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The map label for this gene is citZ [H]

Identifier: 227817379

GI number: 227817379

Start: 4403048

End: 4404196

Strand: Reverse

Name: citZ [H]

Synonym: BAMEG_4870

Alternate gene names: 227817379

Gene position: 4404196-4403048 (Counterclockwise)

Preceding gene: 227817380

Following gene: 227817378

Centisome position: 84.21

GC content: 36.73

Gene sequence:

>1149_bases
TTGGGAGAATTTTCAGGAAAAGGAGAGAATGTCATGACTGTTATTCGAGGTTTAGAAGGGGTAGTAGCAACAACATCATC
TGTGAGCTCTATTATTGATGATACATTAACTTATGCTGGGTATAATATTGATGATTTAGCTGAGAATGCTACATTTGAAG
AAGTAGTGTACTTATTATGGCATCGCAAGCTTCCTAACGAGCAAGAACTAGCGAAATTTAATGAGATTGTATCGGAATAC
TATAAAGTTCCAGGTGAGATTTTAGCATATTTGAAACAAGTAGATTTAAAAATCGCACATCCGATGTCTGTTTTACGAAC
TGCAATTTCCATGCTATCATTATACGATGAGAGCGCTGAAATTATGGATGAAAAGTCCAATTATTTGAAAGCGGTTAAAT
TACAGGCTCAAGTGGGTACTTTAGTTGCTGCTTATGCAAGAATCCGTAAAGGTTTAGATGTTGTTGAGCCTAGAAATGAT
TTATCATTAGCTGCAAACTTCTTGTACATGTTAAATGATCGCGAGCCAAATGAAGTTGAAATTGAAGCTTTTGATAAGGC
GCTTGTACTTCACGCAGATCATGAGTTAAACGCTTCTACATTTACTGCACGCGTTTGTGTAGCTACACTTTCAGATGTAT
ATTCTGGTATTACAGCAGCAATCGGTGCGTTAAAAGGTCCTCTTCACGGCGGGGCAAATGAAAATGTAATGAAGATGCTA
ACTGAAATTGGCGAAGAAGAAAATGTAGAATCATATATTCATAATGCTCTTCAAAATAAAGTGAAAATCATGGGCTTTGG
CCACCGTGTATATGAGCAGGGTGATCCGCGTGCAAAACATTTACGCGAAATGTCTAAGAGATTATGCGTGCTTTTAGGAG
AAGAGAAATGGTATAATATGTCTATCAAAATTGAAGACATTGTAACAAAAGAAAAAGGTCTTCCACCAAATGTTGATTTC
TATTCAGCTTCTGTATACCATTGTTTAGGAATTGACCATGACTTATTTACACCTATTTTTGCAATTAGCCGTATGTCAGG
CTGGTTAGCTCATATTCTAGAACAATATGAAAATAACCGTTTAATCCGTCCGCGTGCTGATTATAATGGACCAACACATC
AAGTGTATGTTCCAATTGCACAACGATAA

Upstream 100 bases:

>100_bases
AACATCGCAACAAATTTGCATGATCGTGCATTTTGCTTGCCTAAAGAGTGAACGGGCGTTCATAATTCAAAAGGGGAGCA
AATAGATATAAGGAGCAAGG

Downstream 100 bases:

>100_bases
GCGAATAACACTATTTTGATAGTGAAAATACGCTTTCAAAAGTCTGATTTTTTCGGAAAGATGTAATGATTAGAATATTT
TAATTTGACTAACGGTTTGA

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGEFSGKGENVMTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEY
YKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRND
LSLAANFLYMLNDREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKML
TEIGEEENVESYIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDF
YSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR

Sequences:

>Translated_382_residues
MGEFSGKGENVMTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEY
YKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRND
LSLAANFLYMLNDREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKML
TEIGEEENVESYIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDF
YSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR
>Mature_381_residues
GEFSGKGENVMTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEYY
KVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDL
SLAANFLYMLNDREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLT
EIGEEENVESYIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDFY
SASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=374, Percent_Identity=25.668449197861, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=35.8695652173913, Blast_Score=237, Evalue=7e-64,
Organism=Escherichia coli, GI1786939, Length=379, Percent_Identity=33.7730870712401, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17555174, Length=373, Percent_Identity=27.0777479892761, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=383, Percent_Identity=23.2375979112272, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=386, Percent_Identity=22.7979274611399, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325257, Length=180, Percent_Identity=31.1111111111111, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640126, Length=377, Percent_Identity=27.0557029177719, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640124, Length=377, Percent_Identity=27.0557029177719, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356863, Length=382, Percent_Identity=27.2251308900524, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 42921; Mature: 42789

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEFSGKGENVMTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
HRKLPNEQELAKFNEIVSEYYKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAE
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLNDREPNEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCEEE
IEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKML
HEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TEIGEEENVESYIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNM
HHCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEE
SIKIEDIVTKEKGLPPNVDFYSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNR
EEEEHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIRPRADYNGPTHQVYVPIAQR
EECCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
GEFSGKGENVMTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
HRKLPNEQELAKFNEIVSEYYKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAE
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLNDREPNEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCEEE
IEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKML
HEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TEIGEEENVESYIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNM
HHCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEE
SIKIEDIVTKEKGLPPNVDFYSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNR
EEEEHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIRPRADYNGPTHQVYVPIAQR
EECCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]