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The map label for this gene is ytzG [H]

Identifier: 227817496

GI number: 227817496

Start: 4503550

End: 4504194

Strand: Reverse

Name: ytzG [H]

Synonym: BAMEG_4988

Alternate gene names: 227817496

Gene position: 4504194-4503550 (Counterclockwise)

Preceding gene: 227817497

Following gene: 227817494

Centisome position: 86.12

GC content: 34.73

Gene sequence:

>645_bases
GTGAGATTAGATAAATTATTAGCAAATATGGGATATGGTAGTAGAAAAGAAGTAAAGAAATTATTGAAAGACGGCGTTGT
GAAAATTGATGGAATATCGGTGAAAGATGCAAAGGTTCATGTAAATGTAGAAGAACAAGAAGTTATGATTCACGGTGAAG
TTGTGGAATATAAAGAGTTTGTTTACTTAATGATGCATAAACCACAAGGCGTTATTTCAGCGACAGAAGATGATAATCAT
GAAACGGTAATAGATTTATTAGAGTTAGAGGATGCAATTTTTAATCCGTTCCCAGTTGGAAGACTTGATATTGATACAGA
AGGTTTCTTATTAATAACAAATGACGGGAAGTTATCACATCAATTGTTATCTCCGAAAAAGCATGTGCCGAAAAAATATT
ATGCACACGTTGCAGGAGTAGTAACAGAAGAGGATGTAAAAGAGTTTGCTAAAGGTGTTATTTTAGATGATGGCTATGAA
ACAAAGCCAGGTGCACTTACAATATTAAAAAGCGATGATATTTCTGAAATCGAGCTTGTCATTACAGAAGGAAAGTTCCA
TCAAGTGAAGCGTATGTTTGAAGCGGTAGGGGAAAAAAGTAGTCTACTTAAAGAGAACAGAGATGGGACCGTTAGTGTTA
GATGA

Upstream 100 bases:

>100_bases
AGATCCAACGTAAACTTGGCAATAGATTCAAAATAAAGTTGAAATCAAAAGGGGCGTAATTACGTCCCCTTTTCTATGAT
AAATAAAGAAGGTGAAAAAC

Downstream 100 bases:

>100_bases
AGAGCTAGAACTTGGACAATACCGAGAGTTAACAGATGAAGAAGTAGAGATGTTAAAAACATATCAAGTAGACAATGAGA
AATAGTGCTATTTTTAGGCG

Product: RNA pseudouridine synthase family protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGEKSSLLKENRDGTVSVR

Sequences:

>Translated_214_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGEKSSLLKENRDGTVSVR
>Mature_214_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGEKSSLLKENRDGTVSVR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=215, Percent_Identity=35.3488372093023, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787524, Length=226, Percent_Identity=32.7433628318584, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790453, Length=218, Percent_Identity=28.4403669724771, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081838, Length=151, Percent_Identity=29.1390728476821, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 24033; Mature: 24033

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEECCEEEEEECHHEEEEECHHHHHHHE
VYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSH
EEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEIELV
HHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEEEEE
ITEGKFHQVKRMFEAVGEKSSLLKENRDGTVSVR
EECCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEECCEEEEEECHHEEEEECHHHHHHHE
VYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSH
EEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEIELV
HHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEEEEE
ITEGKFHQVKRMFEAVGEKSSLLKENRDGTVSVR
EECCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]