VNIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNEYVKTLIVSVARAYDVDAIDKVYF
CCHHHHHHCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
DLKNLDGFRRQCEEAKKLGYLGKQVIHPSQIDIANEVFSPSKEEIEWAKRVIEAYEKGKR
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EGKGAIRLDDKLVDYVHYKIAKRIIEFQS
CCCCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRRSQLYVPAISEKMIRKSIELKADSIIFDLEDAVPPEDKERARELLIKLVKELDWGKR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCH
ELCVRVNSLQLLDSYKDITGISKDDKITCIVIPKAENDLSFLHKATGKYLIPLIETAKGL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEHHHHHHHCCC
VNIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNEYVKTLIVSVARAYDVDAIDKVYF
CCHHHHHHCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
DLKNLDGFRRQCEEAKKLGYLGKQVIHPSQIDIANEVFSPSKEEIEWAKRVIEAYEKGKR
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EGKGAIRLDDKLVDYVHYKIAKRIIEFQS
CCCCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA