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The map label for this gene is lpdA [C]

Identifier: 227826505

GI number: 227826505

Start: 80797

End: 82122

Strand: Direct

Name: lpdA [C]

Synonym: M1425_0094

Alternate gene names: 227826505

Gene position: 80797-82122 (Clockwise)

Preceding gene: 227826501

Following gene: 227826506

Centisome position: 3.1

GC content: 37.86

Gene sequence:

>1326_bases
ATGGAAAGGCTTATTATTATAGGTGGTGGAGCTGCTGGAATGACAGCTGCCTCATGGGCTAGAAGGCTTAAGCCAAATAT
TGATATAACAGTCTTTGAATCCACTAAGATGGTCAGTCATGCACCTTGTGGTATTCCCTATTTTACTGAGGGTCTTTTTG
ACGATGAAAACTTATTCATGACATATACTCCAGAATATTTTGCTGAAAAGAGAAAGATAAATGTTAAGATAAATTCTAAG
GTGGAGGAGGTAGATTTAAGATCTAGAACTATCACAGTAAGGGAAAATCAGGAAAATAAAAAATACGAATTTGATTATCT
ATTACTTTCGACAGGTGCTAAACCCAAAAAGCTAAATGCAGAGGGAGACAGAATTTTCTATGTTCATCATCCAGCAGACG
CCTCATACATAAGGCAAAAATTATGGAGTTTTGATAGGATTGCAATAATTGGCGGGGGTATATTAGGCATTGAGATGGCA
GAAGCACTAAGAGCTAGAGGAAAGAAACTTGTTTTGATTCATAGGGGAAAATATTTGCTTAATAAAATGCTCGATGAAGA
TATGGGTAAGATAATAACAGATAAAGTTGGAAGCGAAATAGAGCTAAAGTTAAATGAGAGTTTAATAAGCGTTACAGAAA
GGGGAAGACTCATAGTAACAGATAAAGGAAAATATGAAGTAGATGCTACCGTAGTTGGTATAGGAGTTGAACCAAATGTC
GATCTAGTTAAAGATCAGCTAAAAATAGGGGAGACTGGCGCTATATGGGCTGATAACCATATGAGAACGAGCTTCGAAAA
CGTTTATGCAGCTGGAGATTCGACAGAATCGATAAATATTATTACCAAAAAACCCGATTGGGTTCCCTTTGCGCCAGTAG
CCAATAAAATGGGTTTCGTAGCTGGGAATAACATAGGCGGTAAAGATGTAACTTTCCCTGGAGTAATAGGGACGATGATA
ACTAAATTTGAGGAATACGTTATAGCCAAGACTGGTATTACTGAGAACGAGGCTAAACGTCACAACATCAAAACAGTTTC
AGCTACAGTCCATCATAAGACTAGGGCTAGATACTATCCTGGATCTAAAGATATCATAGTGAAGTTAATCGCCGAGGCTA
ATACTATGAGAATAATAGGTGCACAGATTATAGGAGAAGAGGAGGTTCTAGGAAGGTTAAATATGATGGCAGCTGTTATT
CAAAAAGGTTTCACTGCAGAAGAGTTATTCTTTGTAGAAACGGGATATGTTCCACCCGTAAATAGAGTATGGGATGCGAT
TACTTTAGCAGCGAGAAAACTATATACTGGCATTAGCGGGGAATAG

Upstream 100 bases:

>100_bases
TTTATTCAGCTCAGCCATAGTATTATCTAATCTTTCAACAGCTTTCATGTGATATATTTAGACCGTAAATATAATAAGGT
TTCTGTGAATTTAATCAGTT

Downstream 100 bases:

>100_bases
ACCTAAGCTTTCTGGGTCTTCTTTCAGTATTACCTCATATTTAAATATTACCATAATAAAATATGATATGAAAGATGGCT
AAAAAGTTAGAGCTTCCGGT

Product: CoA-disulfide reductase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MERLIIIGGGAAGMTAASWARRLKPNIDITVFESTKMVSHAPCGIPYFTEGLFDDENLFMTYTPEYFAEKRKINVKINSK
VEEVDLRSRTITVRENQENKKYEFDYLLLSTGAKPKKLNAEGDRIFYVHHPADASYIRQKLWSFDRIAIIGGGILGIEMA
EALRARGKKLVLIHRGKYLLNKMLDEDMGKIITDKVGSEIELKLNESLISVTERGRLIVTDKGKYEVDATVVGIGVEPNV
DLVKDQLKIGETGAIWADNHMRTSFENVYAAGDSTESINIITKKPDWVPFAPVANKMGFVAGNNIGGKDVTFPGVIGTMI
TKFEEYVIAKTGITENEAKRHNIKTVSATVHHKTRARYYPGSKDIIVKLIAEANTMRIIGAQIIGEEEVLGRLNMMAAVI
QKGFTAEELFFVETGYVPPVNRVWDAITLAARKLYTGISGE

Sequences:

