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The map label for this gene is glcD [H]

Identifier: 227826777

GI number: 227826777

Start: 330015

End: 331358

Strand: Direct

Name: glcD [H]

Synonym: M1425_0380

Alternate gene names: 227826777

Gene position: 330015-331358 (Clockwise)

Preceding gene: 227826776

Following gene: 227826780

Centisome position: 12.65

GC content: 38.32

Gene sequence:

>1344_bases
ATGGATTTCACCGAATTGAAAGATATCGAGCAAAAGGTAGAGGAAAGGGAGGATTTTTCCGGAGAGAAAGTAAGACCATT
AGCCGTATTCTTCCCTAAGGATGAGGATGAAGTCGTTAGGATTGTTAGATTTGCTAAGAAAAATAGATTACCCATAATAC
CATGGGGTCAAGGAACTAGTTTAACCGGAGCTGTTTCGTGCGATAAAAACTGTATATTAGTAGACCTATCTAAAATGAAT
AAAATTTTAGAGATAAACGATATTGATTGGTACGTAAGAGTCCAACCGGGGATTAAATTAATAGACCTCTTCGAGGAACT
AGAGAAAAAAGGCTTTATGTTACCTCCAGATCCAGCAAGCTTCTTTTTATGCTCAGTTGGTGGGGCAGTAGCTGAATCCT
CTGGAGGGATGAAGGGTGTAAGGCACGGTTCATTCAGGGAATGGGTATTATCTTTACGTGTTGTTTTACCAAATGGGGAG
GTAATTAAGGTCGGAGAACCATTGAGGAAGAATAGGGCCGGATATGATTTGGTTCATTTATTCGTTGGAAGTGAGGGAAC
TTTAGGTATCGTAACGGAAATCTGGTTGAGGATAATACCAATTCCAAAGAGAAAGATGGTTATGATAGCTGCAATGTTAA
AGGATTTTGAATCCGCTGGAGAAGTTATAGTAGGCTTGAGGAAGAATAAGATATTGCCAGAGCTGTCAGAGTACGTTGAT
GCTGATGTAGTCAAAGCGTTAAATAAACACTTGAGTGCAAACCTCAAGGAAACAGAGGGAGGGATGTTATTAATTTCAAT
AGAGGAAGATAGTGTCGATGATGTGTTAAAAGTATTAGAGGGAAAGGCAGTTGATATTAAGATTGCTGAAGGTGAAGAGG
CAGAGAAATTGTATTCATTGAGATCACAAGCTGCAATAGCAGTGAAAGCTGAATCAAATAAAGTATTTTACGCTGAAGAT
ATAGTAGTACCAGTTTCTAAACTGCCAGAAGCTATAAGGAGACTCAGAGAAATAGGTGAGAAGTATAATACTAAATTCTA
CGTTATCTCGCATATAGGTGATGGTAATCTTCATCCAAACATAATAATTGAGGATAAGGTAGCAAGGGAAAATGCATTTG
AGGAAATAGCTAGAATGGCGATTGAGCTTGGGGGTTCTGTTAGTGGAGAGCATGGAATTGGTGTGCAGAAGGCTAAGTTA
ATGGCTGAGCAAATAGTTAAACATAATGGCGTTAGTGTATTGGATTTAATGTATCAGATAAAGAAGTTAATTGATCCAGA
TGATATAATGAACCCTGACAAGTATGTCGAATTGGCATATAAGTTTTTAACTTCTAGGGGGTAG

Upstream 100 bases:

>100_bases
CTTTTTAATTTTAAGGAATAAATTTGAGCATTATATAAGGGACAAAGCTATTCATTTGCCCTAAAAAATCCTTTTATTAA
CTCACGTATAATTGTTTATT

Downstream 100 bases:

>100_bases
AAGTGCTATTTGATCCTTACCCAAAAACTGAAAGTAGAGATTTCTTCCATAGGGAAAACGAAATTCCTTAACGTTAAAAT
TCCGCATTAACTTTCTTCTT

Product: FAD linked oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDFTELKDIEQKVEEREDFSGEKVRPLAVFFPKDEDEVVRIVRFAKKNRLPIIPWGQGTSLTGAVSCDKNCILVDLSKMN
KILEINDIDWYVRVQPGIKLIDLFEELEKKGFMLPPDPASFFLCSVGGAVAESSGGMKGVRHGSFREWVLSLRVVLPNGE
VIKVGEPLRKNRAGYDLVHLFVGSEGTLGIVTEIWLRIIPIPKRKMVMIAAMLKDFESAGEVIVGLRKNKILPELSEYVD
ADVVKALNKHLSANLKETEGGMLLISIEEDSVDDVLKVLEGKAVDIKIAEGEEAEKLYSLRSQAAIAVKAESNKVFYAED
IVVPVSKLPEAIRRLREIGEKYNTKFYVISHIGDGNLHPNIIIEDKVARENAFEEIARMAIELGGSVSGEHGIGVQKAKL
MAEQIVKHNGVSVLDLMYQIKKLIDPDDIMNPDKYVELAYKFLTSRG

Sequences:

>Translated_447_residues
MDFTELKDIEQKVEEREDFSGEKVRPLAVFFPKDEDEVVRIVRFAKKNRLPIIPWGQGTSLTGAVSCDKNCILVDLSKMN
KILEINDIDWYVRVQPGIKLIDLFEELEKKGFMLPPDPASFFLCSVGGAVAESSGGMKGVRHGSFREWVLSLRVVLPNGE
VIKVGEPLRKNRAGYDLVHLFVGSEGTLGIVTEIWLRIIPIPKRKMVMIAAMLKDFESAGEVIVGLRKNKILPELSEYVD
ADVVKALNKHLSANLKETEGGMLLISIEEDSVDDVLKVLEGKAVDIKIAEGEEAEKLYSLRSQAAIAVKAESNKVFYAED
IVVPVSKLPEAIRRLREIGEKYNTKFYVISHIGDGNLHPNIIIEDKVARENAFEEIARMAIELGGSVSGEHGIGVQKAKL
MAEQIVKHNGVSVLDLMYQIKKLIDPDDIMNPDKYVELAYKFLTSRG
>Mature_447_residues
MDFTELKDIEQKVEEREDFSGEKVRPLAVFFPKDEDEVVRIVRFAKKNRLPIIPWGQGTSLTGAVSCDKNCILVDLSKMN
KILEINDIDWYVRVQPGIKLIDLFEELEKKGFMLPPDPASFFLCSVGGAVAESSGGMKGVRHGSFREWVLSLRVVLPNGE
VIKVGEPLRKNRAGYDLVHLFVGSEGTLGIVTEIWLRIIPIPKRKMVMIAAMLKDFESAGEVIVGLRKNKILPELSEYVD
ADVVKALNKHLSANLKETEGGMLLISIEEDSVDDVLKVLEGKAVDIKIAEGEEAEKLYSLRSQAAIAVKAESNKVFYAED
IVVPVSKLPEAIRRLREIGEKYNTKFYVISHIGDGNLHPNIIIEDKVARENAFEEIARMAIELGGSVSGEHGIGVQKAKL
MAEQIVKHNGVSVLDLMYQIKKLIDPDDIMNPDKYVELAYKFLTSRG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=32.4705882352941, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=30.8035714285714, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=29.5612009237875, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4501993, Length=212, Percent_Identity=32.0754716981132, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1789351, Length=441, Percent_Identity=29.9319727891156, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787977, Length=515, Percent_Identity=24.8543689320388, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994907, Length=218, Percent_Identity=25.2293577981651, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI48994914, Length=136, Percent_Identity=31.6176470588235, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=429, Percent_Identity=30.0699300699301, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=427, Percent_Identity=28.8056206088993, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17534361, Length=449, Percent_Identity=28.7305122494432, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71992392, Length=366, Percent_Identity=31.6939890710383, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=462, Percent_Identity=26.4069264069264, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320027, Length=432, Percent_Identity=31.0185185185185, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6320023, Length=434, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320764, Length=444, Percent_Identity=28.6036036036036, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18921117, Length=429, Percent_Identity=26.3403263403263, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24639277, Length=429, Percent_Identity=26.3403263403263, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24639275, Length=429, Percent_Identity=26.3403263403263, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24653753, Length=457, Percent_Identity=27.1334792122538, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49888; Mature: 49888

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTELKDIEQKVEEREDFSGEKVRPLAVFFPKDEDEVVRIVRFAKKNRLPIIPWGQGTS
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCC
LTGAVSCDKNCILVDLSKMNKILEINDIDWYVRVQPGIKLIDLFEELEKKGFMLPPDPAS
EEEEEECCCCEEEEEHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHH
FFLCSVGGAVAESSGGMKGVRHGSFREWVLSLRVVLPNGEVIKVGEPLRKNRAGYDLVHL
EEEECCCCHHHCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEECCCCHHHCCCCCEEEEE
FVGSEGTLGIVTEIWLRIIPIPKRKMVMIAAMLKDFESAGEVIVGLRKNKILPELSEYVD
EECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
ADVVKALNKHLSANLKETEGGMLLISIEEDSVDDVLKVLEGKAVDIKIAEGEEAEKLYSL
HHHHHHHHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHH
RSQAAIAVKAESNKVFYAEDIVVPVSKLPEAIRRLREIGEKYNTKFYVISHIGDGNLHPN
HHCCEEEEEECCCEEEEECCEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
IIIEDKVARENAFEEIARMAIELGGSVSGEHGIGVQKAKLMAEQIVKHNGVSVLDLMYQI
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KKLIDPDDIMNPDKYVELAYKFLTSRG
HHHCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFTELKDIEQKVEEREDFSGEKVRPLAVFFPKDEDEVVRIVRFAKKNRLPIIPWGQGTS
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCC
LTGAVSCDKNCILVDLSKMNKILEINDIDWYVRVQPGIKLIDLFEELEKKGFMLPPDPAS
EEEEEECCCCEEEEEHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHH
FFLCSVGGAVAESSGGMKGVRHGSFREWVLSLRVVLPNGEVIKVGEPLRKNRAGYDLVHL
EEEECCCCHHHCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEECCCCHHHCCCCCEEEEE
FVGSEGTLGIVTEIWLRIIPIPKRKMVMIAAMLKDFESAGEVIVGLRKNKILPELSEYVD
EECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
ADVVKALNKHLSANLKETEGGMLLISIEEDSVDDVLKVLEGKAVDIKIAEGEEAEKLYSL
HHHHHHHHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHH
RSQAAIAVKAESNKVFYAEDIVVPVSKLPEAIRRLREIGEKYNTKFYVISHIGDGNLHPN
HHCCEEEEEECCCEEEEECCEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
IIIEDKVARENAFEEIARMAIELGGSVSGEHGIGVQKAKLMAEQIVKHNGVSVLDLMYQI
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KKLIDPDDIMNPDKYVELAYKFLTSRG
HHHCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]