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The map label for this gene is glmS [H]

Identifier: 227826814

GI number: 227826814

Start: 372937

End: 374691

Strand: Direct

Name: glmS [H]

Synonym: M1425_0428

Alternate gene names: 227826814

Gene position: 372937-374691 (Clockwise)

Preceding gene: 227826813

Following gene: 227826815

Centisome position: 14.3

GC content: 38.63

Gene sequence:

>1755_bases
ATGGGAGGAATTTTCGCTTTCGTATGCAAGGATTCCATTGATGTTTCAATTATTAACAAGGGTTTAAAGAAGTTGATTTA
TAGGGGATATGATAGCGCTGGTATTGCTTACCTTAAAGACGATAGCTTAGTAATAAAGAAAATTTTAGGTAATATCTCAA
AGAATGAGATAAGCGTTAGTGACAAGGCAAGAGTTGCAATAGGTCACACTAGATATGCGAGTAGGGGTTGGCCAACTTTG
GAAAACGCTCACCCACTGACTGATTGTAATGGGAAGATAGCAGTTGTAATGGACGGTATTCTTGACGATTACGAAAAAAT
TAGGGAAGATCTGATTGCGAAGGGACATAAATTCGTCTCTACAACTGACGCTGAGGTGATTCCCCACTTACTTGAGAATT
CAACAAACTATCTAAACTCATCATTAAACGTTATGAAAAGGGTAAAGGGCATTTACTCTCTGGTTTTTGTAACCATAGAC
ATTGATAAAATATTCGCAATTAACTCTGGCCAACCCTTGATGATAGGTACCACACAAGAGTGTAAATACGTTTCTAGCGA
TTTACCCTCTTTGAGCGGTTTTGCTGAGAATGCGATAATATTGCCAGAAAATACTGTGGCAGTAATCTCTTGGAATGATG
TGCAAGTGTATAATATTGAAGGTAATGAGGTAAAACCGGAAATTAAGAGAGTTAAATACAAGGAGGAGATAGCTGAAAAG
GGTGGATTTCCACACTTCATGTTAAAGGAGATATACGATATTCCACAGGCCTTGATAAACTCATTTAACTCTTTAATGGA
GAAGTACCTTTCCTTAGCCTCGATGATACTATATGGTGCTAAAAACGTTTATGTGATAGGTAATGGAACCAGTCTTCATG
CTGGATTAATCTCCACTTACTATTTCTCAGAAATCGGTCTAAATGTTAACGTTGTAAGTGCAGCGGAATTTCCATATTAT
GCCTTGGAAAACGTAACTACTGGTTCAGTAATTATTGCCATAAGTCAAAGTGGAGAGACCAGTGATGTTATAAGGAGTGT
TAAAATGGCCAAGCAGAGAGGAGCGGTGATATTAGGCATAACCAACTCCGTAGGTTCAAGATTGGCATTGGAGTCTAACG
TATACCTACCAATAACCGCTAGACCAGAAATGGCTGTGCCTGCAACCAAAACTTTTACCTCGACTATTGTGGTACTTAAG
GTACTTTCACTATATACTGGACTTCACTCCGGGAAAAATAGTAGGAGTGACATTAATTCATTAAAAACCGAGATCGAGGA
ATTGGCTAAACAACTCGTTATGAGTTTACCGGAAATGGAGAAAGAGGCAGAGAAATTGTCTATTAAACTGGATAAGGAAA
GCTTATACGTTTCAAGTAGTGGGATAAATTATCCCGTAGCATTGGAAGGAGCTTTGAAATTTAAGGAAGCTTCAATGACT
CACGCAGAGGGTATGCAGTTGGGAGAGCTCTTGCATGGCCCAATCGTTCTCACAAATAAAGGTTATCCCGTGATTTTAAT
AAAACCTGTTGAGGCTGAGGATTTGTACAATAAGGTTGTTAAGTCAATAAGGGAAAGGGGGGATGTGATTATAACCATTT
CTGAAGATGGTGATATGAAGAGTGTAAAGACAATTAGGGATTTGACTCCTATAAGCAATGTAATACCGTTACAGTTATTA
GCTTATAAGCTGGGAGTTAAGAGGGGTTTCCCAATAGACACTCCTCCGGGACTAGTAAAGGCTGTAATAGTTTAA

Upstream 100 bases:

>100_bases
CACCCAGAAGTGTATAGTCTGATTTTAGGTGTATTTACCTCGATTGAGCAGGGAAATGAGGGTTAGAGTTCCTTTTTATA
ACCAAAACTAAAATTTAAGT

Downstream 100 bases:

>100_bases
AAAGTAATAAATTACCCTTTTTCTATCTCTATTATATGACTATTGAAGTTGAGAACTTACAGAAGAAGTTTGACGATAAA
GCAGTGCTTAAGGGTGTTAG

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: D-fructose-6-phosphate amidotransferase; GFAT; Glucosamine-6-phosphate synthase; Hexosephosphate aminotransferase; L-glutamine-D-fructose-6-phosphate amidotransferase [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MGGIFAFVCKDSIDVSIINKGLKKLIYRGYDSAGIAYLKDDSLVIKKILGNISKNEISVSDKARVAIGHTRYASRGWPTL
ENAHPLTDCNGKIAVVMDGILDDYEKIREDLIAKGHKFVSTTDAEVIPHLLENSTNYLNSSLNVMKRVKGIYSLVFVTID
IDKIFAINSGQPLMIGTTQECKYVSSDLPSLSGFAENAIILPENTVAVISWNDVQVYNIEGNEVKPEIKRVKYKEEIAEK
GGFPHFMLKEIYDIPQALINSFNSLMEKYLSLASMILYGAKNVYVIGNGTSLHAGLISTYYFSEIGLNVNVVSAAEFPYY
ALENVTTGSVIIAISQSGETSDVIRSVKMAKQRGAVILGITNSVGSRLALESNVYLPITARPEMAVPATKTFTSTIVVLK
VLSLYTGLHSGKNSRSDINSLKTEIEELAKQLVMSLPEMEKEAEKLSIKLDKESLYVSSSGINYPVALEGALKFKEASMT
HAEGMQLGELLHGPIVLTNKGYPVILIKPVEAEDLYNKVVKSIRERGDVIITISEDGDMKSVKTIRDLTPISNVIPLQLL
AYKLGVKRGFPIDTPPGLVKAVIV

Sequences:

>Translated_584_residues
MGGIFAFVCKDSIDVSIINKGLKKLIYRGYDSAGIAYLKDDSLVIKKILGNISKNEISVSDKARVAIGHTRYASRGWPTL
ENAHPLTDCNGKIAVVMDGILDDYEKIREDLIAKGHKFVSTTDAEVIPHLLENSTNYLNSSLNVMKRVKGIYSLVFVTID
IDKIFAINSGQPLMIGTTQECKYVSSDLPSLSGFAENAIILPENTVAVISWNDVQVYNIEGNEVKPEIKRVKYKEEIAEK
GGFPHFMLKEIYDIPQALINSFNSLMEKYLSLASMILYGAKNVYVIGNGTSLHAGLISTYYFSEIGLNVNVVSAAEFPYY
ALENVTTGSVIIAISQSGETSDVIRSVKMAKQRGAVILGITNSVGSRLALESNVYLPITARPEMAVPATKTFTSTIVVLK
VLSLYTGLHSGKNSRSDINSLKTEIEELAKQLVMSLPEMEKEAEKLSIKLDKESLYVSSSGINYPVALEGALKFKEASMT
HAEGMQLGELLHGPIVLTNKGYPVILIKPVEAEDLYNKVVKSIRERGDVIITISEDGDMKSVKTIRDLTPISNVIPLQLL
AYKLGVKRGFPIDTPPGLVKAVIV
>Mature_583_residues
GGIFAFVCKDSIDVSIINKGLKKLIYRGYDSAGIAYLKDDSLVIKKILGNISKNEISVSDKARVAIGHTRYASRGWPTLE
NAHPLTDCNGKIAVVMDGILDDYEKIREDLIAKGHKFVSTTDAEVIPHLLENSTNYLNSSLNVMKRVKGIYSLVFVTIDI
DKIFAINSGQPLMIGTTQECKYVSSDLPSLSGFAENAIILPENTVAVISWNDVQVYNIEGNEVKPEIKRVKYKEEIAEKG
GFPHFMLKEIYDIPQALINSFNSLMEKYLSLASMILYGAKNVYVIGNGTSLHAGLISTYYFSEIGLNVNVVSAAEFPYYA
LENVTTGSVIIAISQSGETSDVIRSVKMAKQRGAVILGITNSVGSRLALESNVYLPITARPEMAVPATKTFTSTIVVLKV
LSLYTGLHSGKNSRSDINSLKTEIEELAKQLVMSLPEMEKEAEKLSIKLDKESLYVSSSGINYPVALEGALKFKEASMTH
AEGMQLGELLHGPIVLTNKGYPVILIKPVEAEDLYNKVVKSIRERGDVIITISEDGDMKSVKTIRDLTPISNVIPLQLLA
YKLGVKRGFPIDTPPGLVKAVIV

Specific function: Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source [H]

COG id: COG0449

COG function: function code M; Glucosamine 6-phosphate synthetase, contains amidotransferase and phosphosugar isomerase domains

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains [H]

Homologues:

Organism=Homo sapiens, GI4826742, Length=700, Percent_Identity=29, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI205277386, Length=692, Percent_Identity=27.7456647398844, Blast_Score=233, Evalue=5e-61,
Organism=Escherichia coli, GI1790167, Length=623, Percent_Identity=31.4606741573034, Blast_Score=268, Evalue=9e-73,
Organism=Escherichia coli, GI87082251, Length=300, Percent_Identity=20.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=442, Percent_Identity=29.6380090497738, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17532897, Length=442, Percent_Identity=29.6380090497738, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17539970, Length=440, Percent_Identity=27.9545454545455, Blast_Score=155, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322745, Length=438, Percent_Identity=31.2785388127854, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323731, Length=432, Percent_Identity=25.6944444444444, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323730, Length=205, Percent_Identity=31.7073170731707, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357745, Length=695, Percent_Identity=28.4892086330935, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR005855
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00310 GATase_2; PF01380 SIS [H]

EC number: =2.6.1.16 [H]

Molecular weight: Translated: 63955; Mature: 63824

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGIFAFVCKDSIDVSIINKGLKKLIYRGYDSAGIAYLKDDSLVIKKILGNISKNEISVS
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCEEECC
DKARVAIGHTRYASRGWPTLENAHPLTDCNGKIAVVMDGILDDYEKIREDLIAKGHKFVS
CCCEEEEECCEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEE
TTDAEVIPHLLENSTNYLNSSLNVMKRVKGIYSLVFVTIDIDKIFAINSGQPLMIGTTQE
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEHHEEEECCCCEEEEECCHH
CKYVSSDLPSLSGFAENAIILPENTVAVISWNDVQVYNIEGNEVKPEIKRVKYKEEIAEK
HHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHC
GGFPHFMLKEIYDIPQALINSFNSLMEKYLSLASMILYGAKNVYVIGNGTSLHAGLISTY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEHHHHHHHH
YFSEIGLNVNVVSAAEFPYYALENVTTGSVIIAISQSGETSDVIRSVKMAKQRGAVILGI
HHHHHCCEEEEEEECCCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEE
TNSVGSRLALESNVYLPITARPEMAVPATKTFTSTIVVLKVLSLYTGLHSGKNSRSDINS
CCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LKTEIEELAKQLVMSLPEMEKEAEKLSIKLDKESLYVSSSGINYPVALEGALKFKEASMT
HHHHHHHHHHHHHHHCHHHHHHHHHEEEEEECCEEEEECCCCCCCEEECCCHHHHHHHCC
HAEGMQLGELLHGPIVLTNKGYPVILIKPVEAEDLYNKVVKSIRERGDVIITISEDGDMK
HHCCHHHHHHHCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHH
SVKTIRDLTPISNVIPLQLLAYKLGVKRGFPIDTPPGLVKAVIV
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEC
>Mature Secondary Structure 
GGIFAFVCKDSIDVSIINKGLKKLIYRGYDSAGIAYLKDDSLVIKKILGNISKNEISVS
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCEEECC
DKARVAIGHTRYASRGWPTLENAHPLTDCNGKIAVVMDGILDDYEKIREDLIAKGHKFVS
CCCEEEEECCEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEE
TTDAEVIPHLLENSTNYLNSSLNVMKRVKGIYSLVFVTIDIDKIFAINSGQPLMIGTTQE
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEHHEEEECCCCEEEEECCHH
CKYVSSDLPSLSGFAENAIILPENTVAVISWNDVQVYNIEGNEVKPEIKRVKYKEEIAEK
HHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHC
GGFPHFMLKEIYDIPQALINSFNSLMEKYLSLASMILYGAKNVYVIGNGTSLHAGLISTY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEHHHHHHHH
YFSEIGLNVNVVSAAEFPYYALENVTTGSVIIAISQSGETSDVIRSVKMAKQRGAVILGI
HHHHHCCEEEEEEECCCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEE
TNSVGSRLALESNVYLPITARPEMAVPATKTFTSTIVVLKVLSLYTGLHSGKNSRSDINS
CCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LKTEIEELAKQLVMSLPEMEKEAEKLSIKLDKESLYVSSSGINYPVALEGALKFKEASMT
HHHHHHHHHHHHHHHCHHHHHHHHHEEEEEECCEEEEECCCCCCCEEECCCHHHHHHHCC
HAEGMQLGELLHGPIVLTNKGYPVILIKPVEAEDLYNKVVKSIRERGDVIITISEDGDMK
HHCCHHHHHHHCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHH
SVKTIRDLTPISNVIPLQLLAYKLGVKRGFPIDTPPGLVKAVIV
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]