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The map label for this gene is mdaB [H]

Identifier: 227816291

GI number: 227816291

Start: 3392902

End: 3393450

Strand: Reverse

Name: mdaB [H]

Synonym: BAMEG_3719

Alternate gene names: 227816291

Gene position: 3393450-3392902 (Counterclockwise)

Preceding gene: 227816292

Following gene: 227816289

Centisome position: 64.88

GC content: 32.79

Gene sequence:

>549_bases
ATGAAAAATATATTTATTATAAATGGACATGAAAAATACGGTACAAAGGAAGGACGATTAAATAAAACGTTAGTAGATCA
TATGGTGACGGTATTAAGCGAGAACCATCAAGTGAAAACAACAACGATTCAAGATGGATACAATATAAAAGAAGAACAAG
ATAAATTTTTATGGGCTGATGTTGTGATTTATCAAACACCTATTTATTGGTTTAGTGTTCCAGGATTATTTAAAACGTAT
ATGGATGAAGTGTATGAATACGGTTTGTTCTTTAAAGGTGCAGATGAATATGGAACAGGTGGTTTATTAACAGAGAAAGA
GTATATGTTCTCTACAACTTGGAATGCACCTGAAAATTCATTTGGAGATAAGACGAAGTTCTTTGAAGGGGAAAGTCTAG
AATCAACGCTTAGTCATTTACACCGCGTGCAGAAGTTTCTTGGGATGAGTCCGTTAAAGAGTTTTGCTTGTTATGATGTG
GTGAAGAATCCGGATATTGAGCAATATTTATTGAACTTGAAAGATCACTTAGATGAAGTAATTAAATAA

Upstream 100 bases:

>100_bases
GACGGAAAGATTACGGAAGACTGGCATTTAGAAGATAATCTTACGTTGAAACAGCAACTTGGGTTAATAAACGAGGAATA
GAGTAGGGGAGAGAAAAACG

Downstream 100 bases:

>100_bases
TAGTTTATATTTGTATGTGAAGAAGGTACTACTAAGGATGTAGTACCTTCTCCTTTTATAATGAATCGCATTTTAAGACA
TGGATGGCTGTATCCATAAC

Product: NAD(P)H dehydrogenase, quinone family

Products: NADP; a reduced quinone; reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKNIFIINGHEKYGTKEGRLNKTLVDHMVTVLSENHQVKTTTIQDGYNIKEEQDKFLWADVVIYQTPIYWFSVPGLFKTY
MDEVYEYGLFFKGADEYGTGGLLTEKEYMFSTTWNAPENSFGDKTKFFEGESLESTLSHLHRVQKFLGMSPLKSFACYDV
VKNPDIEQYLLNLKDHLDEVIK

Sequences:

>Translated_182_residues
MKNIFIINGHEKYGTKEGRLNKTLVDHMVTVLSENHQVKTTTIQDGYNIKEEQDKFLWADVVIYQTPIYWFSVPGLFKTY
MDEVYEYGLFFKGADEYGTGGLLTEKEYMFSTTWNAPENSFGDKTKFFEGESLESTLSHLHRVQKFLGMSPLKSFACYDV
VKNPDIEQYLLNLKDHLDEVIK
>Mature_182_residues
MKNIFIINGHEKYGTKEGRLNKTLVDHMVTVLSENHQVKTTTIQDGYNIKEEQDKFLWADVVIYQTPIYWFSVPGLFKTY
MDEVYEYGLFFKGADEYGTGGLLTEKEYMFSTTWNAPENSFGDKTKFFEGESLESTLSHLHRVQKFLGMSPLKSFACYDV
VKNPDIEQYLLNLKDHLDEVIK

Specific function: Unknown

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli madB and S.pombe SPAC5H10.05c [H]

Homologues:

Organism=Escherichia coli, GI1789406, Length=191, Percent_Identity=37.696335078534, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: NA

Molecular weight: Translated: 21181; Mature: 21181

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIFIINGHEKYGTKEGRLNKTLVDHMVTVLSENHQVKTTTIQDGYNIKEEQDKFLWAD
CCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
VVIYQTPIYWFSVPGLFKTYMDEVYEYGLFFKGADEYGTGGLLTEKEYMFSTTWNAPENS
HHEEECCHHEEECHHHHHHHHHHHHHHCCEEECCCCCCCCCCEECCHHEEEECCCCCCCC
FGDKTKFFEGESLESTLSHLHRVQKFLGMSPLKSFACYDVVKNPDIEQYLLNLKDHLDEV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IK
CC
>Mature Secondary Structure
MKNIFIINGHEKYGTKEGRLNKTLVDHMVTVLSENHQVKTTTIQDGYNIKEEQDKFLWAD
CCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
VVIYQTPIYWFSVPGLFKTYMDEVYEYGLFFKGADEYGTGGLLTEKEYMFSTTWNAPENS
HHEEECCHHEEECHHHHHHHHHHHHHHCCEEECCCCCCCCCCEECCHHEEEECCCCCCCC
FGDKTKFFEGESLESTLSHLHRVQKFLGMSPLKSFACYDVVKNPDIEQYLLNLKDHLDEV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; a quinone; Acceptor; NADPH [C]

Specific reaction: NADPH + a quinone = NADP + a reduced quinone Acceptor + NADPH = reduced acceptor + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]