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The map label for this gene is dat2 [H]

Identifier: 227817992

GI number: 227817992

Start: 4962266

End: 4963138

Strand: Direct

Name: dat2 [H]

Synonym: BAMEG_5518

Alternate gene names: 227817992

Gene position: 4962266-4963138 (Clockwise)

Preceding gene: 227817991

Following gene: 227817993

Centisome position: 94.88

GC content: 37.46

Gene sequence:

>873_bases
TTGGCATATGAAAGATTTGTACTTTGGAATGATGCAATTATTGATACAACGAAGCAAAAAACGTACATAGAACTTGAAGA
AAGAGGCTTGCAGTTTGGAGATGGTGTCTACGAGGTTATTCGTCTATATAAAGGGAACTTCCACTTATTAGATCCCCATA
TCACAAGATTATATCGCTTCATGGAAGAAATAGAATTAACACTCCCTTTCTCAAAAGCAGAACTGATTACCCTACTTTAT
AAACTAATCGAAAATAATAATTTCCACGAAGATGGAACGATTTATTTGCAAGTATCTCGTGGTGTACAAGCTCGTACCCA
TACGTTCTCATATGACGTCCCTCCGACAATCTATGCTTATATTACAAAGAAAGAAAGACCAGCGCTATGGATTGAATATG
GTGTACGTGCTATATCAGAACCGGATACACGCTGGCTACGCTGTGATATCAAATCATTAAATTTATTACCAAATATATTA
GCTGCTACGAAAGCGGAACGAAAAGGTTGTAAAGAAGCCCTTTTCGTACGAAATGGTACTGTAACTGAGGGAAGTCACTC
TAACTTCTTTCTCATTAAAAACGGAACTCTTTACACACATCCAGCTAATCACCTTATTTTAAATGGCATTATTCGTCAAT
ATGTCCTTTCTTTAGCGAAAACCCTTCGTATTCCAGTACAAGAAGAGCTTTTCAGCATTCGTGATGTTTATCAAGCGGAT
GAATGTTTCTTTACAGGAACGACGATTGAAATTTTGCCGATGACTCACCTTGATGGAACCGCAATCCAAGATGGTCAAGT
TGGCCCTATCACTAAAATGCTGCAAAGATCATTTTCTCAAAGTTTGTTACAATCCAACATGTCATCCTCTTAA

Upstream 100 bases:

>100_bases
TGTAAAGAGTTACTCACATAAAAATTACTATAGTGATTTTTATTCCGAATGAATTACAGTTTCACTTTACTTATGCACTT
ACGAAAGGATGGGACGAAAA

Downstream 100 bases:

>100_bases
ACACGAATAATTTCTATCCTTTCTACATTCAATATATGTATTTAAATGGAAATCAAGGTATAGATTTATGCCTTGATTTT
TTCTTGTAATCTTTTTGTCA

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELITLLY
KLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNIL
AATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVYQAD
ECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS

Sequences:

>Translated_290_residues
MAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELITLLY
KLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNIL
AATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVYQAD
ECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
>Mature_289_residues
AYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELITLLYK
LIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNILA
ATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVYQADE
CFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=293, Percent_Identity=28.6689419795222, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787338, Length=255, Percent_Identity=26.2745098039216, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33380; Mature: 33249

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRF
CCCCEEEEECCCEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCCEEEECHHHHHHHHH
MEEIELTLPFSKAELITLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAY
HHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEE
ITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNILAATKAERKGCKEALFVRNGT
EECCCCCEEEEECCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEEECCC
VTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVYQAD
EECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
CEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRF
CCCEEEEECCCEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCCEEEECHHHHHHHHH
MEEIELTLPFSKAELITLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAY
HHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEE
ITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNILAATKAERKGCKEALFVRNGT
EECCCCCEEEEECCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEEECCC
VTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVYQAD
EECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
CEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644261; 2914916; 7626635; 9538014; 9749913 [H]