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The map label for this gene is yitJ [H]

Identifier: 227817030

GI number: 227817030

Start: 4073488

End: 4075323

Strand: Reverse

Name: yitJ [H]

Synonym: BAMEG_4513

Alternate gene names: 227817030

Gene position: 4075323-4073488 (Counterclockwise)

Preceding gene: 227817035

Following gene: 227817029

Centisome position: 77.92

GC content: 38.45

Gene sequence:

>1836_bases
GTGAAATTACTAGATTTATTGTCAAAAGGAATTGTAATAGGTGATGGTGCGGTTGGAACATTATTACATTCACACGGTTT
GCAAAGTAGTTTTGAAGAATTGAATATATCTGATCCAGATTTAATTATATCGATTCATAAGCAATATGTAGCTGCTGGGG
CAGATGTAATTCAAACAAATACGTACGGAGCAAATGAAGCGAAATTGCGTATGTATGGTTTAGAAAATCAAGTTACTGAA
ATTAATAGAGCCGCAGTAAAACTGGCGAAAGCATCTGTTACAGACAGAAATGCAATTTTAGGAACAATAGGTGGTATGAA
ACATATTGGAGCGGTTACGACAACTGATATGGAGCGAGAATTTATGTTACTGGAACAAGCAAGTGCATTATTAGAAGAAC
AGGTTGATGGACTGCTATTAGAGACTTTTTATGATGAGTTTGAATTACTCCATGCTGTTCAGGTGTTACGAAAAGAAACG
AATATTCCAATTGTAGCGCAATTAGCGTTACATGAGGCAGGTACGACTCAAAATGGAAATGATGTAAATGAAATATTAAA
ACAGCTTTTAGATTACGGTGCAAATGTTGTTGGATTGAACTGCCAACTAGGGCCACTTCATATGACGGAAGCTTTTAAAA
TGATATCGATTCCGAAAAATGGTTACTTGTCAGCGTATCCAAATGCAGGTCTCCCAAATTATGTGGAGGGACGTTATGTA
TACGAGGGGAGTCCAGCTTATTTCGAAGCGATGACACCGAAATTTATTGAGCAAGGTATTCGGTTATTGGGTGGTTGTTG
TGGCACGACTCCAGAACATATTGAAAGTATGAAACGTGCGACTCTAAATGTTACACCTGTAATAGAAAAAGATACGATTC
AAAGACCGAAAGTAGTTCATACACACGAGAAACGTTCAAAAGCTCATGTCACTTTAGCAGAGAAAGCAAAAAAACAAACG
ACAGTAGTAGTAGTAGAATTAGATCCACCGAAAACGTTAGATACGCAGCGATTTTTTGAAGGCGCAAGAGCATTGAAAAG
GGCTGGAGCAGATGCTATTACTCTAGCAGATAATTCATTAGCATCGCCTCGTATCTCAAATATGGCAATGGGAGCATTAT
TAACGAAGCACGATATTCCGGTATTGACGCATTTAACGTGTAGAGACCATAACGTCATCGGACTACAATCTCACTTGCTA
GGCTTGTCAGCGTTAGGTATGGAAGAAGTATTAGCTTTAACTGGTGATCCAGCACGCGTTGGTGATTTTCCAGGTGCCAC
TTCTGTATATGATTTATCCTCTATTGAGCTCATTAAAATGATTAAAGAAATGAACGATGGGCGTTCTATTTTAGGGAAAT
CGATAGGTCCAGCAACAAGGTTTTCAGTAGGCGGCGCGTTTAATCCGCATGTAAGACATTTAAAAGCAGCGGTTAAGCGA
ATGGAACGGAAAATAGATGCTGGAGCGGAATATTTCTTAACGCAGCCGATTTATGATATCGCGTTAATCGAAGAAGTGTA
CGAAGCGACGAAGCATTTGGAACAACCTATTTTTATTGGCATTATGCCATTAATAAGTAAACGGAATGCAGATTTTCTTC
ATTTTGAGGTGCCAGGTATTACGCTACCGGAAGCCGTAAGGGAGAGGATGGATGGTCATGAAACGAAAGAGGCGGCAATT
GAGGAAGGAATTCGTATTTCACAAGAGTTGATTGATGAGACGATGAAATATTTTAACGGTATTTATCTTATTACGCCATT
TCTAAAATATGAAATTACAGAACATCTTGTTAAATATGTGAGAGAAAAGCAAGAAGTGAAAGAAGGTATTAATTGA

Upstream 100 bases:

>100_bases
CTGCAAAACGAATGTTTTGCATAATAAGAGGAGGAACAATTATGTTCCCCTCTTCAAAGTGAAGAGGGGGTTTTTATATT
GATAGAAATGAGGGAGATTT

Downstream 100 bases:

>100_bases
TGAAATGTATTGTATAGAAGAAAAATTACAAAATAACATCTTACTATTAGATGGTGCAATGGGAACGATGATCCAGCAAG
AGGACTTAACTGCGGAAGAT

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEAFKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLL
GLSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKR
MERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAI
EEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN

Sequences:

>Translated_611_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEAFKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLL
GLSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKR
MERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAI
EEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN
>Mature_611_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEAFKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLL
GLSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKR
MERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAI
EEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=324, Percent_Identity=27.4691358024691, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI13162290, Length=301, Percent_Identity=25.9136212624585, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157266337, Length=307, Percent_Identity=26.0586319218241, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1790450, Length=325, Percent_Identity=28.6153846153846, Blast_Score=112, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=28.2674772036474, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67399; Mature: 67399

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEECC
TYGANEAKLRMYGLENQVTEINRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMERE
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCEEECCHHHH
FMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKETNIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
DVNEILKQLLDYGANVVGLNCQLGPLHMTEAFKMISIPKNGYLSAYPNAGLPNYVEGRYV
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHEECCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVH
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
THEKRSKAHVTLAEKAKKQTTVVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSL
CHHHCCHHEEEHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
ASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSALGMEEVLALTGDPARV
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCC
GDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKR
CCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHH
MERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGI
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCC
TLPEAVRERMDGHETKEAAIEEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYV
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
REKQEVKEGIN
HHHHHHHHCCC
>Mature Secondary Structure
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEECC
TYGANEAKLRMYGLENQVTEINRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMERE
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHCCCEEECCHHHH
FMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKETNIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
DVNEILKQLLDYGANVVGLNCQLGPLHMTEAFKMISIPKNGYLSAYPNAGLPNYVEGRYV
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHEECCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVH
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
THEKRSKAHVTLAEKAKKQTTVVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSL
CHHHCCHHEEEHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
ASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSALGMEEVLALTGDPARV
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCC
GDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKR
CCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHH
MERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGI
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCC
TLPEAVRERMDGHETKEAAIEEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYV
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
REKQEVKEGIN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]