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The map label for this gene is rutB [H]

Identifier: 227826653

GI number: 227826653

Start: 210021

End: 210638

Strand: Direct

Name: rutB [H]

Synonym: M1425_0244

Alternate gene names: 227826653

Gene position: 210021-210638 (Clockwise)

Preceding gene: 227826652

Following gene: 227826654

Centisome position: 8.05

GC content: 34.79

Gene sequence:

>618_bases
GTGCGTGCAGTAAATTTAATTATGAAAATTGAAGTACCATCTATTCCCGAGCATAAAGAGGTAATATTAGATCCTACTAA
TACAGCATTAATAATTGTAGACATGCAAAACGACTTTGTGAGGAAAAACGGTAAATTATCAGTTCCTACTGCAGAGGCTA
CTATACCATTTATAAAGAGATTAGTCGATAAGGCAAGAAGTTCCAATGCGTTGGTAATATATACACAAGATTGGCACATG
AAAGATGACCCAGAATTTAAAATATGGGGAGAGCACGCATTGGCTGGAACTTGGGGTGCAGAAATAATTGACGAATTGAC
TCCAGAAAAGAGCGATTTCATAGTTAAGAAGTATAGATATGATGCCTTCTTTGAATCCTCATTAGACTATATTCTTAGAG
TTAAGAATATCAAAAATACCATAATTACTGGGACTGTCGCAAATATTTGCGTATTACACACTGCTGGTAGTGCTGCTTTA
AGATGGTATAATGTAATTATGCCAAAGGATTCAATATCTGCAATAACGGAGTTTGATTATTATGCTACTTTAAGGCAAGT
AGACTTTTTATACAAGGGTATAATAACTACTGCAGATGGCATTAAATTTGAGAGGTAG

Upstream 100 bases:

>100_bases
GTCGACTTACAAGATTGCATTGAACTAGGTAAGAGGGGTTATCCTAAAGTTCTAAAAATAGCTGATATGCTCACTAAGAA
GATAAAAAAGGAATAAAACT

Downstream 100 bases:

>100_bases
GTAAAAAGTTGGAATATTATGCAATAGTACATAACGATTTTGATGGAACAGCATCTGCAGCCGTTTACGCTAGGGCAATT
AAATCATTACCTAAAAATGT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER

Sequences:

>Translated_205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER
>Mature_205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=202, Percent_Identity=27.7227722772277, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786811, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081970, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 23361; Mature: 23361

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKR
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCCEECCCCHHHHHHHHH
LVDKARSSNALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRY
HHHHHCCCCEEEEEEECCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEHHH
DAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDY
HHHHHCCHHEEEEEECCCCEEEECCHHEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHH
YATLRQVDFLYKGIITTADGIKFER
HHHHHHHHHHHHHHEECCCCEEECC
>Mature Secondary Structure
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKR
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCCEECCCCHHHHHHHHH
LVDKARSSNALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRY
HHHHHCCCCEEEEEEECCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEHHH
DAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDY
HHHHHCCHHEEEEEECCCCEEEECCHHEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHH
YATLRQVDFLYKGIITTADGIKFER
HHHHHHHHHHHHHHEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA