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The map label for this gene is ybeK [C]

Identifier: 227826468

GI number: 227826468

Start: 48281

End: 49204

Strand: Direct

Name: ybeK [C]

Synonym: M1425_0057

Alternate gene names: 227826468

Gene position: 48281-49204 (Clockwise)

Preceding gene: 227826466

Following gene: 227826470

Centisome position: 1.85

GC content: 35.93

Gene sequence:

>924_bases
ATGAGGAAGGTCATATTAGATTCAGATACAGCTAGCGATGATACTATAGCCATATTGCTTGCATCTAGATATTTCCAATT
ATTAGGTGTGACAATAGTTGCTGGAAATGTAAATTATAATCAAGAGGTTAAGAATGCACTTTTCACTTTAGAGTACATTG
GTAAGCAAGACGTACCAGTTTATTTAGGATCACAAAGACCTATTTTGGGAAATTGGAGAACAGTTGAGGAAGTCCATGGA
AGTAACGGAATGAGTGATTGGGACTATCCCGAACCTACTAAAAGGCCAGAGAAAGAGCATGCAATAGATGCTATACTCAG
ATTGTCGAAAGAATACGACGGGGAATTGGAAATACTTGCAGTTTCCCCTTTAACAAATATTGCTTTGGCTTATCTTAAAG
ATCCGTCAGTTGTCAAGCGAATAAGAAAGATTTGGATAATGGGTGGTGCTTTTTCAAAGGGTAATACTACCCCTATAGCG
GAGTTCAATTTCTGGGTAGATCCCGAGGCTGCTAAAATAGTTTTAGATGCAGGATTTGATATTACCGTCGTTCCTTGGGA
AGTCGCAGAAACAAGTGGTTCTTTAAACGAGAGAGATTGGGAATTTATCTCCAAACTCAATACTAAGCTCTCAAAATTCT
TTATTAATGTCAATAAGACATTAAAGGACTATACTACTAAGAATCAAGGTATATCTGGTAGTACTCATCCAGATTCCTTA
ACAGTTTCTATAGCCTATGATAGATCTATTATCTCAGAGTCTTCTCTAAAGTATATTGATTTAGAATTATGTTCAAAGTC
AAGAGGAGCAATGTTAATAGACTGGTACAGTTTACATAAAAATAAGCCTAATGCTGAAATTGTATTAAAGGCAGATAGTA
ACAAGTTCAAAAATCTTCTATTCAGAACCCTTTCCGAGTTTTGA

Upstream 100 bases:

>100_bases
TTGTATAAATTCAATCAATTTCAAATTTTTATAATTTATTAAAACAGAGAAGACTTTAGATACTCGCAGTTTATTATATT
TGTCAAGGTGATAGAGTTTA

Downstream 100 bases:

>100_bases
ACAATCAGCGTATACTATAATGTTTACATTTTTTACGTCTAAGCCATTTTTAACTAGCTTATCCATGAAGTTATCCATTT
CAACATCTAAGTCAATAATT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRKVILDSDTASDDTIAILLASRYFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPVYLGSQRPILGNWRTVEEVHG
SNGMSDWDYPEPTKRPEKEHAIDAILRLSKEYDGELEILAVSPLTNIALAYLKDPSVVKRIRKIWIMGGAFSKGNTTPIA
EFNFWVDPEAAKIVLDAGFDITVVPWEVAETSGSLNERDWEFISKLNTKLSKFFINVNKTLKDYTTKNQGISGSTHPDSL
TVSIAYDRSIISESSLKYIDLELCSKSRGAMLIDWYSLHKNKPNAEIVLKADSNKFKNLLFRTLSEF

Sequences:

>Translated_307_residues
MRKVILDSDTASDDTIAILLASRYFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPVYLGSQRPILGNWRTVEEVHG
SNGMSDWDYPEPTKRPEKEHAIDAILRLSKEYDGELEILAVSPLTNIALAYLKDPSVVKRIRKIWIMGGAFSKGNTTPIA
EFNFWVDPEAAKIVLDAGFDITVVPWEVAETSGSLNERDWEFISKLNTKLSKFFINVNKTLKDYTTKNQGISGSTHPDSL
TVSIAYDRSIISESSLKYIDLELCSKSRGAMLIDWYSLHKNKPNAEIVLKADSNKFKNLLFRTLSEF
>Mature_307_residues
MRKVILDSDTASDDTIAILLASRYFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPVYLGSQRPILGNWRTVEEVHG
SNGMSDWDYPEPTKRPEKEHAIDAILRLSKEYDGELEILAVSPLTNIALAYLKDPSVVKRIRKIWIMGGAFSKGNTTPIA
EFNFWVDPEAAKIVLDAGFDITVVPWEVAETSGSLNERDWEFISKLNTKLSKFFINVNKTLKDYTTKNQGISGSTHPDSL
TVSIAYDRSIISESSLKYIDLELCSKSRGAMLIDWYSLHKNKPNAEIVLKADSNKFKNLLFRTLSEF

Specific function: Unknown

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=306, Percent_Identity=30.718954248366, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=29.6052631578947, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786213, Length=159, Percent_Identity=35.8490566037736, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565474, Length=298, Percent_Identity=32.8859060402685, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17565698, Length=290, Percent_Identity=30, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=241, Percent_Identity=31.1203319502075, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583915, Length=327, Percent_Identity=27.5229357798165, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24641839, Length=334, Percent_Identity=30.2395209580838, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641837, Length=240, Percent_Identity=32.5, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34579; Mature: 34579

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVILDSDTASDDTIAILLASRYFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPV
CCEEEECCCCCCCCEEEEHHHHHHHHHHEEEEEECCCCCCHHHHHHHEEHHHCCCCCCCE
YLGSQRPILGNWRTVEEVHGSNGMSDWDYPEPTKRPEKEHAIDAILRLSKEYDGELEILA
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
VSPLTNIALAYLKDPSVVKRIRKIWIMGGAFSKGNTTPIAEFNFWVDPEAAKIVLDAGFD
ECCHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCC
ITVVPWEVAETSGSLNERDWEFISKLNTKLSKFFINVNKTLKDYTTKNQGISGSTHPDSL
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHCCCCCCCCCCCCCCEE
TVSIAYDRSIISESSLKYIDLELCSKSRGAMLIDWYSLHKNKPNAEIVLKADSNKFKNLL
EEEEEECCHHHCCCCCEEEEEEECCCCCCEEEEEEEEHHCCCCCCEEEEEECCHHHHHHH
FRTLSEF
HHHHHCC
>Mature Secondary Structure
MRKVILDSDTASDDTIAILLASRYFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPV
CCEEEECCCCCCCCEEEEHHHHHHHHHHEEEEEECCCCCCHHHHHHHEEHHHCCCCCCCE
YLGSQRPILGNWRTVEEVHGSNGMSDWDYPEPTKRPEKEHAIDAILRLSKEYDGELEILA
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
VSPLTNIALAYLKDPSVVKRIRKIWIMGGAFSKGNTTPIAEFNFWVDPEAAKIVLDAGFD
ECCHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCC
ITVVPWEVAETSGSLNERDWEFISKLNTKLSKFFINVNKTLKDYTTKNQGISGSTHPDSL
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHCCCCCCCCCCCCCCEE
TVSIAYDRSIISESSLKYIDLELCSKSRGAMLIDWYSLHKNKPNAEIVLKADSNKFKNLL
EEEEEECCHHHCCCCCEEEEEEECCCCCCEEEEEEEEHHCCCCCCEEEEEECCHHHHHHH
FRTLSEF
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA