Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 227826552

GI number: 227826552

Start: 125161

End: 126006

Strand: Direct

Name: ispB [H]

Synonym: M1425_0142

Alternate gene names: 227826552

Gene position: 125161-126006 (Clockwise)

Preceding gene: 227826551

Following gene: 227826553

Centisome position: 4.8

GC content: 34.28

Gene sequence:

>846_bases
TTGAGCATCATAGAGTTTTGGCTAGAAGCTAAGACTACTATAGACAAATTAATCGAACAATTTCTAAATTCAAATAGGGA
TTGGGATCTAGTTGATATAAGTACTTATATTCTTAAAGATGGTAAGCGCTTTAGGGGAACTTTAAACATGTTCTTTACTG
TTGCTTTAGGTGGAGATATTAAGGACTCTTACGGTGGTGCTTTGGCTATTGAAATTTTACATTCTGCTTCTTTAGCTTTA
GACGATATAGTTGACCTTGACGTGACTAGGAGAGGTGATAAAGCTGCGTGGGTTATATACGGAAATAGGAAAGTAATATT
CATAACCAATTATCTTATTCCCACTGCTCTCAGGATAATTCAGACTTCTTATGGTGACGATGCGTTAAATACGAGCATTG
AGTTGTGGAAAGACACTTCAGTTGGCGCTTTAAGGGATATGTATGATAATAATGATTACATACGAACAATTGAGTTAAAA
ACTGGTAGTTTATTTAAGCTTTCCACAGTGTTATCTGCATATGCTTCTAAGCACTATAATACGAAACAACAGATGTTGGA
TGTTGGCAAGTATTTAGGAATAATCTATCAAGTAATAGACGACTTTGTTGACTATAAAACTAAGAAATTAGAGGAGATAG
ATGGTAGTGCCAAGCAATTATTCAAGTATTACAGAGAGGGAAAGTTGGAAGAGTATGTTAGGTCAGTATATTTAGAATAT
AAGCAAAAATATGATGAGCTAATAAGTAACATTCCCTTCCAATCTAAATATATTAGTGAGATACGATCCCTTCCAGAGTT
CTTAGCTAACGGTCTTCTAAAGGAGGCTAACATAGATAAGATTTAA

Upstream 100 bases:

>100_bases
GGTTACTGATGTTCTGATAGATGTAGACCCACTATTAGCACCCATATATTTAAGTATGATTATGTTTTACTTAAAAAAGA
AATAAAGGAGGTTCTGCTGA

Downstream 100 bases:

>100_bases
GAAATTTAAATTCAAGGTCTTTTTGGTGAATTATTATTAGACTTGCAGTAATCCATGAATCTGAGAAAGTAACTAAGGCT
TCTAAGCAACTTTTATTGGA

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSIIEFWLEAKTTIDKLIEQFLNSNRDWDLVDISTYILKDGKRFRGTLNMFFTVALGGDIKDSYGGALAIEILHSASLAL
DDIVDLDVTRRGDKAAWVIYGNRKVIFITNYLIPTALRIIQTSYGDDALNTSIELWKDTSVGALRDMYDNNDYIRTIELK
TGSLFKLSTVLSAYASKHYNTKQQMLDVGKYLGIIYQVIDDFVDYKTKKLEEIDGSAKQLFKYYREGKLEEYVRSVYLEY
KQKYDELISNIPFQSKYISEIRSLPEFLANGLLKEANIDKI

Sequences:

>Translated_281_residues
MSIIEFWLEAKTTIDKLIEQFLNSNRDWDLVDISTYILKDGKRFRGTLNMFFTVALGGDIKDSYGGALAIEILHSASLAL
DDIVDLDVTRRGDKAAWVIYGNRKVIFITNYLIPTALRIIQTSYGDDALNTSIELWKDTSVGALRDMYDNNDYIRTIELK
TGSLFKLSTVLSAYASKHYNTKQQMLDVGKYLGIIYQVIDDFVDYKTKKLEEIDGSAKQLFKYYREGKLEEYVRSVYLEY
KQKYDELISNIPFQSKYISEIRSLPEFLANGLLKEANIDKI
>Mature_280_residues
SIIEFWLEAKTTIDKLIEQFLNSNRDWDLVDISTYILKDGKRFRGTLNMFFTVALGGDIKDSYGGALAIEILHSASLALD
DIVDLDVTRRGDKAAWVIYGNRKVIFITNYLIPTALRIIQTSYGDDALNTSIELWKDTSVGALRDMYDNNDYIRTIELKT
GSLFKLSTVLSAYASKHYNTKQQMLDVGKYLGIIYQVIDDFVDYKTKKLEEIDGSAKQLFKYYREGKLEEYVRSVYLEYK
QKYDELISNIPFQSKYISEIRSLPEFLANGLLKEANIDKI

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789578, Length=202, Percent_Identity=27.7227722772277, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325188, Length=210, Percent_Identity=26.1904761904762, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 32374; Mature: 32243

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIEFWLEAKTTIDKLIEQFLNSNRDWDLVDISTYILKDGKRFRGTLNMFFTVALGGDI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCHHHHHHHEEEEEEEECCCC
KDSYGGALAIEILHSASLALDDIVDLDVTRRGDKAAWVIYGNRKVIFITNYLIPTALRII
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEEECHHHHHHHHHH
QTSYGDDALNTSIELWKDTSVGALRDMYDNNDYIRTIELKTGSLFKLSTVLSAYASKHYN
HHCCCCHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
TKQQMLDVGKYLGIIYQVIDDFVDYKTKKLEEIDGSAKQLFKYYREGKLEEYVRSVYLEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
KQKYDELISNIPFQSKYISEIRSLPEFLANGLLKEANIDKI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHCCCCCCCC
>Mature Secondary Structure 
SIIEFWLEAKTTIDKLIEQFLNSNRDWDLVDISTYILKDGKRFRGTLNMFFTVALGGDI
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCHHHHHHHEEEEEEEECCCC
KDSYGGALAIEILHSASLALDDIVDLDVTRRGDKAAWVIYGNRKVIFITNYLIPTALRII
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEEECHHHHHHHHHH
QTSYGDDALNTSIELWKDTSVGALRDMYDNNDYIRTIELKTGSLFKLSTVLSAYASKHYN
HHCCCCHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
TKQQMLDVGKYLGIIYQVIDDFVDYKTKKLEEIDGSAKQLFKYYREGKLEEYVRSVYLEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
KQKYDELISNIPFQSKYISEIRSLPEFLANGLLKEANIDKI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]