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The map label for this gene is celB3 [H]

Identifier: 227817973

GI number: 227817973

Start: 4938363

End: 4939664

Strand: Reverse

Name: celB3 [H]

Synonym: BAMEG_5498

Alternate gene names: 227817973

Gene position: 4939664-4938363 (Counterclockwise)

Preceding gene: 227817974

Following gene: 227817972

Centisome position: 94.45

GC content: 38.79

Gene sequence:

>1302_bases
ATGATACGGTTTTTAGAGAAATATGTAATGCCGGTAGCAGGGAAGGTTGCAGAGCAGAGGCACTTGCAAGCAATTCGAGA
TGGAATTATTTTAACGATGCCTTTTTTAATCATTGGATCATTTTTCCTTATTATTAGTGCATTACCAATACCAGGCTATA
ATGATTTTATGGCAGGTTTGTTTGGGGAGAATTGGCAGAGGGCTTTGGGGTATCCAGTTAGTGCGACTTTTAATATAATG
GCTTTAATAGCTGTTTTTGGAATCGCTTACAGGCTTGGGGAATATTATAAAGTGGATGCTTTAGCATCCGGAGCATTGTC
CCTTGTGACGTTTTTACTTGTGACGCCATTTCAAGTTGCATATATTATACCAAGTACAAAAGAGAGTGTACTTGTAGAAG
GTGCTATCCCAGCTGCATTAATGGGAAGCCAAGGGTTGTTTGTAGCAATGATTATTGCACTTATATCTACTGAACTTTAT
CGGTTTATTGTACAAAAAAAGATAATTATAAAGATGCCAGAAACAGTTCCACCAGCCGTGACGCGCTCATTTGCGGCACT
TGTTCCAGGATTTATTGTTGTAACGGTTATTTGGATTGTACGCTTAATTATAGAAAATACTTCTTTTGGCAGTATCCATA
ATATTGTAGGGCAAATTTTGCAGGAACCACTTAGTGTACTTGGTGCTAGTCTTTGGGGCGCAATAATAGCAGTTATTCTC
GTTCATGTCCTTTGGTCTTGTGGAATTCATGGTGCTACTATTGTTGGTGGTGTAATGAGTCCTGTTTGGTTGTCGTTAAT
GGATCAAAACCGAGTTGCTTTCCAAGCGGGGCAAGATGTACCAAATACGATTACCGCACAGTTTTTTGATTTATGGATTT
ATATGGGCGGTTCCGGCGCAACACTAGCTTTAGTTGTCGGAATGTTATTGTTTGCACGAAGTCAGCAATTAAAAAGTTTA
GGGAGATTGTCAATTGCACCTGGTATATTTAATATTAATGAGATGGTAACTTTTGGTATGCCGATTGTAATGAACCCAAT
TTTATTAATTCCATTTATATTAGTTCCGGTTGTGTTAACGATCGTTTCTTACTTTGCAATGGAATGGGGATTAGTTGCTC
GCCCGAGTGGAGCTGCTGTACCTTGGACGACACCTATTCTTTTTAGTGGATATTTAGGATCGGGCGGGAAAATTTCAGGC
GTTGTTTTACAACTTGTTAACTTTGCGCTTGCATTCTTCATTTATTTACCGTTCTTAAAAATATGGGATAAACAAAAAGT
AGCGGAAGAAAAGGGGGAGTAA

Upstream 100 bases:

>100_bases
TACCTGTTGATGTAATTCAATCCGTCCATTACGGACTTTGTAATGGGGAAGCTATCTTGCAAGCAGCGTTGTCAATGAAA
CCATGAGGAGAGTGGGATAG

Downstream 100 bases:

>100_bases
AGAAGGAAAATGGATACGATAGAGACGAAAGCTTTTCATTTGATTTTGCATGGGGGAAATGCGAGAAGTTGTTCAATGGA
AGCAATTGATTGCGCGAAGC

Product: PTS system, cellobiose-specific IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIRFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLVTPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSVLGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE

Sequences:

>Translated_433_residues
MIRFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLVTPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSVLGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE
>Mature_433_residues
MIRFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGLFGENWQRALGYPVSATFNIM
ALIAVFGIAYRLGEYYKVDALASGALSLVTFLLVTPFQVAYIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELY
RFIVQKKIIIKMPETVPPAVTRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSVLGASLWGAIIAVIL
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSL
GRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLTIVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISG
VVLQLVNFALAFFIYLPFLKIWDKQKVAEEKGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=468, Percent_Identity=33.5470085470085, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 47103; Mature: 47103

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGENWQRALGYPVSATFNIMALIAVFGIAYRLGEYYKVDALASGALSLVTFLLVTPFQVA
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHE
YIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELYRFIVQKKIIIKMPETVPPAV
EEECCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
TRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSVLGASLWGAIIAVIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHEECCCCH
TLALVVGMLLFARSQQLKSLGRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISGVVLQLVNFALAFFIYLPFLK
HHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWDKQKVAEEKGE
HHHHHHHHHCCCC
>Mature Secondary Structure
MIRFLEKYVMPVAGKVAEQRHLQAIRDGIILTMPFLIIGSFFLIISALPIPGYNDFMAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGENWQRALGYPVSATFNIMALIAVFGIAYRLGEYYKVDALASGALSLVTFLLVTPFQVA
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHE
YIIPSTKESVLVEGAIPAALMGSQGLFVAMIIALISTELYRFIVQKKIIIKMPETVPPAV
EEECCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
TRSFAALVPGFIVVTVIWIVRLIIENTSFGSIHNIVGQILQEPLSVLGASLWGAIIAVIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVLWSCGIHGATIVGGVMSPVWLSLMDQNRVAFQAGQDVPNTITAQFFDLWIYMGGSGA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHEECCCCH
TLALVVGMLLFARSQQLKSLGRLSIAPGIFNINEMVTFGMPIVMNPILLIPFILVPVVLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IVSYFAMEWGLVARPSGAAVPWTTPILFSGYLGSGGKISGVVLQLVNFALAFFIYLPFLK
HHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWDKQKVAEEKGE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]