>Translated_441_residues
MERLIIIGGGAAGMTAASWARRLKPNIDITVFESTKMVSHAPCGIPYFTEGLFDDENLFMTYTPEYFAEKRKINVKINSK
VEEVDLRSRTITVRENQENKKYEFDYLLLSTGAKPKKLNAEGDRIFYVHHPADASYIRQKLWSFDRIAIIGGGILGIEMA
EALRARGKKLVLIHRGKYLLNKMLDEDMGKIITDKVGSEIELKLNESLISVTERGRLIVTDKGKYEVDATVVGIGVEPNV
DLVKDQLKIGETGAIWADNHMRTSFENVYAAGDSTESINIITKKPDWVPFAPVANKMGFVAGNNIGGKDVTFPGVIGTMI
TKFEEYVIAKTGITENEAKRHNIKTVSATVHHKTRARYYPGSKDIIVKLIAEANTMRIIGAQIIGEEEVLGRLNMMAAVI
QKGFTAEELFFVETGYVPPVNRVWDAITLAARKLYTGISGE
>Mature_441_residues
MERLIIIGGGAAGMTAASWARRLKPNIDITVFESTKMVSHAPCGIPYFTEGLFDDENLFMTYTPEYFAEKRKINVKINSK
VEEVDLRSRTITVRENQENKKYEFDYLLLSTGAKPKKLNAEGDRIFYVHHPADASYIRQKLWSFDRIAIIGGGILGIEMA
EALRARGKKLVLIHRGKYLLNKMLDEDMGKIITDKVGSEIELKLNESLISVTERGRLIVTDKGKYEVDATVVGIGVEPNV
DLVKDQLKIGETGAIWADNHMRTSFENVYAAGDSTESINIITKKPDWVPFAPVANKMGFVAGNNIGGKDVTFPGVIGTMI
TKFEEYVIAKTGITENEAKRHNIKTVSATVHHKTRARYYPGSKDIIVKLIAEANTMRIIGAQIIGEEEVLGRLNMMAAVI
QKGFTAEELFFVETGYVPPVNRVWDAITLAARKLYTGISGE

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=336, Percent_Identity=22.9166666666667, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI226437568, Length=312, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI65787454, Length=312, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786307, Length=339, Percent_Identity=27.7286135693215, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789065, Length=352, Percent_Identity=22.7272727272727, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788892, Length=277, Percent_Identity=21.6606498194946, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 49098; Mature: 49098

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLIIIGGGAAGMTAASWARRLKPNIDITVFESTKMVSHAPCGIPYFTEGLFDDENLFM
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEECCCCCCCCHHHCCCCCCCCEEE
TYTPEYFAEKRKINVKINSKVEEVDLRSRTITVRENQENKKYEFDYLLLSTGAKPKKLNA
EECHHHHCCCEEEEEEECCCEEEEECCCEEEEEEECCCCCCEEEEEEEEECCCCCCEECC
EGDRIFYVHHPADASYIRQKLWSFDRIAIIGGGILGIEMAEALRARGKKLVLIHRGKYLL
CCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHH
NKMLDEDMGKIITDKVGSEIELKLNESLISVTERGRLIVTDKGKYEVDATVVGIGVEPNV
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCH
DLVKDQLKIGETGAIWADNHMRTSFENVYAAGDSTESINIITKKPDWVPFAPVANKMGFV
HHHHHHHHCCCCCCEEECCCCCCCHHHEEECCCCCCEEEEEECCCCCCCCCHHHCCCCEE
AGNNIGGKDVTFPGVIGTMITKFEEYVIAKTGITENEAKRHNIKTVSATVHHKTRARYYP
ECCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCHHHCCEEEEEEEEEHHHCCEECC
GSKDIIVKLIAEANTMRIIGAQIIGEEEVLGRLNMMAAVIQKGFTAEELFFVETGYVPPV
CCHHEEEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCH
NRVWDAITLAARKLYTGISGE
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MERLIIIGGGAAGMTAASWARRLKPNIDITVFESTKMVSHAPCGIPYFTEGLFDDENLFM
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEECCCCCCCCHHHCCCCCCCCEEE
TYTPEYFAEKRKINVKINSKVEEVDLRSRTITVRENQENKKYEFDYLLLSTGAKPKKLNA
EECHHHHCCCEEEEEEECCCEEEEECCCEEEEEEECCCCCCEEEEEEEEECCCCCCEECC
EGDRIFYVHHPADASYIRQKLWSFDRIAIIGGGILGIEMAEALRARGKKLVLIHRGKYLL
CCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHH
NKMLDEDMGKIITDKVGSEIELKLNESLISVTERGRLIVTDKGKYEVDATVVGIGVEPNV
HHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCH
DLVKDQLKIGETGAIWADNHMRTSFENVYAAGDSTESINIITKKPDWVPFAPVANKMGFV
HHHHHHHHCCCCCCEEECCCCCCCHHHEEECCCCCCEEEEEECCCCCCCCCHHHCCCCEE
AGNNIGGKDVTFPGVIGTMITKFEEYVIAKTGITENEAKRHNIKTVSATVHHKTRARYYP
ECCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCHHHCCEEEEEEEEEHHHCCEECC
GSKDIIVKLIAEANTMRIIGAQIIGEEEVLGRLNMMAAVIQKGFTAEELFFVETGYVPPV
CCHHEEEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCH
NRVWDAITLAARKLYTGISGE
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA