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The map label for this gene is citS [H]

Identifier: 227816570

GI number: 227816570

Start: 3671564

End: 3673168

Strand: Reverse

Name: citS [H]

Synonym: BAMEG_4027

Alternate gene names: 227816570

Gene position: 3673168-3671564 (Counterclockwise)

Preceding gene: 227816571

Following gene: 227816569

Centisome position: 70.23

GC content: 34.7

Gene sequence:

>1605_bases
TTGAAATTTAAAAAATTAAAACTACAACCGAGAATTACATTAACTATTAGTGCACTTATTTTAGTTGTACTTATGTTAAC
GAGTTATTTATTTTATTACATATTATCTGAAACAGTAGAAGAGCAAATTGGCAAAAGAGCTTTGCATGTTGCGAAAACAG
TTGCTGCTATACCAGAAATAAAGGAAGCTTTTCAAACAGAAAATCCAGCTTCTATCATTCAGCCAATTGCAGAAAGAATT
CGAATGGATACAGAGGCTGACTTCATTGTAGTTGGAAATAAAGAGGGGATTCGATATGCACATCCTGAGCGAGATAAAAT
AGGAGAAGCGATGATTGGTGGAGATAACAAAGGAGTTCTATTAGAAGGAAAATCATATGTTTCGAAAGCAACTGGATCAT
TAGGCCCTTCATTACGTGGAAAAGTTCCAATCCGTAATCAGGAAAATGAAATCATTGGTGTAGTGTCTGTTGGATTTTCA
ATGGATGATATTCATGGAGCGGTAGAAGTGTATGGAAAGCGTGTGTTTTGGATTACAATAATAGGTCTATTAATTGGAGT
AATAGGCTCTATATATTTAGCTGGCAGTATAAAAAGAATGATGTTTGGGATGGAGCCAGAAGAAATTTCATCTTTATATG
AAGAGCATAGTACAGTGATTCAATCTGTACGTGAAGGGATTATCGTCATTGATCAAAATGGCATGATTAGTTTAGTCAAT
CAAGCAGCCTATGATATTCTTGCGCTTGATGAACAACGAAATATTATTGGAGAATTTATTTTAGATATAATTCCTAATTC
AACGATATTAGATGTACTTCAAACTGGTGAAGAACAGTTTGATCGTCAATTAAATATAAAAGGGCAGGCTGTTATTGCGA
ACCGTCTACCCATTAAAGTGAAAAATAAAGTAACTGGCGTTGTATCGAGCTTGCGTTTGAAATCTGAAATGGATCAGTTA
ACAGCAGAGTTATCTCAGACGAAGCAATATACAGAGGCATTACGAGCGCAAACACATGAATATAACAATTTATTGTATAC
GCTTTCAGGTCTTATCCAGCTAGAATTATATGAAGATGCTTTGGAGCTTATTCATAAGGAAACAGCGGTATATCAAGATT
TTGTTCAATTTATTATGAAACGAATTCAAAATCCATGGTTAGGAGGGATTTTAATTGGATTCTATAATAGAGCAAGAGAG
TTGAAGATTGAGTTTATGCTAGATAGAGAGAGTAGCTTAGACAAATTAAGCCCGCCTATTGAGAGTAATTATGTCGTTTC
TATTTTAGGAAATTTAATTACGAATGCATTTGAAGCGATTGAAAGAAATGAAGAACATGATAAGAAAGTAAGAATGTTTG
TAACTGATATCGGAGAAGAAATAGTCATTGAAGTGGAAGACTCAGGACAAGGGATTCATGATGAGGTCATTACTAGCATA
TTCTATAAAGGCTTCTCGACGAAAGAAGGCGAGAAGCGAGGATATGGATTAGCAAAAGTGAAAGAGTTAGTAGAAGATTT
AAATGGAAGTATCGCAATCGAAAAAGGGGATTTAGGTGGTGCATTGTTTATTATTGCACTACCAAAAGAGAGGGGCGATT
TGTAA

Upstream 100 bases:

>100_bases
AAAAAGACAGAAATATAGAGTAACCGTGTTATGATTAAGTATAATTGAAGAGACTGTATATTCTTTAAATTCTATCTTTA
ATCATGTTAGGGGAATGGTT

Downstream 100 bases:

>100_bases
TGGGGGAGGAGATTGAAGTATTGATTGTAGAAGATGATATTCGAATTGCGGATATTCATCGTCGTTTTACTGAAAAGATT
GAGGGATTTAAAGTAATTGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKFKKLKLQPRITLTISALILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILALDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIEFMLDRESSLDKLSPPIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVITSI
FYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL

Sequences:

>Translated_534_residues
MKFKKLKLQPRITLTISALILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILALDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIEFMLDRESSLDKLSPPIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVITSI
FYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
>Mature_534_residues
MKFKKLKLQPRITLTISALILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILALDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIEFMLDRESSLDKLSPPIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVITSI
FYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=31.9626168224299, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1790567, Length=519, Percent_Identity=32.1772639691715, Blast_Score=279, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59713; Mature: 59713

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKKLKLQPRITLTISALILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQTENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILALDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIEFMLDRESSLDKLSPPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEEEEEEEECCCCCHHHCCCCC
ESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVITSI
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKFKKLKLQPRITLTISALILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQTENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILALDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIEFMLDRESSLDKLSPPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEEEEEEEECCCCCHHHCCCCC
ESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVITSI
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]