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The map label for this gene is dac [H]

Identifier: 227826795

GI number: 227826795

Start: 352407

End: 352967

Strand: Direct

Name: dac [H]

Synonym: M1425_0404

Alternate gene names: 227826795

Gene position: 352407-352967 (Clockwise)

Preceding gene: 227826794

Following gene: 227826796

Centisome position: 13.51

GC content: 37.43

Gene sequence:

>561_bases
ATGCAAGTTTTGATCGTTGCGCCCCATCCAGATGATGAGACTTTGTGTTGTGGTGGGGTTGTTCAGATTTTTAAAGAAAG
GGGTTATAAGGTAAGTGTCGTTGTAGTAACTGACGGAAGATACGGTTCTCCAGACGAGAAATTAAAAGGAAGTATGGAGT
TGGTTGAGATTAGGAGACAAGAGGCATTAAGGGCTAGTAAGATTTTAGGTATAGATGAGATTACATTCTTGAGTTTCGAG
GACTCTAAGGTTATTGAAAAGGAAGTTGAAAAAGCGTTAGTAGAGTTTTTACATGATAAAGATATAGTCCTTTCTCCGAT
ACCCTTCGATAGTCATCCAGATCACGCTAAAATAGGTAAAGTGATGGAGAAGTTATATCCAAAGGCTTACTTTTACTTAA
TATGGGGTTCTATGCAAGCTAATTGGAGAGAGGTTAGGTTTGATATTAGAAAGTATAGAGAGGTCAAGATTAGGGCTATA
AATGAGTATAAAAGTCAGATTGGTGGTCTTCATTTGGATAGATTCACTGGAGACTATGAGGTTTTTTGGATGAAGGTTTA
A

Upstream 100 bases:

>100_bases
AGCGAAGAAGTAGTACCAATTCCAGAGGAATATTCCTCTTGGGATAAGGTTGTTGAAGCAGAATTGGAAGATATAAAAGC
CTTTGCGAAGAGATAATCCC

Downstream 100 bases:

>100_bases
ATTGTGGAATTCGAGGTTTTATATATTGCAGATGACGTATTGTATAGGACTTTGGGAGAATTATTAGGCAATTACGTTAA
AACATTACTAGAGAAGAGTG

Product: LmbE family protein

Products: NA

Alternate protein names: N-acetylchitobiose deacetylase; Tk-Dac [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MQVLIVAPHPDDETLCCGGVVQIFKERGYKVSVVVVTDGRYGSPDEKLKGSMELVEIRRQEALRASKILGIDEITFLSFE
DSKVIEKEVEKALVEFLHDKDIVLSPIPFDSHPDHAKIGKVMEKLYPKAYFYLIWGSMQANWREVRFDIRKYREVKIRAI
NEYKSQIGGLHLDRFTGDYEVFWMKV

Sequences:

>Translated_186_residues
MQVLIVAPHPDDETLCCGGVVQIFKERGYKVSVVVVTDGRYGSPDEKLKGSMELVEIRRQEALRASKILGIDEITFLSFE
DSKVIEKEVEKALVEFLHDKDIVLSPIPFDSHPDHAKIGKVMEKLYPKAYFYLIWGSMQANWREVRFDIRKYREVKIRAI
NEYKSQIGGLHLDRFTGDYEVFWMKV
>Mature_186_residues
MQVLIVAPHPDDETLCCGGVVQIFKERGYKVSVVVVTDGRYGSPDEKLKGSMELVEIRRQEALRASKILGIDEITFLSFE
DSKVIEKEVEKALVEFLHDKDIVLSPIPFDSHPDHAKIGKVMEKLYPKAYFYLIWGSMQANWREVRFDIRKYREVKIRAI
NEYKSQIGGLHLDRFTGDYEVFWMKV

Specific function: Deacylates the non-reducing end of diacetylchitobiose (GlcNAc2). Can also use N-acetylglucosamine (GlcNAc) and N- acetylchitotriose (GlcNAc3). Probably involved in chitin degradation [H]

COG id: COG2120

COG function: function code S; Uncharacterized proteins, LmbE homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003737 [H]

Pfam domain/function: PF02585 PIG-L [H]

EC number: NA

Molecular weight: Translated: 21585; Mature: 21585

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLIVAPHPDDETLCCGGVVQIFKERGYKVSVVVVTDGRYGSPDEKLKGSMELVEIRRQ
CEEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCHHHHHHHHH
EALRASKILGIDEITFLSFEDSKVIEKEVEKALVEFLHDKDIVLSPIPFDSHPDHAKIGK
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
VMEKLYPKAYFYLIWGSMQANWREVRFDIRKYREVKIRAINEYKSQIGGLHLDRFTGDYE
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHCCEEEEEECCCEE
VFWMKV
EEEEEC
>Mature Secondary Structure
MQVLIVAPHPDDETLCCGGVVQIFKERGYKVSVVVVTDGRYGSPDEKLKGSMELVEIRRQ
CEEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCHHHHHHHHH
EALRASKILGIDEITFLSFEDSKVIEKEVEKALVEFLHDKDIVLSPIPFDSHPDHAKIGK
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
VMEKLYPKAYFYLIWGSMQANWREVRFDIRKYREVKIRAINEYKSQIGGLHLDRFTGDYE
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHCCEEEEEECCCEE
VFWMKV
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA