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The map label for this gene is hofB [C]

Identifier: 227826591

GI number: 227826591

Start: 158719

End: 160452

Strand: Direct

Name: hofB [C]

Synonym: M1425_0181

Alternate gene names: 227826591

Gene position: 158719-160452 (Clockwise)

Preceding gene: 227826590

Following gene: 227826597

Centisome position: 6.08

GC content: 35.99

Gene sequence:

>1734_bases
ATGAGTAAAGTTTTCAAATCTCTCTTGCCAAAAGGCAAACCTAATGTGAAGCAATTACCTATCACAGAACTACCTATAAC
ACTCTATCCAGTTAGCCCTTTTCCAGAAGAGGTAACTACAATTGCGGCAGACTATGAAGTGAATATACTAAGCTTAGCCC
CAGAGGATGTCAAGTCTAACATAACCCGAAATAATATAGAGTTAGTATTGCCGAATCCTCACATCTTTGTTACCTTTGAT
GAAAGGAAGGGTATATATAAATATGTTTTATTAGAACCACCAGTTAATGATACCATTTATAACATGTACAATATATTCAT
AGAAGAGATAGAGAGAGAATTACTTTCTAAAACACCCTCACTTGACCTTGCAAAAATTATATTTGAACTTAATAAGAAAA
GATCTGATCTTAATATTATTCAAGAGAAAAGAGGAGATATATATATATTAACTACAAACGCAAGAGTTACGTTATATTAC
TTGTTAAGGAACATGTTCGGATATAATGTATTAACTTCACTGGTTGCTGATAAGAATATAGAAGATATTTCTGTTTCTGG
TTTGAATAATCCAGTCTATGTATATCATAGAAGTTATGAATACATACCAACTAATATTATGTTTACTAAGAATATGAAGA
TATCTCCGCAGCTTAATATTATGATAGATGGCGAGGAATTGTTGGATCAATTAGTGCTAAGAATGCTTTCAACCACAGGT
AAGTCAATTTCAGTTGCTGAACCAATACAAGACGGTATGCTACCAAATGGTGATAGGATTGCCGCAACATTTAGGCGTGA
GGTATCAGCCAGTGGTTCTTCAGTAGTGATAAGGAGATTCAGTGAAAGGCCTATTACGATACTAGATTTAATCAATTCTG
GCACCTTATCTCCAGAGTTGGCCGCATACCTATGGTATGGAATGGATCTGAGAATGAGTGTTATGTCAATAGGGGTTACT
GGTGCTGGAAAGACCACTTTGCTTAATGCAGTTCTGAATCTAGTTAAAGAAAGTATGAAGATTGTCTCCATAGAAGATAT
CCCGGAAATTAGATTAGCCCATACTAATTGGGTCCAACTGTACGCTAGGCCAGCATATGCAGGAGTAGGCAAAGAGATTT
CATTAATGGATTTGTTAAAATTATCCCTCAGATATAGGCCAGATATAATAGTAGTAGGCGAGATAAGAGGGCAAGAGGCG
TACGTATTATTCCAAGCGATATCAACTGGTCATGGAGGTGCTACGACGTTCCACGCATATAATACCGACTCTGCAATAAA
GAGGCTTATGAATGAGCCTCTTAACATTCCACAAGAGTGGATACCTATGATGAACATAATGATGACGATTAGAAGGTTAC
CAGTATATGTAGGCGAGAAGATCGTTCTAAGGAGACGTGTTGTAGCAGTTGATGAAATAGTTAGTTGGAATGATTATAGA
AGGGTCTCGAGTTGGGACCCAAAAGGTGACGTGTTTACAATCAATCTTGATTCTGCCAGAGTATTAAGAAATAGAATTGA
GGAAGCTGGTCTTAATATAGATGATGTTAAGAGGGAGATGGAGAGGAGAGCATTATTCCTAAAATTATTGGCATCTTCTA
GAGAGATAATACAAAGTGAGGAGAGTTATAAGCTTGTGAAGAAATATATAATAAAATACAGTTTAAAGCCAGAAGAAGCT
CTAAAAGAAGTTCAAACAATGGCTGGAGTAAAAACTATAGAACTAAAAGAATAA

Upstream 100 bases:

>100_bases
TAACTGCAATCTTAGTTGTCATATCATTACATATATCCCTAATACCTTCTATATCTCCATCATCAACATCTTTATAATCT
AGATGGAAGAGTGATATATA

Downstream 100 bases:

>100_bases
TTGAAAATAAAATTAATAATAAAGACGAAAGTGTGTTATTGAAAAGACTATTAACATATGCCATAATCGCTAGAATAACT
CCTAATATTCTTATACTCAA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSKVFKSLLPKGKPNVKQLPITELPITLYPVSPFPEEVTTIAADYEVNILSLAPEDVKSNITRNNIELVLPNPHIFVTFD
ERKGIYKYVLLEPPVNDTIYNMYNIFIEEIERELLSKTPSLDLAKIIFELNKKRSDLNIIQEKRGDIYILTTNARVTLYY
LLRNMFGYNVLTSLVADKNIEDISVSGLNNPVYVYHRSYEYIPTNIMFTKNMKISPQLNIMIDGEELLDQLVLRMLSTTG
KSISVAEPIQDGMLPNGDRIAATFRREVSASGSSVVIRRFSERPITILDLINSGTLSPELAAYLWYGMDLRMSVMSIGVT
GAGKTTLLNAVLNLVKESMKIVSIEDIPEIRLAHTNWVQLYARPAYAGVGKEISLMDLLKLSLRYRPDIIVVGEIRGQEA
YVLFQAISTGHGGATTFHAYNTDSAIKRLMNEPLNIPQEWIPMMNIMMTIRRLPVYVGEKIVLRRRVVAVDEIVSWNDYR
RVSSWDPKGDVFTINLDSARVLRNRIEEAGLNIDDVKREMERRALFLKLLASSREIIQSEESYKLVKKYIIKYSLKPEEA
LKEVQTMAGVKTIELKE

Sequences:

>Translated_577_residues
MSKVFKSLLPKGKPNVKQLPITELPITLYPVSPFPEEVTTIAADYEVNILSLAPEDVKSNITRNNIELVLPNPHIFVTFD
ERKGIYKYVLLEPPVNDTIYNMYNIFIEEIERELLSKTPSLDLAKIIFELNKKRSDLNIIQEKRGDIYILTTNARVTLYY
LLRNMFGYNVLTSLVADKNIEDISVSGLNNPVYVYHRSYEYIPTNIMFTKNMKISPQLNIMIDGEELLDQLVLRMLSTTG
KSISVAEPIQDGMLPNGDRIAATFRREVSASGSSVVIRRFSERPITILDLINSGTLSPELAAYLWYGMDLRMSVMSIGVT
GAGKTTLLNAVLNLVKESMKIVSIEDIPEIRLAHTNWVQLYARPAYAGVGKEISLMDLLKLSLRYRPDIIVVGEIRGQEA
YVLFQAISTGHGGATTFHAYNTDSAIKRLMNEPLNIPQEWIPMMNIMMTIRRLPVYVGEKIVLRRRVVAVDEIVSWNDYR
RVSSWDPKGDVFTINLDSARVLRNRIEEAGLNIDDVKREMERRALFLKLLASSREIIQSEESYKLVKKYIIKYSLKPEEA
LKEVQTMAGVKTIELKE
>Mature_576_residues
SKVFKSLLPKGKPNVKQLPITELPITLYPVSPFPEEVTTIAADYEVNILSLAPEDVKSNITRNNIELVLPNPHIFVTFDE
RKGIYKYVLLEPPVNDTIYNMYNIFIEEIERELLSKTPSLDLAKIIFELNKKRSDLNIIQEKRGDIYILTTNARVTLYYL
LRNMFGYNVLTSLVADKNIEDISVSGLNNPVYVYHRSYEYIPTNIMFTKNMKISPQLNIMIDGEELLDQLVLRMLSTTGK
SISVAEPIQDGMLPNGDRIAATFRREVSASGSSVVIRRFSERPITILDLINSGTLSPELAAYLWYGMDLRMSVMSIGVTG
AGKTTLLNAVLNLVKESMKIVSIEDIPEIRLAHTNWVQLYARPAYAGVGKEISLMDLLKLSLRYRPDIIVVGEIRGQEAY
VLFQAISTGHGGATTFHAYNTDSAIKRLMNEPLNIPQEWIPMMNIMMTIRRLPVYVGEKIVLRRRVVAVDEIVSWNDYRR
VSSWDPKGDVFTINLDSARVLRNRIEEAGLNIDDVKREMERRALFLKLLASSREIIQSEESYKLVKKYIIKYSLKPEEAL
KEVQTMAGVKTIELKE

Specific function: Unknown

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=174, Percent_Identity=31.6091954022989, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65723; Mature: 65591

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVFKSLLPKGKPNVKQLPITELPITLYPVSPFPEEVTTIAADYEVNILSLAPEDVKSN
CHHHHHHHCCCCCCCCCCCCCEECCEEEEECCCCCHHHHEEEECCEEEEEEECCHHHHCC
ITRNNIELVLPNPHIFVTFDERKGIYKYVLLEPPVNDTIYNMYNIFIEEIERELLSKTPS
CCCCCCEEEECCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LDLAKIIFELNKKRSDLNIIQEKRGDIYILTTNARVTLYYLLRNMFGYNVLTSLVADKNI
CHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
EDISVSGLNNPVYVYHRSYEYIPTNIMFTKNMKISPQLNIMIDGEELLDQLVLRMLSTTG
CEEEEECCCCCEEEEECCCEECCCEEEEECCEEECCEEEEEECHHHHHHHHHHHHHHCCC
KSISVAEPIQDGMLPNGDRIAATFRREVSASGSSVVIRRFSERPITILDLINSGTLSPEL
CCEEECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCHHH
AAYLWYGMDLRMSVMSIGVTGAGKTTLLNAVLNLVKESMKIVSIEDIPEIRLAHTNWVQL
HHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEE
YARPAYAGVGKEISLMDLLKLSLRYRPDIIVVGEIRGQEAYVLFQAISTGHGGATTFHAY
EECCHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEEE
NTDSAIKRLMNEPLNIPQEWIPMMNIMMTIRRLPVYVGEKIVLRRRVVAVDEIVSWNDYR
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCC
RVSSWDPKGDVFTINLDSARVLRNRIEEAGLNIDDVKREMERRALFLKLLASSREIIQSE
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
ESYKLVKKYIIKYSLKPEEALKEVQTMAGVKTIELKE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECC
>Mature Secondary Structure 
SKVFKSLLPKGKPNVKQLPITELPITLYPVSPFPEEVTTIAADYEVNILSLAPEDVKSN
HHHHHHHCCCCCCCCCCCCCEECCEEEEECCCCCHHHHEEEECCEEEEEEECCHHHHCC
ITRNNIELVLPNPHIFVTFDERKGIYKYVLLEPPVNDTIYNMYNIFIEEIERELLSKTPS
CCCCCCEEEECCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LDLAKIIFELNKKRSDLNIIQEKRGDIYILTTNARVTLYYLLRNMFGYNVLTSLVADKNI
CHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
EDISVSGLNNPVYVYHRSYEYIPTNIMFTKNMKISPQLNIMIDGEELLDQLVLRMLSTTG
CEEEEECCCCCEEEEECCCEECCCEEEEECCEEECCEEEEEECHHHHHHHHHHHHHHCCC
KSISVAEPIQDGMLPNGDRIAATFRREVSASGSSVVIRRFSERPITILDLINSGTLSPEL
CCEEECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCHHH
AAYLWYGMDLRMSVMSIGVTGAGKTTLLNAVLNLVKESMKIVSIEDIPEIRLAHTNWVQL
HHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEE
YARPAYAGVGKEISLMDLLKLSLRYRPDIIVVGEIRGQEAYVLFQAISTGHGGATTFHAY
EECCHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEEE
NTDSAIKRLMNEPLNIPQEWIPMMNIMMTIRRLPVYVGEKIVLRRRVVAVDEIVSWNDYR
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCC
RVSSWDPKGDVFTINLDSARVLRNRIEEAGLNIDDVKREMERRALFLKLLASSREIIQSE
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
ESYKLVKKYIIKYSLKPEEALKEVQTMAGVKTIELKE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]