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The map label for this gene is thcD [H]

Identifier: 227826735

GI number: 227826735

Start: 290797

End: 292020

Strand: Direct

Name: thcD [H]

Synonym: M1425_0334

Alternate gene names: 227826735

Gene position: 290797-292020 (Clockwise)

Preceding gene: 227826733

Following gene: 227826736

Centisome position: 11.15

GC content: 39.62

Gene sequence:

>1224_bases
ATGCGCGAAAAATCTTGTGACTACTTGATAATTGGGAGTGGTATTGCGGGATATAATGCATTAAAGGAACTACTAGAACT
AAAACCAAACTCAAAAATAATCATGGTCTCATCAGACAAATATTACCCATATGATAGACCACCACTATCAAAATACTATC
TTAGAGGAGAAATGGCACGTGAGAAACTATTCTTTGAGTCTGATGATTTTTACAAGAGGAGTAATTTGGATGTTGTTTTG
AATAGGAATGTTGAGAGGATTGATACTATTTCTAAGGAGGCAATATTGAATGATGGTAGTGTGATATCCTTTGATAGGGC
CTTGATAGCTAGTGGTGGTAGGCCTAGGAGGTTGAGTGTTCCGGGAGGTGAGAATGCACTATATTTGAGGACATTGGATG
ATTGTGATGGAATAAGGGAGGTTGCGAGTAGGAGTAGGAATGCGTTAATAATTGGTGCTGGTTTCATAGGTGTTGAGGTA
GCGTCAAGTCTAACAACGCTTGGTGTGAGGACTACAGTAGTGGAAGTAATGCCGTACATATGGAACACTTTTGTGGATGA
AAAGGTATCTAGGGTTATACGGCAATACTTGGAGGGTAAGGGGATAAACTTCATATTGAACGAGTCAGTAAGGGAGATTC
AAGGAAAAGCTGCGATAACGTCTGGTGGTAAGAGATTGGAAGCGGATATGTTTCTGATCGCTGTGGGAATTGTACCAAAT
GTTGAGGTAGCGCAAAAGAGTGGGATACAAGTTGATAACGGTGTAATTGTGAATGAATACTTGGAGACGAGTGCTAAGGA
CATTTATGCGGCTGGGGATGTTGCAAGTATATTTGATCCTAGGGAGGGTAGGAGGAAGAGGATTGAGCACTGGAATAACG
CTGAGTACACTGGAAAATTGGCAGCTAGGAACATGGCTGGTAATAGAGAAGCCTACAATTTCATATCATCCATATGGTCG
GATATATTCGATATTCACATAGAATCTGCTGGTGAGACGAGGAATTATGACGAATACGTAATAAGAGGAAGATTCAATTC
TGATAGTCCTAATTTTAATGTAATATATTTGAAAGGTGGTATAGTTAAGGGGTATGTTGCGATAAATAGGGATTATGGCG
AATTAGAGGTTCTAAATAAGCTGATTAAAGAAGGTAAAGATGTTTCAAATAAGAAGAACTCTCTGGCAGATGAGGGATTC
GACCTTAAGGAGTTACTGAAATGA

Upstream 100 bases:

>100_bases
CCTATAAAACGGAATTAGAGTTAAGAAAGCGTAATTATAAGGAAAAATATAAACCATCTTGAATTTAAGGTTTATAATAA
TCCTCAACAAGTTTATTATT

Downstream 100 bases:

>100_bases
CCCTTTTTTCTTTCGGATAAGTAGTTTAGTGGTTAAAAGTTAAACTTTTTACTTAACTATCTAACTTCTCCCCATGTCAT
CCAGTACACCATTTCTCTTT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MREKSCDYLIIGSGIAGYNALKELLELKPNSKIIMVSSDKYYPYDRPPLSKYYLRGEMAREKLFFESDDFYKRSNLDVVL
NRNVERIDTISKEAILNDGSVISFDRALIASGGRPRRLSVPGGENALYLRTLDDCDGIREVASRSRNALIIGAGFIGVEV
ASSLTTLGVRTTVVEVMPYIWNTFVDEKVSRVIRQYLEGKGINFILNESVREIQGKAAITSGGKRLEADMFLIAVGIVPN
VEVAQKSGIQVDNGVIVNEYLETSAKDIYAAGDVASIFDPREGRRKRIEHWNNAEYTGKLAARNMAGNREAYNFISSIWS
DIFDIHIESAGETRNYDEYVIRGRFNSDSPNFNVIYLKGGIVKGYVAINRDYGELEVLNKLIKEGKDVSNKKNSLADEGF
DLKELLK

Sequences:

>Translated_407_residues
MREKSCDYLIIGSGIAGYNALKELLELKPNSKIIMVSSDKYYPYDRPPLSKYYLRGEMAREKLFFESDDFYKRSNLDVVL
NRNVERIDTISKEAILNDGSVISFDRALIASGGRPRRLSVPGGENALYLRTLDDCDGIREVASRSRNALIIGAGFIGVEV
ASSLTTLGVRTTVVEVMPYIWNTFVDEKVSRVIRQYLEGKGINFILNESVREIQGKAAITSGGKRLEADMFLIAVGIVPN
VEVAQKSGIQVDNGVIVNEYLETSAKDIYAAGDVASIFDPREGRRKRIEHWNNAEYTGKLAARNMAGNREAYNFISSIWS
DIFDIHIESAGETRNYDEYVIRGRFNSDSPNFNVIYLKGGIVKGYVAINRDYGELEVLNKLIKEGKDVSNKKNSLADEGF
DLKELLK
>Mature_407_residues
MREKSCDYLIIGSGIAGYNALKELLELKPNSKIIMVSSDKYYPYDRPPLSKYYLRGEMAREKLFFESDDFYKRSNLDVVL
NRNVERIDTISKEAILNDGSVISFDRALIASGGRPRRLSVPGGENALYLRTLDDCDGIREVASRSRNALIIGAGFIGVEV
ASSLTTLGVRTTVVEVMPYIWNTFVDEKVSRVIRQYLEGKGINFILNESVREIQGKAAITSGGKRLEADMFLIAVGIVPN
VEVAQKSGIQVDNGVIVNEYLETSAKDIYAAGDVASIFDPREGRRKRIEHWNNAEYTGKLAARNMAGNREAYNFISSIWS
DIFDIHIESAGETRNYDEYVIRGRFNSDSPNFNVIYLKGGIVKGYVAINRDYGELEVLNKLIKEGKDVSNKKNSLADEGF
DLKELLK

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=377, Percent_Identity=29.1777188328912, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI226437568, Length=377, Percent_Identity=29.1777188328912, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI65787454, Length=377, Percent_Identity=29.1777188328912, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4757732, Length=344, Percent_Identity=30.5232558139535, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI22202629, Length=344, Percent_Identity=30.5232558139535, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI22202631, Length=198, Percent_Identity=32.3232323232323, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI195927004, Length=103, Percent_Identity=39.8058252427184, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI195927006, Length=165, Percent_Identity=29.6969696969697, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI50301238, Length=204, Percent_Identity=30.3921568627451, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788892, Length=410, Percent_Identity=28.2926829268293, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789065, Length=347, Percent_Identity=23.9193083573487, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789765, Length=279, Percent_Identity=27.2401433691756, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786307, Length=231, Percent_Identity=29.4372294372294, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082354, Length=276, Percent_Identity=27.1739130434783, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=337, Percent_Identity=27.893175074184, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564386, Length=442, Percent_Identity=26.4705882352941, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32565766, Length=257, Percent_Identity=30.3501945525292, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573993, Length=367, Percent_Identity=32.6975476839237, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24581020, Length=367, Percent_Identity=32.6975476839237, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24639257, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281359715, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281359713, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639250, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18543267, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639252, Length=349, Percent_Identity=27.5071633237822, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585130, Length=338, Percent_Identity=23.6686390532544, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 45611; Mature: 45611

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKSCDYLIIGSGIAGYNALKELLELKPNSKIIMVSSDKYYPYDRPPLSKYYLRGEMAR
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EKLFFESDDFYKRSNLDVVLNRNVERIDTISKEAILNDGSVISFDRALIASGGRPRRLSV
HHHCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCEEEC
PGGENALYLRTLDDCDGIREVASRSRNALIIGAGFIGVEVASSLTTLGVRTTVVEVMPYI
CCCCCEEEEEECHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
WNTFVDEKVSRVIRQYLEGKGINFILNESVREIQGKAAITSGGKRLEADMFLIAVGIVPN
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCCEEECCCCCEEECCEEEEEEECCCC
VEVAQKSGIQVDNGVIVNEYLETSAKDIYAAGDVASIFDPREGRRKRIEHWNNAEYTGKL
CHHHHHCCCEECCCEEEHHHHHCCHHHEEECCCHHHHCCCCCHHHHHHHCCCCCCEEHHH
AARNMAGNREAYNFISSIWSDIFDIHIESAGETRNYDEYVIRGRFNSDSPNFNVIYLKGG
HHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECC
IVKGYVAINRDYGELEVLNKLIKEGKDVSNKKNSLADEGFDLKELLK
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MREKSCDYLIIGSGIAGYNALKELLELKPNSKIIMVSSDKYYPYDRPPLSKYYLRGEMAR
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EKLFFESDDFYKRSNLDVVLNRNVERIDTISKEAILNDGSVISFDRALIASGGRPRRLSV
HHHCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCEEEC
PGGENALYLRTLDDCDGIREVASRSRNALIIGAGFIGVEVASSLTTLGVRTTVVEVMPYI
CCCCCEEEEEECHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
WNTFVDEKVSRVIRQYLEGKGINFILNESVREIQGKAAITSGGKRLEADMFLIAVGIVPN
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCCEEECCCCCEEECCEEEEEEECCCC
VEVAQKSGIQVDNGVIVNEYLETSAKDIYAAGDVASIFDPREGRRKRIEHWNNAEYTGKL
CHHHHHCCCEECCCEEEHHHHHCCHHHEEECCCHHHHCCCCCHHHHHHHCCCCCCEEHHH
AARNMAGNREAYNFISSIWSDIFDIHIESAGETRNYDEYVIRGRFNSDSPNFNVIYLKGG
HHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECC
IVKGYVAINRDYGELEVLNKLIKEGKDVSNKKNSLADEGFDLKELLK
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]