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The map label for this gene is alaXS [H]

Identifier: 227826811

GI number: 227826811

Start: 370580

End: 371032

Strand: Direct

Name: alaXS [H]

Synonym: M1425_0425

Alternate gene names: 227826811

Gene position: 370580-371032 (Clockwise)

Preceding gene: 227826802

Following gene: 227826812

Centisome position: 14.2

GC content: 39.29

Gene sequence:

>453_bases
ATGGATCAAGTTGAGATTCGCACTCACACCGCCTTACATGTGGTCAAAGGAGCTGTGAGGAAAGTTTTGGGTGCCAAATG
GACTGCAAGCACTTACGTTAATAGCAATCATGGAAGATTAACGGTAAAGTTTGAGAGGAAACCTAGCGATGAGGAAATGG
ATAAGGTCTTTGAATTGGCTAACGAGAAGGTTAGAAAAAACCTTCCAATAATAATTGAGGTTTTACCTAGGGAAGAAGCT
GAAAGGAAATATGGAGATGAGATCTACGATCTCTTTCTTATTCCAGCTGAGGTTAGGGAGTTGAGCATTGTGGTAATACC
AGATTGGAATATTAACGCTTGCAATAAGCAACATACTAAGACTACCTCTGAGATTGGAGAGATCATCAAGGACTATTGGA
GATATAGGAATAGTAAACAGTTACTCGAGATGTCATTTGACATAAAGTGTTAA

Upstream 100 bases:

>100_bases
ATTTGCTCATCTTTTATCTCCTTCCAACCTGGGAAGATTGAGAAACCAATTCTTCTCTTAGTCATACTATATAAAAGGCT
TTGAGGATAATATATTTGAC

Downstream 100 bases:

>100_bases
GAATGATAGGAAAAGTCCTTGAGCTTTTAAACTGTAAGGAGACTGAGAGGGAAATTAACAGAAGAAAATTTTCAGTATGC
GATGATGTTAACTTCATCTT

Product: alanyl-tRNA synthetase-like protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-S; Alanyl-tRNA deacylase AlaX-S; PhoAlaX [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDEEMDKVFELANEKVRKNLPIIIEVLPREEA
ERKYGDEIYDLFLIPAEVRELSIVVIPDWNINACNKQHTKTTSEIGEIIKDYWRYRNSKQLLEMSFDIKC

Sequences:

>Translated_150_residues
MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDEEMDKVFELANEKVRKNLPIIIEVLPREEA
ERKYGDEIYDLFLIPAEVRELSIVVIPDWNINACNKQHTKTTSEIGEIIKDYWRYRNSKQLLEMSFDIKC
>Mature_150_residues
MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDEEMDKVFELANEKVRKNLPIIIEVLPREEA
ERKYGDEIYDLFLIPAEVRELSIVVIPDWNINACNKQHTKTTSEIGEIIKDYWRYRNSKQLLEMSFDIKC

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged Ser-tRNA(Ala). Has little activity against Gly- tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-S subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 17532; Mature: 17532

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDEEMDKVFELA
CCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NEKVRKNLPIIIEVLPREEAERKYGDEIYDLFLIPAEVRELSIVVIPDWNINACNKQHTK
HHHHHCCCCEEEEECCCCHHHHHCCCHHHHHEECCCCCCEEEEEEEECCCCCCCCCCCCC
TTSEIGEIIKDYWRYRNSKQLLEMSFDIKC
CHHHHHHHHHHHHHCCCCHHHHHEECCCCC
>Mature Secondary Structure
MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDEEMDKVFELA
CCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NEKVRKNLPIIIEVLPREEAERKYGDEIYDLFLIPAEVRELSIVVIPDWNINACNKQHTK
HHHHHCCCCEEEEECCCCHHHHHCCCHHHHHEECCCCCCEEEEEEEECCCCCCCCCCCCC
TTSEIGEIIKDYWRYRNSKQLLEMSFDIKC
CHHHHHHHHHHHHHCCCCHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]